Abstract
The nature and theory of flow field-flow fractionation is described, and its potential applicability to virus-like particles is discussed. Different virus types are shown to be retained at different levels. Retention can be controlled by variation of the experimental parameters, in good agreement with theory. However, a mild adsorption effect is indicated and requires the development of alternate strategies for measuring diffusion coefficients. For Qbeta, our value agrees well within 10% of literature values; the values obtained for other viruses, using Abeta as an internal standard, are untested. Finally, it is demonstrated that flow field-flow fractionation can cleanly fractionate two viruses from one another and from an albumin impurity, that samples as large as several milligrams in size can be analyzed, and that the method has potential utility in the quantitative and qualitative analysis of virus systems.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caldwell K. D., Kesner L. F., Myers M. N., Giddings J. C. Electrical field-flow fractionation of proteins. Science. 1972 Apr 21;176(4032):296–298. doi: 10.1126/science.176.4032.296. [DOI] [PubMed] [Google Scholar]
- Camerini-Otero R. D., Pusey P. N., Koppel D. E., Schaefer D. W., Franklin R. M. Intensity fluctuation spectroscopy of laser light scattered by solutions of spherical viruses: R17, Q beta, BSV, PM2, and T7. II. Diffusion coefficients, molecular weights, solvation, and particle dimensions. Biochemistry. 1974 Feb 26;13(5):960–970. doi: 10.1021/bi00702a021. [DOI] [PubMed] [Google Scholar]
- MOELLER W. J. DETERMINATION OF DIFFUSION COEFFICIENTS AND MOLECULAR WEIGHTS OF RIBONUCLEIC ACIDS AND VIRUSES. Proc Natl Acad Sci U S A. 1964 Mar;51:501–509. doi: 10.1073/pnas.51.3.501. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Overby L. R., Barlow G. H., Doi R. H., Jacob M., Spiegelman S. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles. J Bacteriol. 1966 Jan;91(1):442–448. doi: 10.1128/jb.91.1.442-448.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- STRAUSS J. H., Jr, SINSHEIMER R. L. Purification and properties of bacteriophage MS2 and of its ribonucleic acid. J Mol Biol. 1963 Jul;7:43–54. doi: 10.1016/s0022-2836(63)80017-0. [DOI] [PubMed] [Google Scholar]