Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1978 Jan;25(1):86–96. doi: 10.1128/jvi.25.1.86-96.1978

Glycoproteins of avian tumor virus recombinants: evidence for intragenic crossing-over.

D M Galehouse, P H Duesberg
PMCID: PMC353904  PMID: 202758

Abstract

The envelope glycoproteins of several avian tumor virus recombinants selected for the host range of a leukosis virus and the transforming function of a sarcoma virus were compared with each other and with those of their parents. It was found that the glycoproteins of different recombinant viruses, derived from the same parents, differed in their electrophoretic mobilities measured in polyacrylmide gels. The glycoproteins that had lower electrophoretic mobilities had higher precentages of carbohydrate. The carbohydrate of viral glycoproteins was estimated to range between 8 and 18% from their buoyant densities in CsCl, using known glycoproteins as standards. After exhaustive Pronase digestion, the carbohydrate was recovered from viral glycoproteins as a mixture of glycopeptides with molecular weights ranging from 2,500 to 5,000. It was estimated that distinct viral glycoproteins contained between two and five such oligosaccharide chains and that the glycoproteins of different recombinants expressing the same host range marker may differ in the number of oligosaccharide chains and consequently also in their polypeptide structure. Those with lower electrophoretic mobility contain more oligosaccharide chains per molecule than those with higher electrophoretic mobilities. It is suggested that not oligosaccharide chains define the viral host range.

