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. 1977 Jan;21(1):179–186. doi: 10.1128/jvi.21.1.179-186.1977

Common structural antigen of papovaviruses of the simian virus 40-polyoma subgroup.

K V Shah, H L Ozer, H N Ghazey, T J Kelly Jr
PMCID: PMC353804  PMID: 64625

Abstract

An antigenic determinant common to the major capsid polypeptide (VP1) of simian virus 40 (SV40) and polyoma virus is described. Antisera prepared against intact viral particles reacted only with cells infected with the homologous virus by immunofluorescence tests (IF). However, antisera prepared against disrupted SV40 particles reacted in IF with both polyoma- and SV40-infected permissive cells. The cross-reaction with polyoma was localized to VP1 by the following evidence. (i) The IF cross-reaction was inhibited by preincubation of the antiserum with purified SV40 VP1; (ii) purified radiolabeled polyoma VP1 was precipitated by the cross-reactive serum, and this reaction was inhibited by unlabeled SV40 VP1; (iii) other antisera prepared against purified SV40 VP1 or polyoma VP1 reacted in IF with both SV40- and polyma-infected permissive cells. These cross-reacting antisera also reacted in IF with permissive cells infected with BK virus, rabbit kidney vacuolating virus, and the stumptailed macaque virus, suggesting that all members of the polyoma-SV40 subgroup share a common antigenic determinant located in their major capsid polypeptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crawford L. V. Proteins of polyoma virus and SV40. Br Med Bull. 1973 Sep;29(3):253–258. doi: 10.1093/oxfordjournals.bmb.a071016. [DOI] [PubMed] [Google Scholar]
  2. Ferguson J., Davis R. W. An electron microscopic method for studying and mapping the region of weak sequence homology between simian virus 40 and polyoma DNAs. J Mol Biol. 1975 May 15;94(2):135–149. doi: 10.1016/0022-2836(75)90073-x. [DOI] [PubMed] [Google Scholar]
  3. Fried M. Isolation and partial characterization of different defective DNA molecules derived from polyoma virus. J Virol. 1974 May;13(5):939–946. doi: 10.1128/jvi.13.5.939-946.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  5. HARTLEY J. W., ROWE W. P. NEW PAPOVAVIRUS CONTAMINATING SHOPE PAPILLOMATA. Science. 1964 Jan 17;143(3603):258–260. doi: 10.1126/science.143.3603.258. [DOI] [PubMed] [Google Scholar]
  6. Johnson F. B., Blacklow N. R., Hoggan M. D. Immunological reactivity of antisera prepared against the sodium dodecyl sulfate-treated structural polypeptides of adenovirus-associated virus. J Virol. 1972 Jun;9(6):1017–1026. doi: 10.1128/jvi.9.6.1017-1026.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Khoury G., Howley P. M., Garon C., Mullarkey M. F., Takemoto K. K., Martin M. A. Homology and relationship between the genomes of papovaviruses, BK virus and simian virus 40. Proc Natl Acad Sci U S A. 1975 Jul;72(7):2563–2567. doi: 10.1073/pnas.72.7.2563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lodish H. F., Weinberg R., Ozer H. L. Translation of mRNA from simian virus 40-infected cells into simian virus 40 capsid protein by cell-free extracts. J Virol. 1974 Mar;13(3):590–595. doi: 10.1128/jvi.13.3.590-595.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MELNICK J. L. Papova virus group. Science. 1962 Mar 30;135(3509):1128–1130. doi: 10.1126/science.135.3509.1128. [DOI] [PubMed] [Google Scholar]
  10. May E., Kopecka H., May P. Mapping the transcription site of the SV40-specific late 16 S mRNA. Nucleic Acids Res. 1975 Oct;2(10):1995–2005. doi: 10.1093/nar/2.10.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Ozer H. L. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Ozer H. L., Tegtmeyer P. Synthesis and assembly of simian virus 40. II. Synthesis of the major capsid protein and its incorporation into viral particles. J Virol. 1972 Jan;9(1):52–60. doi: 10.1128/jvi.9.1.52-60.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  14. Penney J. B., Jr, Narayan O. Studies of the antigenic relationships of the new human papovaviruses by electron microscopy agglutination. Infect Immun. 1973 Aug;8(2):299–300. doi: 10.1128/iai.8.2.299-300.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROWE W. P., HARTLEY J. W., ESTES J. D., HUEBNER R. J. Studies of mouse polyoma virus infection. 1. Procedures for quantitation and detection of virus. J Exp Med. 1959 Apr 1;109(4):379–391. doi: 10.1084/jem.109.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rangan S. R., Lowrie R. C., Roberts J. A., Johnston P. B., Warrick R. P. Virus from stumptailed monkey (Macaca arctoides) kidney cultures. Lab Anim Sci. 1974 Feb;24(1):211–217. [PubMed] [Google Scholar]
  17. Reissig M., Kelly T. J., Jr, Daniel R. W., Rangan S. R., Shah K. V. Identification of the stumptailed macaque virus as a new papovavirus. Infect Immun. 1976 Jul;14(1):225–231. doi: 10.1128/iai.14.1.225-231.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shah K. V., Daniel R. W., Strandberg J. D. Sarcoma in a hamster inoculated with BK virus, a human papovavirus. J Natl Cancer Inst. 1975 Apr;54(4):945–950. [PubMed] [Google Scholar]
  19. Shah K. V., Willard S., Myers R. E., Hess D. M., DiGiacomo R. Experimental infection of rhesus with simian virus 40 (SV40). Proc Soc Exp Biol Med. 1969 Jan;130(1):196–203. doi: 10.3181/00379727-130-33520. [DOI] [PubMed] [Google Scholar]
  20. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  21. Takemoto K. K., Martin M. A. Transformation of hamster kidney cells by BK papovavirus DNA. J Virol. 1975 Jan;17(1):247–253. doi: 10.1128/jvi.17.1.247-253.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Tegtmeyer P., Robb J. A., Widmer C., Ozer H. L. Altered protein metabolism in infection by the late tsB11 mutant of simian virus 40. J Virol. 1974 Oct;14(4):997–1007. doi: 10.1128/jvi.14.4.997-1007.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Walker D. L., Padgett B. L., ZuRhein G. M., Albert A. E., Marsh R. F. Human papovavirus (JC): induction of brain tumors in hamsters. Science. 1973 Aug 17;181(4100):674–676. doi: 10.1126/science.181.4100.674. [DOI] [PubMed] [Google Scholar]
  24. Widmer C., Robb J. A. Simian virus 40-host cell interactions. II. Cytoplasmic and nucleolar accumulation of simian virus 40 virion protein. J Virol. 1974 Dec;14(6):1530–1546. doi: 10.1128/jvi.14.6.1530-1546.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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