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. 1980 Nov;36(2):586–590. doi: 10.1128/jvi.36.2.586-590.1980

Incorporation of influenza virus M-protein into liposomes.

D J Bucher, I G Kharitonenkov, J A Zakomirdin, V B Grigoriev, S M Klimenko, J F Davis
PMCID: PMC353677  PMID: 7431489

Abstract

M-protein from influenza virus vaccine was purified by sodium dodecyl sulfate-gel chromatography and incorporated into liposomes by solubilization with octylglucoside and subsequent dialysis. Liposomes containing M-protein formed a distinct population with a density of 1.22 g/ml on sucrose-gradient centrifugation, regardless of the net charge on the liposomes. Treatment of the liposomes by freeze-fracture followed by electron microscopic examination showed multilamellar structures in those liposomes without M-protein; liposomes containing M-protein were mulberry-like structures which appeared unilamellar. These studies show incorporation of M-protein into the lipid bilayer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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