Full text

PDF
86

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
  2. Beemon K., Duesberg P., Vogt P. Evidence for crossing-over between avian tumor viruses based on analysis of viral RNAs. Proc Natl Acad Sci U S A. 1974 Oct;71(10):4254–4258. doi: 10.1073/pnas.71.10.4254. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bolognesi D. P., Bauer H., Gelderblom H., Hüper G. Polypeptides of avian RNA tumor viruses. IV. Components of the viral envelope. Virology. 1972 Mar;47(3):551–566. doi: 10.1016/0042-6822(72)90545-4. [DOI] [PubMed] [Google Scholar]
  4. Burge B. W., Huang A. S. Comparison of membrane protein glycopeptides of Sindbis virus and vesicular stomatitis virus. J Virol. 1970 Aug;6(2):176–182. doi: 10.1128/jvi.6.2.176-182.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burge B. W., Strauss J. H., Jr Glycopeptides of the membrane glycoprotein of Sindbis virus. J Mol Biol. 1970 Feb 14;47(3):449–466. doi: 10.1016/0022-2836(70)90314-1. [DOI] [PubMed] [Google Scholar]
  6. Duesberg P. H., Martin G. S., Vogt P. K. Glycoprotein components of avian and murine RNA tumor viruses. Virology. 1970 Aug;41(4):631–646. doi: 10.1016/0042-6822(70)90428-9. [DOI] [PubMed] [Google Scholar]
  7. Duesberg P. H., Robinson H. L., Robinson W. S., Huebner R. J., Turner H. C. Proteins of Rous sarcoma virus. Virology. 1968 Sep;36(1):73–86. doi: 10.1016/0042-6822(68)90118-9. [DOI] [PubMed] [Google Scholar]
  8. Duesberg P. H., Vogt P. K. RNA species obtained from clonal lines of avian sarcoma and from avian leukosis virus. Virology. 1973 Jul;54(1):207–219. doi: 10.1016/0042-6822(73)90130-x. [DOI] [PubMed] [Google Scholar]
  9. Duesberg P., Helm K. V., Canaani E. Properties of a soluble DNA polymerase isolated from Rous sarcoma virus. Proc Natl Acad Sci U S A. 1971 Apr;68(4):747–751. doi: 10.1073/pnas.68.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Duesberg P., Vogt P. K., Beemon K., Lai M. Avian RNA tumor viruses: mechanism of recombination and complexity of the genome. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):847–857. doi: 10.1101/sqb.1974.039.01.099. [DOI] [PubMed] [Google Scholar]
  11. Etchison J. R., Holland J. J. Carbohydrate composition of the membrane glycoprotein of vesicular stomatitis virus. Virology. 1974 Jul;60(1):217–229. doi: 10.1016/0042-6822(74)90379-1. [DOI] [PubMed] [Google Scholar]
  12. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Grimes W. J., Burge B. W. Modification of Sindbis virus glycoprotein by host-specified glycosyl transferases. J Virol. 1971 Mar;7(3):309–313. doi: 10.1128/jvi.7.3.309-313.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ishizaki R., Vogt P. K. Immunological relationships among envelope antigens of avian tumor viruses. Virology. 1966 Nov;30(3):375–387. doi: 10.1016/0042-6822(66)90116-4. [DOI] [PubMed] [Google Scholar]
  15. Kawai S., Hanafusa H. Genetic recombination with avian tumor virus. Virology. 1972 Jul;49(1):37–44. doi: 10.1016/s0042-6822(72)80005-9. [DOI] [PubMed] [Google Scholar]
  16. Krantz M. J., Lee Y. C., Hung P. P. Carbohydrate groups in the major glycoprotein of Rous sarcoma virus. Nature. 1974 Apr 19;248(5450):684–686. doi: 10.1038/248684a0. [DOI] [PubMed] [Google Scholar]
  17. Krantz M. J., Lee Y. C., Hung P. P. Characterization and comparison of the major glycoprotein from three strains of Rous sarcoma virus. Arch Biochem Biophys. 1976 May;174(1):66–73. doi: 10.1016/0003-9861(76)90324-6. [DOI] [PubMed] [Google Scholar]
  18. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  19. Lai M. M., Duesberg P. H. Differences between the envelope glycoproteins and glycopeptides of avian tumor viruses released from transformed and from nontransformed cells. Virology. 1972 Nov;50(2):359–372. doi: 10.1016/0042-6822(72)90387-x. [DOI] [PubMed] [Google Scholar]
  20. Rohrschneider L., Bauer H., Bolognesi D. P. Group-specific antigenic determinants of the large envelope glycoprotein of avian oncornaviruses. Virology. 1975 Sep;67(1):234–241. doi: 10.1016/0042-6822(75)90420-1. [DOI] [PubMed] [Google Scholar]
  21. Scheele C. M., Hanafusa H. Proteins of helper-dependent RSV. Virology. 1971 Aug;45(2):401–410. doi: 10.1016/0042-6822(71)90341-2. [DOI] [PubMed] [Google Scholar]
  22. Segrest J. P., Jackson R. L., Andrews E. P., Marchesi V. T. Human erythrocyte membrane glycoprotein: a re-evaluation of the molecular weight as determined by SDS polyacrylamide gel electrophoresis. Biochem Biophys Res Commun. 1971 Jul 16;44(2):390–395. doi: 10.1016/0006-291x(71)90612-7. [DOI] [PubMed] [Google Scholar]
  23. VOGT P. K. A HETEROGENEITY OF ROUS SARCOMA VIRUS REVEALED BY SELECTIVELY RESISTANT CHICK EMBRYO CELLS. Virology. 1965 Feb;25:237–247. doi: 10.1016/0042-6822(65)90202-3. [DOI] [PubMed] [Google Scholar]
  24. Vogt P. K. Genetically stable reassortment of markers during mixed infection with avian tumor viruses. Virology. 1971 Dec;46(3):947–952. doi: 10.1016/0042-6822(71)90093-6. [DOI] [PubMed] [Google Scholar]
  25. Vogt P. K., Ishizaki R. Patterns of viral interference in the avian leukosis and sarcoma complex. Virology. 1966 Nov;30(3):368–374. doi: 10.1016/0042-6822(66)90115-2. [DOI] [PubMed] [Google Scholar]
  26. Wang L. H., Duesberg P., Mellon P., Vogt P. K. Distribution of envelope-specific and sarcoma-specific nucleotide sequences from different parents in the RNAs of avian tumor virus recombinants. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1073–1077. doi: 10.1073/pnas.73.4.1073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES