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. 1977 Jan;21(1):139–146. doi: 10.1128/jvi.21.1.139-146.1977

Steroid induction of mouse mammary tumor virus: effect upon synthesis and degradation of viral RNA.

H A Young, T Y Shih, E M Scolnick, W P Parks
PMCID: PMC353799  PMID: 189061

Abstract

Steroid hormones have been demonstrated to induce in tissue culture the production of mouse mammary tumor viral (MMTV) RNA, proteins, and particles 10-fold compared with constitutive levels. However, previous data of increased viral RNA levels did not distinguish between an increased rate of viral-specific RNA synthesis and a slower rate of viral RNA degradation. According to the recently developed assay of Coffin et al. (1974) for measuring rates of viral RNA synthesis, short-term labeling experiments of a mouse mammary tumor cell line indicate that the glucocorticoid hormone dexamethasone stimulates a 3-fold increase in the synthesis of MMTV-specific RNA within 10 min after the addition of hormone and that stimulation of RNA synthesis reaches 5- to 10-fold within 30 to 60 min, while the synthesis of Moloney leukemia virus-specific RNA in the same cell is unaffected by steroids. The decay rates of pulse-labeled and accumulated MMTV RNA in the presence or absence of dexamethasone show this RNA to have a half-life of greater than 8 h. In addition, hormone-stimulated MMTV RNA appears to have an increased rate of decay compared to basal MMTV RNA, thus ruling out an increased stability of MMTV RNA in the presence of steroid hormones as the basis for increased RNA levels. Thus, the magnitude, rapidity, and specificity of hormone action on MMTV RNA synthesis indicate a primary effect upon transcription.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benveniste R. E., Scolnick E. M. RNA in mammalian sarcoma virus transformed nonproducer cells homologous to murine leukemia virus RNA. Virology. 1973 Feb;51(2):370–382. doi: 10.1016/0042-6822(73)90436-4. [DOI] [PubMed] [Google Scholar]
  2. Birnstiel M. L., Sells B. H., Purdom I. F. Kinetic complexity of RNA molecules. J Mol Biol. 1972 Jan 14;63(1):21–39. doi: 10.1016/0022-2836(72)90519-0. [DOI] [PubMed] [Google Scholar]
  3. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  4. Coffin J. M., Parsons J. T., Rymo L., Haroz R. K., Weissmann C. A new approach to the isolation of RNA-DNA hybrids and its application to the quantitative determination of labeled tumor virus RNA. J Mol Biol. 1974 Jun 25;86(2):373–396. doi: 10.1016/0022-2836(74)90026-6. [DOI] [PubMed] [Google Scholar]
  5. Dickson C., Haslam S., Nandi S. Conditions for optimal MTV synthesis in vitro and the effect of steroid hormones on virus production. Virology. 1974 Nov;62(1):242–252. doi: 10.1016/0042-6822(74)90319-5. [DOI] [PubMed] [Google Scholar]
  6. Fine D. L., Plowman J. K., Kelley S. P., Arthur L. O., Hillman E. A. Enhanced production of mouse mammary tumor virus in dexamethasone-treated, 5-iododeoxyuridine-stimulated mammary tumor cell cultures. J Natl Cancer Inst. 1974 Jun;52(6):1881–1886. doi: 10.1093/jnci/52.6.1881. [DOI] [PubMed] [Google Scholar]
  7. Goldsby R. A., Zipser E. The isolation and replica plating of mammalian cell clones. Exp Cell Res. 1969 Feb;54(2):271–275. doi: 10.1016/0014-4827(69)90250-x. [DOI] [PubMed] [Google Scholar]
  8. Leong J. A., Garapin A. C., Jackson N., Fanshier L., Levinson W., Bishop J. M. Virus-specific ribonucleic acid in cells producing rous sarcoma virus: detection and characterization. J Virol. 1972 Jun;9(6):891–902. doi: 10.1128/jvi.9.6.891-902.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Owens R. B., Hackett A. J. Tissue culture studies of mouse mammary tumor cells and associated viruses. J Natl Cancer Inst. 1972 Nov;49(5):1321–1332. [PubMed] [Google Scholar]
  10. Parks W. P., Hubbell E. S., Goldberg R. J., O'Neill F. J., Scolnick E. M. High frequency variation in mammary tumor virus expression in cell culture. Cell. 1976 May;8(1):87–93. doi: 10.1016/0092-8674(76)90189-6. [DOI] [PubMed] [Google Scholar]
  11. Parks W. P., Ransom J. C., Young H. A., Scolnick E. M. Mammary tumor virus induction by glucocorticoids. Characterization of specific transcriptional regulation. J Biol Chem. 1975 May 10;250(9):3330–3336. [PubMed] [Google Scholar]
  12. Parks W. P., Scolnick E. M., Kozikowski E. H. Dexamethasone stimulation of murine mammary tumor virus expression: a tissue culture source of virus. Science. 1974 Apr 12;184(4133):158–160. doi: 10.1126/science.184.4133.158. [DOI] [PubMed] [Google Scholar]
  13. Ringold G. M., Yamamoto K. R., Tomkins G. M., Bishop M., Varmus H. E. Dexamethasone-mediated induction of mouse mammary tumor virus RNA: a system for studying glucocorticoid action. Cell. 1975 Nov;6(3):299–305. doi: 10.1016/0092-8674(75)90181-6. [DOI] [PubMed] [Google Scholar]
  14. Ringold G., Lasfargues E. Y., Bishop J. M., Varmus H. E. Production of mouse mammary tumor virus by cultured cells in the absence and presence of hormones: assay by molecular hybridization. Virology. 1975 May;65(1):135–147. doi: 10.1016/0042-6822(75)90014-8. [DOI] [PubMed] [Google Scholar]
  15. Rymo L., Parsons J. T., Coffin J. M., Weissmann C. In vitro synthesis of Rous sarcoma virus-specific RNA is catalyzed by a DNA-dependent RNA polymerase. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2782–2786. doi: 10.1073/pnas.71.7.2782. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Scolnick E. M., Young H. A., Parks W. P. Biochemical and physiological mechanisms in glucocorticoid hormone induction of mouse mammary tumor virus. Virology. 1976 Jan;69(1):148–156. doi: 10.1016/0042-6822(76)90202-6. [DOI] [PubMed] [Google Scholar]
  17. Shyamala G. Glucocorticoid receptors in mouse mammary tumors. Specific binding of glucocorticoids in the cytoplasm. J Biol Chem. 1974 Apr 10;249(7):2160–2163. [PubMed] [Google Scholar]
  18. Sykes J. A., Whitescarver J., Briggs L. Observations on a cell line producing mammary tumor virus. J Natl Cancer Inst. 1968 Dec;41(6):1315–1327. [PubMed] [Google Scholar]
  19. Young H. A., Scolnick E. M., Parks W. P. Glucocorticoid-receptor interaction and induction of murine mammary tumor virus. J Biol Chem. 1975 May 10;250(9):3337–3343. [PubMed] [Google Scholar]

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