Abstract
Radiolabeled respiratory syncytial virus was purified from medium that had been harvested from infected HeLa cell monolayers before it contained much cellular debris. After isopycnic centrifugation in linear gradients prepared with sucrose dissolved in Hanks balanced salt solution, almost all the infectivity and most of the radioactivity were recovered in a single band with density from 1.16 to 1.23 g/cm3 and a peak at 1.2 g/cm3. Analysis by polyacrylamide gel electrophoresis resolved the purified virus into seven polypeptides of approximate molecular weights 20,000 to 80,000, of which the two largest and the smallest proved to by glycoproteins.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Berthiaume L., Joncas J., Pavilanis V. Comparative structure, morphogenesis and biological characteristics of the respiratory syncytial (RS) virus and the pneumonia virus of mice (PVM). Arch Gesamte Virusforsch. 1974;45(1-2):39–51. doi: 10.1007/BF01240540. [DOI] [PubMed] [Google Scholar]
- Hall W. W., Martin S. J. Purification and characterization of measles virus. J Gen Virol. 1973 May;19(2):175–188. doi: 10.1099/0022-1317-19-2-175. [DOI] [PubMed] [Google Scholar]
- Iinuma M., Simpson R. W. The structural proteins of Newcastle disease virus. I. Identification of a minor internal protein. Virology. 1974 Sep;61(1):229–239. doi: 10.1016/0042-6822(74)90257-8. [DOI] [PubMed] [Google Scholar]
- Jensik S. C., Silver S. Polypeptides of mumps virus. J Virol. 1976 Feb;17(2):363–373. doi: 10.1128/jvi.17.2.363-373.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levine S., Buthala D. A., Hamilton R. D. Late stage synchronization of respiratory syncytial virus replication. Virology. 1971 Aug;45(2):390–400. doi: 10.1016/0042-6822(71)90340-0. [DOI] [PubMed] [Google Scholar]
- Levine S., Hamilton R. Kinetics of the respiratory syncytial virus growth cycle in HeLa cells. Arch Gesamte Virusforsch. 1969;28(2):122–132. doi: 10.1007/BF01249378. [DOI] [PubMed] [Google Scholar]
- Mountcastle W. E., Compans R. W., Choppin P. W. Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1971 Jan;7(1):47–52. doi: 10.1128/jvi.7.1.47-52.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Norrby E., Marusyk H., Orvell C. Morphogenesis of respiratory syncytial virus in a green monkey kidney cell line (Vero). J Virol. 1970 Aug;6(2):237–242. doi: 10.1128/jvi.6.2.237-242.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Waters D. J., Bussell R. H. Polypeptide composition of measles and canine distemper viruses. Virology. 1973 Oct;55(2):554–557. doi: 10.1016/0042-6822(73)90202-x. [DOI] [PubMed] [Google Scholar]
- Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]