Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1977 Jan;21(1):328–337. doi: 10.1128/jvi.21.1.328-337.1977

Viral protein synthesis in Friend erythroleukemia cell lines.

J Racevskis, G Koch
PMCID: PMC353819  PMID: 64627

Abstract

Viral protein synthesis was studied in two Friend virus-induced erythroleukemia cell lines (Ostertag cell lines FSD1-F4 and B8) by the technique of immuno-precipitation with monospecific antisera to the major envelope glycoprotein gp70 and major core protein p30. One of the cell lines (F4) releases active Friend virus complex to the growth medium, where release of virus from the other cell line (B8) is barely or nondetectable. It was found that in the nonproducer cell line B8, a large-molecular-weight protein of about 65,000 containing p30 antigenic determinants is synthesized, yet no p30 is produced upon prolonged incubation and chase, suggesting that this might be the actual lesion that prevents mature virus production by these cells. In both cell lines, the predominant protein species that is immunoprecipitated with monospecific anti-gp70 serum is a protein of 55,000 to 60,000 daltons that is labeled with glucosamine to a much lesser extent that gp70 and appears to become heterogeneous with time. Large amounts of gp70 can be detected in the cell-free medium, but none of the unstable species of 55,00 to 60,000 molecular weight.

Full text

PDF
328

Images in this article

331Image
on p.331
333Image
on p.333
334Image
on p.334

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolognesi D. P., Collins J. J., Leis J. P., Moennig V., Schäfer W., Atkinson P. H. Role of carbohydrate in determining the immunochemical properties of the major glycoprotein (gp71) of Friend murine leukemia virus. J Virol. 1975 Dec;16(6):1453–1463. doi: 10.1128/jvi.16.6.1453-1463.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner W. M., Laskey R. A. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974 Jul 1;46(1):83–88. doi: 10.1111/j.1432-1033.1974.tb03599.x. [DOI] [PubMed] [Google Scholar]
  3. Dube S. K., Pragnell I. B., Kluge N., Gaedicke G., Steinheider G., Ostertag W. Induction of endogenous and of spleen focus-forming viruses during dimethylsulfoxide-induced differentiation of mouse erythroleukemia cells transformed by spleen focus-forming virus. Proc Natl Acad Sci U S A. 1975 May;72(5):1863–1867. doi: 10.1073/pnas.72.5.1863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Eisenman R., Vogt V. M., Diggelmann H. Synthesis of avian RNA tumor virus structural proteins. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1067–1075. doi: 10.1101/sqb.1974.039.01.122. [DOI] [PubMed] [Google Scholar]
  5. Friend C., Scher W., Holland J. G., Sato T. Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1971 Feb;68(2):378–382. doi: 10.1073/pnas.68.2.378. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Halpern M. S., Bolognesi D. P., Lewandowski L. J. Isolation of the major viral glycoprotein and a putative precursor from cells transformed by avian sarcoma viruses. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2342–2346. doi: 10.1073/pnas.71.6.2342. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hayflick L. Tissue cultures and mycoplasmas. Tex Rep Biol Med. 1965 Jun;23(Suppl):285+–285+. [PubMed] [Google Scholar]
  8. Ikeda H., Hardy W., Jr, Tress E., Fleissner E. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. V. Identification of a new murine viral protein, p15(E). J Virol. 1975 Jul;16(1):53–61. doi: 10.1128/jvi.16.1.53-61.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Jamjoom G., Karshin W. L., Naso R. B., Arcement L. J., Arlinghaus R. B. Proteins of Rauscher murine leukemia virus: resolution of a 70,000-dalton, Nonglycosylated polypeptide containing p30 peptide sequences. Virology. 1975 Nov;68(1):135–145. doi: 10.1016/0042-6822(75)90155-5. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Moennig V., Frank H., Hunsmann G., Schneider I., Schafer W. Properties of mouse leukemia viruses. VII. The major viral glycoprotein of friend leukemia virus. Isolation and physicochemical properties. Virology. 1974 Sep;61(1):100–111. doi: 10.1016/0042-6822(74)90245-1. [DOI] [PubMed] [Google Scholar]
  12. Naso R. B., Arcement L. J., Karshin W. L., Jamjoom G. A., Arlinghaus R. B. A fucose-deficient glycoprotein precursor to Rauscher leukemia virus gp69/71. Proc Natl Acad Sci U S A. 1976 Jul;73(7):2326–2330. doi: 10.1073/pnas.73.7.2326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nuss D. L., Koch G. Differential inhibition of vesicular stomatitis virus polypeptide synthesis by hypertonic initiation block. J Virol. 1975 Jan;17(1):283–286. doi: 10.1128/jvi.17.1.283-286.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ostertag W., Crozier T., Kluge N., Melderis H., Dube S. Action of 5-bromodeoxyuridine on the induction of haemoglobin synthesis in mouse leukaemia cells resistant to 5-BUdR. Nat New Biol. 1973 Jun 13;243(128):203–205. doi: 10.1038/newbio243203a0. [DOI] [PubMed] [Google Scholar]
  15. Ostertag W., Melderis H., Steinheider G., Kluge N., Dube S. Synthesis of mouse haemoglobin and globin mRNA in leukaemic cell cultures. Nat New Biol. 1972 Oct 25;239(95):231–234. doi: 10.1038/newbio239231a0. [DOI] [PubMed] [Google Scholar]
  16. Ostertag W., Roesler G., Krieg C. J., Kind J., Cole T., Crozier T., Gaedicke G., Steinheider G., Kluge N., Dube S. Induction of endogenous virus and of thymidine kinase by bromodeoxyuridine in cell cultures transformed by Friend virus. Proc Natl Acad Sci U S A. 1974 Dec;71(12):4980–4985. doi: 10.1073/pnas.71.12.4980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Robertson D. L., Yau P., Dobbertin D. C., Sweeney T. K., Thach S. S., Brendler T., Thach R. E. Relationships between intracisternal type A and extracellular oncornavirus-like particles produced in murine MOPC-460 myeloma cells. J Virol. 1976 Apr;18(1):344–355. doi: 10.1128/jvi.18.1.344-355.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sato T., Friend C., De Harven E. Ultrastructural changes in Friend erythroleukemia cells treated with dimethyl sulfoxide. Cancer Res. 1971 Oct;31(10):1402–1417. [PubMed] [Google Scholar]
  19. Segrest J. P., Jackson R. L., Andrews E. P., Marchesi V. T. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis. Biochem Biophys Res Commun. 1971 Jul 16;44(2):390–395. doi: 10.1016/0006-291x(71)90612-7. [DOI] [PubMed] [Google Scholar]
  20. Shatkin A. J., Both G. W. Reovirus mRNA: transcription and translation. Cell. 1976 Mar;7(3):305–313. doi: 10.1016/0092-8674(76)90159-8. [DOI] [PubMed] [Google Scholar]
  21. Steeves R. A., Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein. J Virol. 1974 Jul;14(1):187–189. doi: 10.1128/jvi.14.1.187-189.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ussery M. A., Ramirez-Mitchell R., Hardesty B. A. Inhibition of friend murine leukemia virus production by low-ionic-strength medium. J Virol. 1976 Feb;17(2):453–461. doi: 10.1128/jvi.17.2.453-461.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Vogt V. M., Eisenman R. Identification of a large polypeptide precursor of avian oncornavirus proteins. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734–1738. doi: 10.1073/pnas.70.6.1734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Zaanie D., Gielkens A. L., Dekker-michielsen M. J., Bloemers H. P. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus. Virology. 1975 Oct;67(2):544–552. doi: 10.1016/0042-6822(75)90454-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES