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. 1977 Feb;21(2):541–547. doi: 10.1128/jvi.21.2.541-547.1977

Variations in integration site of avian oncornaviruses in different hosts.

M N Dastoor, M Shoyab, M A Baluda
PMCID: PMC353855  PMID: 189085

Abstract

We examined the integration site of avian oncornaviruses in the genome of different hosts with respect to the repetitive frequency of the cellular DNA sequences adjacent to the integrated proviral DNA. The following systems were studied: avian sarcoma virus (B-77) and avian leukosis virus (Rous-associated virus-61) in cultured duck embryonic cells and B-77 in cultured mouse 3T3 cells. These systems represent different host responses to viral infection, i.e., one in which both cellular transformation and viral replication occur (B-77-infected duck cells), one in which viral replication, but not transformation, occurs (Rous-associated virus-61-infected duck cells), and one in which transformation, but not viral replication, occurs (B-77-infected 3T3 cells). Two sequential hybridizations were used. First, large denatured DNA fragments (2.8 X 10(6) daltons) were reassociated to different C0t (mole-seconds per liter) values. Next, DNA remaining single stranded at different C0t values was isolated by hydroxylapatite column chromatography, immobilized on nitrocellulose filters, and hybridized with an excess of 3H-labeled 35S viral RNA to titrate the concentration of proviral DNA. Results show that B-77 sarcoma virus and Rous-associated virus-61 integrate in the unique region of duck DNA, whereas B-77 proviral DNA is associated with both repeated and unique host DNA sequences in transformed mouse 3T3 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali M., Baluda M. A. Synthesis of avian oncornavirus DNA in infected chicken cells. J Virol. 1974 May;13(5):1005–1013. doi: 10.1128/jvi.13.5.1005-1013.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baluda M. A., Nayak D. P. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969 Nov;4(5):554–566. doi: 10.1128/jvi.4.5.554-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  5. Davidson E. H., Hough B. R., Amenson C. S., Britten R. J. General interspersion of repetitive with non-repetitive sequence elements in the DNA of Xenopus. J Mol Biol. 1973 Jun 15;77(1):1–23. doi: 10.1016/0022-2836(73)90359-8. [DOI] [PubMed] [Google Scholar]
  6. Davidson E. H., Hough B. R., Klein W. H., Britten R. J. Structural genes adjacent to interspersed repetitive DNA sequences. Cell. 1975 Mar;4(3):217–238. doi: 10.1016/0092-8674(75)90170-1. [DOI] [PubMed] [Google Scholar]
  7. Evans R. M., Baluda M. A., Shoyab M. Differences between the integration of avian myeloblastosis virus DNA in leukemic cells and of endogenous viral DNA in normal chicken cells. Proc Natl Acad Sci U S A. 1974 Aug;71(8):3152–3156. doi: 10.1073/pnas.71.8.3152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Evans R. M., Shoyab M., Baluda M. A. Studies on characterization of the integration sites of avian RNA tumor virus-specific DNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1005–1007. doi: 10.1101/sqb.1974.039.01.115. [DOI] [PubMed] [Google Scholar]
  9. Faust C. H., Diggelmann H., Mach B. Estimation of the number of genes coding for the constant part of the mouse immunoglobulin kappa light chain. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2491–2495. doi: 10.1073/pnas.71.6.2491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  11. Manning J. E., Schmid C. W., Davidson N. Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome. Cell. 1975 Feb;4(2):141–155. doi: 10.1016/0092-8674(75)90121-x. [DOI] [PubMed] [Google Scholar]
  12. Markham P. D., Baluda M. A. Integrated state of oncornavirus DNA in normal chicken cells and in cells transformed by avian myeloblastosis virus. J Virol. 1973 Oct;12(4):721–732. doi: 10.1128/jvi.12.4.721-732.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martin R. G., Chou J. Y., Avila J., Saral R. The semiautonomous replicon: a molecular model for the oncogenicity of SV40. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):17–24. doi: 10.1101/sqb.1974.039.01.005. [DOI] [PubMed] [Google Scholar]
  14. Pays E., Ronsse A. Interspersion of repetitive sequences in rat liver DNA. Biochem Biophys Res Commun. 1975 Feb 17;62(4):862–867. doi: 10.1016/0006-291x(75)90402-7. [DOI] [PubMed] [Google Scholar]
  15. Robinson W. S., Baluda M. A. The nucleic acid from avian myeloblastosis virus compared with the RNA from the Bryan strain of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1686–1692. doi: 10.1073/pnas.54.6.1686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shoyab M., Baluda M. A., Evans R. Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus. J Virol. 1974 Feb;13(2):331–339. doi: 10.1128/jvi.13.2.331-339.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shoyab M., Dastoor M. N., Baluda M. A. Evidence for tandem integration of avian myeloblastosis virus DNA with endogenous provirus in leukemic chicken cells. Proc Natl Acad Sci U S A. 1976 May;73(5):1749–1753. doi: 10.1073/pnas.73.5.1749. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Shoyab M., Evans R. M., Baluda M. A. Presence in leukemic cells of avian myeloblastosis virus-specific DNA sequences absent in normal chicken cells. J Virol. 1974 Jul;14(1):47–49. doi: 10.1128/jvi.14.1.47-49.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shoyab M., Markham P. D., Baluda M. A. Host induced alteration of avian sarcoma virus B-77 genome. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1031–1035. doi: 10.1073/pnas.72.3.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Temin H. M. The RNA tumor viruses--background and foreground. Proc Natl Acad Sci U S A. 1972 Apr;69(4):1016–1020. doi: 10.1073/pnas.69.4.1016. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Varmus H. E., Shank P. R. Unintegrated viral DNA is synthesized in the cytoplasm of avian sarcoma virus-transformed duck cells by viral DNA polymerase. J Virol. 1976 May;18(2):567–573. doi: 10.1128/jvi.18.2.567-573.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Varmus H. E., Vogt P. K., Bishop J. M. Integration of deoxyribonucleic acid specific for Rous sarcoma virus after infection of permissive and nonpermissive hosts. Proc Natl Acad Sci U S A. 1973 Nov;70(11):3067–3071. doi: 10.1073/pnas.70.11.3067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]

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