Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Jan;21(1):41–53. doi: 10.1128/jvi.21.1.41-53.1977

Rifampin-susceptible mutant of vesicular stomatitis virus: protein and RNA synthesis.

M C Moreau, B Sanzey
PMCID: PMC353789  PMID: 189073

Abstract

A rifampin-susceptible strain (VSV Rif+) was selected from the wild vesicular stomatitis virus (VSV) population unsusceptible to rifampin. The VSV Rif+ was blocked in its intracellular replication in the presence of rifampin. In cells, rifampin affected primarily VSV Rif+ transcription, but to a different extent than in a cell-free system. In addition, a decrease in the amount of VSV Rif+ protein M was detected, linked to a stimulation of protein NS. In the absence of rifampin, protein M, although synthesized, was not immediately incorporated into the cell membrane. An interpretation of these observations is proposed.

Full text

PDF
41

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Both G. W., Moyer S. A., Banerjee A. K. Translation and identification of the viral mRNA species isolated from subcellular fractions of vesicular stomatitis virus-infected cells. J Virol. 1975 Apr;15(4):1012–1019. doi: 10.1128/jvi.15.4.1012-1019.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. David A. E. Assembly of the vesicular stomatitis virus envelope: incorporation of viral polypeptides into the host plasma membrane. J Mol Biol. 1973 May 5;76(1):135–148. doi: 10.1016/0022-2836(73)90085-5. [DOI] [PubMed] [Google Scholar]
  3. Ehrenfeld E., Summers D. F. Adenylate-rich sequences in vesicular stomatitis virus messenger ribonucleic acid. J Virol. 1972 Oct;10(4):683–688. doi: 10.1128/jvi.10.4.683-688.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Emerson S. U., Yu Y. Both NS and L proteins are required for in vitro RNA synthesis by vesicular stomatitis virus. J Virol. 1975 Jun;15(6):1348–1356. doi: 10.1128/jvi.15.6.1348-1356.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hartmann G., Honikel K. O., Knüsel F., Nüesch J. The specific inhibition of the DNA-directed RNA synthesis by rifamycin. Biochim Biophys Acta. 1967;145(3):843–844. doi: 10.1016/0005-2787(67)90147-5. [DOI] [PubMed] [Google Scholar]
  6. Heller E., Argaman M., Levy H., Goldblum N. Selective inhibition of vaccinia virus by the antibiotic rifampicin. Nature. 1969 Apr 19;222(5190):273–274. doi: 10.1038/222273a0. [DOI] [PubMed] [Google Scholar]
  7. Huang A. S., Manders E. K. Ribonucleic acid synthesis of vesicular stomatitis virus. IV. Transcription by standard virus in the presence of defective interfering particles. J Virol. 1972 Jun;9(6):909–916. doi: 10.1128/jvi.9.6.909-916.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Huppert J., Gresland L., Rosenbergova M. Newcastle disease virus: virus particle and RNA synthesis in different host cells and at different temperatures. J Gen Virol. 1974 Jan;22(1):129–142. doi: 10.1099/0022-1317-22-1-129. [DOI] [PubMed] [Google Scholar]
  9. Kang C. Y., Prevec L. Proteins of vesicular stomatitis virus. 3. Intracellular synthesis and extracellular appearance of virus-specific proteins. Virology. 1971 Dec;46(3):678–690. doi: 10.1016/0042-6822(71)90070-5. [DOI] [PubMed] [Google Scholar]
  10. Kiley M. P., Wagner R. R. Ribonucleic acid species of intracellular nucleocapsids and released virions of vesicular stomatitis virus. J Virol. 1972 Aug;10(2):244–255. doi: 10.1128/jvi.10.2.244-255.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lindberg U., Persson T. Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose. Eur J Biochem. 1972 Dec 4;31(2):246–254. doi: 10.1111/j.1432-1033.1972.tb02527.x. [DOI] [PubMed] [Google Scholar]
  12. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Marcus P. I., Engelhardt D. L., Hunt J. M., Sekellick M. J. Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science. 1971 Nov 5;174(4009):593–598. doi: 10.1126/science.174.4009.593. [DOI] [PubMed] [Google Scholar]
  14. McSharry J. J., Wagner R. R. Lipid composition of purified vesicular stomatitis viruses. J Virol. 1971 Jan;7(1):59–70. doi: 10.1128/jvi.7.1.59-70.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Moreau M. C. Action de la rifampicine sur le virus de la stomatite vésiculaire. C R Acad Sci Hebd Seances Acad Sci D. 1972 Jan 24;274(4):611–613. [PubMed] [Google Scholar]
  16. Moreau M. C. Inhibition of a vesicular stomatitis virus mutant by rifampin. J Virol. 1974 Sep;14(3):517–521. doi: 10.1128/jvi.14.3.517-521.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Printz P., Wagner R. R. Temperature-sensitive mutants of vesicular stomatitis virus: synthesis of virus-specific proteins. J Virol. 1971 May;7(5):651–662. doi: 10.1128/jvi.7.5.651-662.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. II. Separation and characterization of virus-specific RNA species. Virology. 1972 Sep;49(3):766–783. doi: 10.1016/0042-6822(72)90533-8. [DOI] [PubMed] [Google Scholar]
  19. Soria M., Huang A. S. Association of polyadenylic acid with messenger RNA of vesicular stomatitis virus. J Mol Biol. 1973 Jul 5;77(3):449–455. doi: 10.1016/0022-2836(73)90450-6. [DOI] [PubMed] [Google Scholar]
  20. Stender W., Stütz A. A., Scheit K. H. The modification of DNA-dependent RNA polymerase from Escherichia coli by an alkylating derivative of rifamycin SV. Eur J Biochem. 1975 Aug 1;56(1):129–136. doi: 10.1111/j.1432-1033.1975.tb02215.x. [DOI] [PubMed] [Google Scholar]
  21. Subak-Sharpe J. H., Timbury M. C., Williams J. F. Rifampicin inhibits the growth of some mammalian viruses. Nature. 1969 Apr 26;222(5191):341–345. doi: 10.1038/222341a0. [DOI] [PubMed] [Google Scholar]
  22. Szilágyi J. F., Pringle C. R. Effect of temperature-sensitive mutations on the virion-associated RNA transcriptase of vesicular stomatitis virus. J Mol Biol. 1972 Nov 14;71(2):281–291. doi: 10.1016/0022-2836(72)90351-8. [DOI] [PubMed] [Google Scholar]
  23. Unger J. T., Reichmann M. E. RNA synthesis in temperature-sensitive mutants of vesicular stomatitis virus. J Virol. 1973 Sep;12(3):570–578. doi: 10.1128/jvi.12.3.570-578.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wagner R. R., Kiley M. P., Snyder R. M., Schnaitman C. A. Cytoplasmic compartmentalization of the protein and ribonucleic acid species of vesicular stomatitis virus. J Virol. 1972 Apr;9(4):672–683. doi: 10.1128/jvi.9.4.672-683.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wagner R. R., Schnaitman T. C., Snyder R. M., Schnaitman C. A. Protein composition of the structural components of vesicular stomatitis virus. J Virol. 1969 Jun;3(6):611–618. doi: 10.1128/jvi.3.6.611-618.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wagner R. R., Snyder R. M., Yamazaki S. Proteins of vesicular stomatitis virus: kinetics and cellular sites of synthesis. J Virol. 1970 May;5(5):548–558. doi: 10.1128/jvi.5.5.548-558.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES