Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1978 Jan;25(1):374–383. doi: 10.1128/jvi.25.1.374-383.1978

Synthesis and processing of precursor polypeptides to murine mammary tumor virus structural proteins.

J Racevskis, N H Sarkar
PMCID: PMC353935  PMID: 202748

Abstract

Biosynthesis of murine mammary tumor virus (MuMTV) proteins was studied in the chronically MuMTV-infected epithelial cell line MuMT-73 by using monospecific antisera to the major MuMTV core protein p27 and the major envelope glycoprotein gp47. In pulse-labeling experiments using [35S]methionine, monospecific antisera to p27 precipitated a 75,000-molecular-weight protein as the major intracellular component. Analysis of the same cellular extracts with monospecific antisera to gp47 revealed that the gp47 precursor was a 70,000-dalton protein. After chase periods, there was a loss of label from the precursors and a concomitant increase of labeled extracellular mature viral proteins. The glycoprotein precursor incorporated labeled glucosamine and seemed to be processed more rapidly than the p27 precursor. Considerable amounts of apparently nonvirion-associated gp47 and glycoprotein precursor could be detected in the extracellular culture fluid.

Full text

PDF
374

Images in this article

376Image
on p.376
377Image
on p.377
378Image
on p.378
379Image
on p.379
380Image
on p.380
381Image
on p.381

