Abstract
A soluble polymerase-template complex prepared from poliovirus-infected cells was found to incorporate radioactive UTP into trichloroacetic acid-insoluble RNA linearly for 8 h in the presence of ATP and Mg2+. Radioactive CTP or GTP was not incorporated under identical conditions. Nearest-neighbor analysis of the in vitro product demonstrated that ATP was added to the viral RNA in the form of polyadenylic acid; UTP was added internally to the 3'-OH group of all four nucelotides. The data can best be explained by the addition of the UTP to the 3'-OH groups of single-stranded breaks in the double-stranded viral RNA and ligation to the adjacent 5'-phosphate groups. The enzymatic activity was also found in encephalomyocarditis virus- and rhinovirus type 1A-infected cells but not in uninfected cells.
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- Bedows E., Wachsman J. T., Gumport R. I. Absence of detectable RNA ligase activity in eukaryotic cells. Biochem Biophys Res Commun. 1975 Dec 1;67(3):1100–1107. doi: 10.1016/0006-291x(75)90787-1. [DOI] [PubMed] [Google Scholar]
- Dorsch-Häsler K., Yogo Y., Wimmer E. Replication of picornaviruses. I. Evidence from in vitro RNA synthesis that poly(A) of the poliovirus genome is genetically coded. J Virol. 1975 Dec;16(6):1512–1517. doi: 10.1128/jvi.16.6.1512-1517.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ehrenfeld E., Maizel J. V., Summers D. F. Soluble RNA polymerase complex from poliovirus-infected HeLa cells. Virology. 1970 Apr;40(4):840–846. doi: 10.1016/0042-6822(70)90129-7. [DOI] [PubMed] [Google Scholar]
- Goulian M. Some recent developments in DNA enzymology. Prog Nucleic Acid Res Mol Biol. 1972;12:29–48. doi: 10.1016/s0079-6603(08)60658-3. [DOI] [PubMed] [Google Scholar]
- Hall L., Rueckert R. R. Infection of mouse fibroblasts by cardioviruses: premature uncoating and its prevention by elevated pH and magnesium chloride. Virology. 1971 Jan;43(1):152–165. doi: 10.1016/0042-6822(71)90233-9. [DOI] [PubMed] [Google Scholar]
- Linné T., Oberg B., Philipson L. RNA ligase activity in phage-infected bacteria and animal cells. Eur J Biochem. 1974 Feb 15;42(1):157–165. doi: 10.1111/j.1432-1033.1974.tb03325.x. [DOI] [PubMed] [Google Scholar]
- Lundquist R. E., Ehrenfeld E., Maizel J. V., Jr Isolation of a viral polypeptide associated with poliovirus RNA polymerase. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773–4777. doi: 10.1073/pnas.71.12.4773. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Spector D. H., Baltimore D. Polyadenylic acid on poliovirus RNA. III. In vitro addition of polyadenylic acid to poliovirus RNAs. J Virol. 1975 Jun;15(6):1432–1439. doi: 10.1128/jvi.15.6.1432-1439.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yin F. H., Knight E., Jr In vivo and in vitro synthesis of human rhinovirus type 2 ribonucleic acid. J Virol. 1972 Jul;10(1):93–98. doi: 10.1128/jvi.10.1.93-98.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yogo Y., Wimmer E. Sequence studies of poliovirus RNA. III. Polyuridylic acid and polyadenylic acid as components of the purified poliovirus replicative intermediate. J Mol Biol. 1975 Mar 5;92(3):467–477. doi: 10.1016/0022-2836(75)90292-2. [DOI] [PubMed] [Google Scholar]