Abstract
During infection of BS-C-1 cells (a permissive line of monkey kidney cells) with simian virus 40, there was a loss of infecting viral supercoiled (component I) DNA molecules which was partially inhibited by cycloheximide. The kinetics of loss of component I in the presence of cycloheximide suggested that the loss was the result of at least two activities acting on the DNA, one cycloheximide sensitive and the second cycloheximide insensitive. Nucleotides from degraded infecting viral DNA were reincorporated into cell DNA that was synthesized during the infection.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barbanti-Brodano G., Swetly P., Koprowski H. Early events in the infection of permissive cells with simian virus 40: adsorption, penetration, and uncoating. J Virol. 1970 Jul;6(1):78–86. doi: 10.1128/jvi.6.1.78-86.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Burlingham B. T., Doerfler W. An endonuclease in cells infected with adenovirus and associated with adenovirions. Virology. 1972 Apr;48(1):1–13. doi: 10.1016/0042-6822(72)90108-0. [DOI] [PubMed] [Google Scholar]
- Burlingham B. T., Doerfler W. Three size-classes of intracellular adenovirus deoxyribonucleic acid. J Virol. 1971 Jun;7(6):707–719. doi: 10.1128/jvi.7.6.707-719.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Collins C. J., Sauer G. Fate of infecting simian virus 40-deoxyribonucleic acid in nonpermissive cells: integration into host deoxyribonucleic acid. J Virol. 1972 Sep;10(3):425–432. doi: 10.1128/jvi.10.3.425-432.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Lavi S., Winocour E. Acquisition of sequences homologous to host deoxyribonucleic acid by closed circular simian virus 40 deoxyribonucleic acid. J Virol. 1972 Feb;9(2):309–316. doi: 10.1128/jvi.9.2.309-316.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zur Hausen H., Sokol F. Fate of adenovirus type 12 genomes in nonpermissive cells. J Virol. 1969 Sep;4(3):256–263. doi: 10.1128/jvi.4.3.256-263.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]