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. Tumor viruses: 1974. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):1187–1200. doi: 10.1101/sqb.1974.039.01.137. [DOI] [PubMed] [Google Scholar]
  2. Clarke M., Spudich J. A. Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu Rev Biochem. 1977;46:797–822. doi: 10.1146/annurev.bi.46.070177.004053. [DOI] [PubMed] [Google Scholar]
  3. Dickson C., Puma J. P., Nandi S. Identification of a precursor protein to the major glycoproteins of mouse mammary tumor virus. J Virol. 1975 Jan;17(1):275–282. doi: 10.1128/jvi.17.1.275-282.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dickson C., Puma J. P., Nandi S. Intracellular synthesis of mouse mammary tumor virus polypeptides: indication of a precursor glycoprotein. J Virol. 1975 Aug;16(2):250–258. doi: 10.1128/jvi.16.2.250-258.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dickson C., Skehel J. J. The polypeptide composition of mouse mammary tumor virus. Virology. 1974 Apr;58(2):387–395. doi: 10.1016/0042-6822(74)90074-9. [DOI] [PubMed] [Google Scholar]
  6. Hynes R. O. Cell surface proteins and malignant transformation. Biochim Biophys Acta. 1976 Apr 30;458(1):73–107. doi: 10.1016/0304-419x(76)90015-9. [DOI] [PubMed] [Google Scholar]
  7. Hynes R. O., Destree A. Extensive disulfide bonding at the mammalian cell surface. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2855–2859. doi: 10.1073/pnas.74.7.2855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Jamjoom G. A., Naso R. B., Arlinghaus R. B. Further characterization of intracellular precursor polyproteins of Rauscher leukemia virus. Virology. 1977 May 1;78(1):11–34. doi: 10.1016/0042-6822(77)90075-7. [DOI] [PubMed] [Google Scholar]
  9. Kessler S. W. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975 Dec;115(6):1617–1624. [PubMed] [Google Scholar]
  10. Kimball P. C., Boehm-Truitt M., Schochetman G., Schlom J. Characterization of mouse mammary tumor viruses from primary tumor cell cultures.I. Immunologic and structural studies. J Natl Cancer Inst. 1976 Jan;56(1):111–117. doi: 10.1093/jnci/56.1.111. [DOI] [PubMed] [Google Scholar]
  11. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  12. Lasfargues E. Y., Kramarsky B., Sarkar N. H., Lasfargues J. C., Moore D. H. An established R3 mouse mammary tumor cell line; kinetics of mammary tumor virus (MTV) production. Proc Soc Exp Biol Med. 1972 Jan;139(1):242–247. doi: 10.3181/00379727-139-36119. [DOI] [PubMed] [Google Scholar]
  13. Marcus S. L., Sarkar N. H., Modak M. J. Purification and properties of murine mammary tumor virus DNA polymerase. Virology. 1976 May;71(1):242–254. doi: 10.1016/0042-6822(76)90109-4. [DOI] [PubMed] [Google Scholar]
  14. Owens R. B., Hackett A. J. Tissue culture studies of mouse mammary tumor cells and associated viruses. J Natl Cancer Inst. 1972 Nov;49(5):1321–1332. [PubMed] [Google Scholar]
  15. Racevskis J., Koch G. Viral protein synthesis in Friend erythroleukemia cell lines. J Virol. 1977 Jan;21(1):328–337. doi: 10.1128/jvi.21.1.328-337.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ritzi E., Martin D. S., Stolfi R. L., Spiegelman S. Plasma levels of a viral protein as a diagnostic signal for the presence of tumor : the murine mammary tumor model. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4190–4194. doi: 10.1073/pnas.73.11.4190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sarkar N. H., Dion A. S. Polypeptides of the mouse mammary tumor virus. I. Characterization of two group-specific antigens. Virology. 1975 Apr;64(2):471–491. doi: 10.1016/0042-6822(75)90125-7. [DOI] [PubMed] [Google Scholar]
  18. Sarkar N. H., Pomenti A. A., Dion A. S. Replication of mouse mammary tumor virus in tissue culture. 1. Establishment of a mouse mammary tumor cell line, virus characterization, and quantitation of virus production. Virology. 1977 Mar;77(1):12–30. doi: 10.1016/0042-6822(77)90402-0. [DOI] [PubMed] [Google Scholar]
  19. Sarkar N. H., Taraschi N. E., Pomenti A. A., Dion A. S. Polypeptides of the mouse mammary tumor virus. II. Identification of two major glycoproteins with the viral structure. Virology. 1976 Feb;69(2):677–690. doi: 10.1016/0042-6822(76)90496-7. [DOI] [PubMed] [Google Scholar]
  20. Sarkar N. H., Whittington E. S. Identification of the structural proteins of the murine mammary tumor virus that are serologically related to the antigens of intracytoplasmic type-A particles. Virology. 1977 Aug;81(1):91–106. doi: 10.1016/0042-6822(77)90061-7. [DOI] [PubMed] [Google Scholar]
  21. Schochetman G., Schlom J. Independent polypeptide chain initiation sites for the synthesis of different classes of proteins for an RNA tumor virus: mouse mammary tumor virus. Virology. 1976 Sep;73(2):431–441. doi: 10.1016/0042-6822(76)90404-9. [DOI] [PubMed] [Google Scholar]
  22. Shapiro S. Z., August T. The use of immunoprecipitation to study the synthesis and cleavage processing of viral proteins. J Immunol Methods. 1976;13(2):153–159. doi: 10.1016/0022-1759(76)90153-8. [DOI] [PubMed] [Google Scholar]
  23. Shapiro S. Z., Strand M., August J. T. High molecular weight precursor polypeptides to structural proteins of Rauscher murine leukemia virus. J Mol Biol. 1976 Nov 15;107(4):459–477. doi: 10.1016/s0022-2836(76)80078-2. [DOI] [PubMed] [Google Scholar]
  24. Teramoto Y. A., Puentes M. J., Young L. J., Cardiff R. D. Structure of the mouse mammary tumor virus: polypeptides and glycoproteins. J Virol. 1974 Feb;13(2):411–418. doi: 10.1128/jvi.13.2.411-418.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Van Blerkom J., Manes C. Development of preimplantation rabbit embryos in vivo and in vitro. Dev Biol. 1974 Sep;40(1):40–51. doi: 10.1016/0012-1606(74)90105-5. [DOI] [PubMed] [Google Scholar]
  26. Van Zaane D., Dekker-Michielsen J. A., Bloemers H. P. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus: synthesis, identification, and processing. Virology. 1976 Nov;75(1):113–129. doi: 10.1016/0042-6822(76)90011-8. [DOI] [PubMed] [Google Scholar]
  27. Yagi M. J., Compans R. W. Structural components of mouse mammary tumor virus. I. Polypeptides of the virion. Virology. 1977 Feb;76(2):751–766. doi: 10.1016/0042-6822(77)90256-2. [DOI] [PubMed] [Google Scholar]
  28. Yagi M. J. Cultivation and characterization of BALB-cfC3H mammary tumor cell lines. J Natl Cancer Inst. 1973 Dec;51(6):1849–1860. doi: 10.1093/jnci/51.6.1849. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES