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. 1977 Feb;21(2):516–521. doi: 10.1128/jvi.21.2.516-521.1977

Homology between double-stranded RNA and nuclear DNA of yeast.

M Vodkin
PMCID: PMC353852  PMID: 319254

Abstract

The relationship between mycoviral double-stranded (ds) RNA and host cell DNA was investigated. Radiolabeled ds RNA was denatured and reannealed in the presence and absence of denatured DNA. RNA from killer strains of the yeast Saccharomyces cerevisiae and from nonkiller derivatives was utilized. The above-mentioned strains, as well as one that lacks all ds RNA, were sources for extracted DNA. Net hybridization of ds RNA to DNA occurred regardless of the strains from which the respective nucleic acids were prepared.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bevan E. A., Herring A. J., Mitchell D. J. Preliminary characterization of two species of dsRNA in yeast and their relationship to the "killer" character. Nature. 1973 Sep 14;245(5420):81–86. doi: 10.1038/245081b0. [DOI] [PubMed] [Google Scholar]
  2. Carter W. A., De Clercq E. Viral infection and host defense. Science. 1974 Dec 27;186(4170):1172–1178. doi: 10.1126/science.186.4170.1172. [DOI] [PubMed] [Google Scholar]
  3. Clark-Walker G. D., Miklos G. L. Localization and quantification of circular DNA in yeast. Eur J Biochem. 1974 Jan 16;41(2):359–365. doi: 10.1111/j.1432-1033.1974.tb03278.x. [DOI] [PubMed] [Google Scholar]
  4. Fink G. R., Styles C. A. Curing of a killer factor in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2846–2849. doi: 10.1073/pnas.69.10.2846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedrich R., Feix G. RNA-RNA hybridization in aqueous solutions containing formamide. Anal Biochem. 1972 Dec;50(2):467–476. doi: 10.1016/0003-2697(72)90056-5. [DOI] [PubMed] [Google Scholar]
  6. Gillespie D., Gallo R. C. RNA processing and RNA tumor virus origin and evolution. Science. 1975 May 23;188(4190):802–811. doi: 10.1126/science.47650. [DOI] [PubMed] [Google Scholar]
  7. Goldring E. S., Grossman L. I., Krupnick D., Cryer D. R., Marmur J. The petite mutation in yeast. Loss of mitochondrial deoxyribonucleic acid during induction of petites with ethidium bromide. J Mol Biol. 1970 Sep 14;52(2):323–335. doi: 10.1016/0022-2836(70)90033-1. [DOI] [PubMed] [Google Scholar]
  8. Herring A. J., Bevan E. A. Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. J Gen Virol. 1974 Mar;22(3):387–394. doi: 10.1099/0022-1317-22-3-387. [DOI] [PubMed] [Google Scholar]
  9. Lemke P. A., Nash C. H. Fungal viruses. Bacteriol Rev. 1974 Mar;38(1):29–56. doi: 10.1128/br.38.1.29-56.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Pays E. Double-stranded RNA in chromatin transcripts formed by exogenous RNA polymerase. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1121–1125. doi: 10.1073/pnas.73.4.1121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Rasse-Messenguy F., Fink G. R. Temperature-sensitive nonsense suppressors in yeast. Genetics. 1973 Nov;75(3):459–464. doi: 10.1093/genetics/75.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Robertson H. D., Webster R. E., Zinder N. D. Purification and properties of ribonuclease III from Escherichia coli. J Biol Chem. 1968 Jan 10;243(1):82–91. [PubMed] [Google Scholar]
  13. Rubin G. M. Preparation of RNA and ribosomes from yeast. Methods Cell Biol. 1975;12:45–64. doi: 10.1016/s0091-679x(08)60951-6. [DOI] [PubMed] [Google Scholar]
  14. Shalitin C., Fischer I. Abundant species of poly(A)-containing RNA from Saccharomyces cerevisiae. Biochim Biophys Acta. 1975 Dec 19;414(3):263–272. doi: 10.1016/0005-2787(75)90165-3. [DOI] [PubMed] [Google Scholar]
  15. Stern R., Friedman R. M. Ribonucleic acid synthesis in animal cells in the presence of actinomycin. Biochemistry. 1971 Sep 28;10(20):3635–3645. doi: 10.1021/bi00796a001. [DOI] [PubMed] [Google Scholar]
  16. Vodkin M. H., Fink G. R. A nucleic acid associated with a killer strain of yeast. Proc Natl Acad Sci U S A. 1973 Apr;70(4):1069–1072. doi: 10.1073/pnas.70.4.1069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Vodkin M., Katterman F., Fink G. R. Yeast killer mutants with altered double-stranded ribonucleic acid. J Bacteriol. 1974 Feb;117(2):681–686. doi: 10.1128/jb.117.2.681-686.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wickner R. B. "Killer character" of Saccharomyces cerevisiae: curing by growth at elevated temperature. J Bacteriol. 1974 Mar;117(3):1356–1357. doi: 10.1128/jb.117.3.1356-1357.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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