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. 1978 Jan;25(1):298–304. doi: 10.1128/jvi.25.1.298-304.1978

Binding and transcription of simian virus 40 DNA by DNA-dependent RNA polymerase from Escherichia coli.

P Hale, J Lebowitz
PMCID: PMC353926  PMID: 202742

Abstract

Supercoiled simian virus 40 was transcribed more efficiently than nonsupercoiled DNA. The effect was increased from two- to fivefold by the addition of rifampin with triphosphates. The number and locations of polymerase binding sites with respect to Hin II-III restriction fragments were determined. The total number of binding sites was nine, as determined by UV difference spectroscopy. The locations of these binding sites were on the A, B, D, E, F, and G fragments, as determined by gel electrophoresis. The number of sites was the same for both supercoiled and relaxed or Hin II-III-digested DNA, and the point of saturation of supercoiled DNA by polymerase remained the same with increasing concentrations of rifampin from 0 to 8 microgram/ml.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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