Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Dec;36(3):837–846. doi: 10.1128/jvi.36.3.837-846.1980

Nucleic acids of respiratory syncytial virus.

D M Lambert, M W Pons, G N Mbuy, K Dorsch-Hasler
PMCID: PMC353711  PMID: 7463558

Abstract

Analysis of purified respiratory syncytial virus revealed that the virion RNA was composed of 50S, 28S, 18S, and 4S species. The 18S and 28S species were presumed to represent host rRNA since virus grown in actinomycin D-treated cells contained only 50S and 4S RNAs. Actinomycin D treatment stimulated production of infectious respiratory syncytial virus 5- to 10-fold. The 50S virion RNA was shown to hybridize with polyadenylated mRNA's isolated from infected cells, indicating that respiratory syncytial virus RNA is of negative-strand sense. Six mRNA's were identified by polyacrylamide gel electrophoresis.

Full text

PDF
837

Images in this article

840Image
on p.840
845Image
on p.845

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Astell C., Smith M. Thermal elution of complementary sequences of nucleic acids from cellulose columns with covalently attached oligonucleotides of known length and sequence. J Biol Chem. 1971 Mar 25;246(6):1944–1946. [PubMed] [Google Scholar]
  2. BLOTH B., ESPMARK A., NORRBY E., GARD S. THE ULTRASTRUCTURE OF RESPIRATORY SYNCYTIAL (RS) VIRUS. (BRIEF REPORT). Arch Gesamte Virusforsch. 1963 Aug 26;13:582–586. doi: 10.1007/BF01267800. [DOI] [PubMed] [Google Scholar]
  3. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  4. CHANOCK R., ROIZMAN B., MYERS R. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am J Hyg. 1957 Nov;66(3):281–290. doi: 10.1093/oxfordjournals.aje.a119901. [DOI] [PubMed] [Google Scholar]
  5. Cash P., Pringle C. R., Preston C. M. The polypeptides of human respiratory syncytial virus: products of cell-free protein synthesis and post-translational modifications. Virology. 1979 Jan 30;92(2):375–384. doi: 10.1016/0042-6822(79)90142-9. [DOI] [PubMed] [Google Scholar]
  6. Chanock R. M. Control of acute mycoplasmal and viral respiratory tract disease. Science. 1970 Jul 17;169(3942):248–256. doi: 10.1126/science.169.3942.248. [DOI] [PubMed] [Google Scholar]
  7. Desselberger U., Palese P. Molecular weights of RNA segments of influenza A and B viruses. Virology. 1978 Jul 15;88(2):394–399. doi: 10.1016/0042-6822(78)90297-0. [DOI] [PubMed] [Google Scholar]
  8. Eckert E. A., Maassab H. F., Cavallaro J. J. Inner component of respiratory syncytial virus. J Bacteriol. 1965 Dec;90(6):1784–1785. doi: 10.1128/jb.90.6.1784-1785.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Etkind P. R., Buchhagen D. L., Herz C., Broni B. B., Krug R. M. The segments of influenza viral mRNA. J Virol. 1977 May;22(2):346–352. doi: 10.1128/jvi.22.2.346-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Etkind P. R., Krug R. M. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts. J Virol. 1975 Dec;16(6):1464–1475. doi: 10.1128/jvi.16.6.1464-1475.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gimenez H. B., Pringle C. R. Seven complementation groups of respiratory syncytial virus temperature-sensitive mutants. J Virol. 1978 Sep;27(3):459–464. doi: 10.1128/jvi.27.3.459-464.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hodes D. S., Schauf V., Chanock R. M. Isolation of RNA species from HeLa cells infected with respiratory syncytial virus. Proc Soc Exp Biol Med. 1974 May;146(1):287–290. doi: 10.3181/00379727-146-38089. [DOI] [PubMed] [Google Scholar]
  13. Joncas J., Berthiaume L., Pavilanis V. The structure of the respiratory syncytial virus. Virology. 1969 Jul;38(3):493–496. doi: 10.1016/0042-6822(69)90166-4. [DOI] [PubMed] [Google Scholar]
  14. Kolakofsky D., Bruschi A. Antigenomes in Sendai virions and Sendai virus-infected cells. Virology. 1975 Jul;66(1):185–191. doi: 10.1016/0042-6822(75)90189-0. [DOI] [PubMed] [Google Scholar]
  15. Levine S. Polypeptides of respiratory syncytial virus. J Virol. 1977 Jan;21(1):427–431. doi: 10.1128/jvi.21.1.427-431.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Melnick J. L. Classification and nomenclature of animal viruses, 1971. Prog Med Virol. 1971;13:462–484. [PubMed] [Google Scholar]
  17. Perry R. P., Kelley D. E. Inhibition of RNA synthesis by actinomycin D: characteristic dose-response of different RNA species. J Cell Physiol. 1970 Oct;76(2):127–139. doi: 10.1002/jcp.1040760202. [DOI] [PubMed] [Google Scholar]
  18. Pons M. W. A reexamination of influenza single-and double-stranded RNAs by gel electrophoresis. Virology. 1976 Feb;69(2):789–792. doi: 10.1016/0042-6822(76)90508-0. [DOI] [PubMed] [Google Scholar]
  19. Richman A. V., Pedreira F. A., Tauraso N. M. Attempts to demonstrate hemagglutination and hemadsorption by respiratory syncytial virus. Appl Microbiol. 1971 Jun;21(6):1099–1100. doi: 10.1128/am.21.6.1099-1100.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Simpson R. W., Iinuma M. Recovery of infectious proviral DNA from mammalian cells infected with respiratory syncytial virus. Proc Natl Acad Sci U S A. 1975 Aug;72(8):3230–3234. doi: 10.1073/pnas.72.8.3230. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wunner W. H., Pringle C. R. Respiratory syncytial virus proteins. Virology. 1976 Aug;73(1):228–243. doi: 10.1016/0042-6822(76)90077-5. [DOI] [PubMed] [Google Scholar]
  22. Zakstelskaya L. Y., Almeida J. D., Bradstreet C. M. The morphological characterisation of respiratory syncytial virus by a simple electron microscope technique. Acta Virol. 1967 Sep;11(5):420–423. [PubMed] [Google Scholar]
  23. Zhdanov V. M., Dreizin R. S., Yankevici O. D., Astakhova A. K. Biophysical characteristics of respiratory syncytial virus. Rev Roum Virol. 1974;25(3):277–280. [PubMed] [Google Scholar]
  24. de Jong J. C., Harmsen M. Stimulated growth of respiratory syncytial virus in the presence of actinomycin D. Antonie Van Leeuwenhoek. 1973;39(3):409–413. doi: 10.1007/BF02578883. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES