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. 2013 Jan 3;3:484. doi: 10.3389/fphys.2012.00484

Effects of angiotensin II on the cerebral circulation: role of oxidative stress

T Michael De Silva 1, Frank M Faraci 1,2,*
PMCID: PMC3539653  PMID: 23316164

Abstract

Oxidative stress has emerged as a key component of many diseases that affect the vasculature. Oxidative stress is characterized as a cellular environment where the generation of oxidant molecules overwhelms endogenous anti-oxidant defense mechanisms. NADPH oxidases are a family of enzymes whose primary purpose is generation of reactive oxygen species (oxidant molecules) and therefore are likely to be key contributors to oxidative stress. Hypertension is associated with oxidative stress in the vasculature and is a major risk factor for stroke and cognitive abnormalities. Angiotensin II (Ang II) is the main effector peptide of the renin-angiotensin system (RAS) and plays a critical role in promoting oxidative stress in the vasculature. In the cerebral circulation, Ang II has been implicated in reactive oxygen species generation, alterations to vasomotor function, impaired neurovascular coupling, inflammation, and vascular remodeling. Furthermore, studies in humans have shown that cerebral blood flow is altered during hypertension and therapeutically targeting the RAS improves cerebral blood flow. Importantly, many of the aforementioned effects have been shown to be dependent on NADPH oxidases. Thus, Ang II, NADPH oxidases and oxidative stress are likely to play key roles in the pathogenesis of hypertension and associated cerebrovascular disease. This review will focus on our current understanding of the contribution of Ang II and NADPH oxidases to oxidative stress in the cerebral circulation.

Keywords: cerebral blood flow, endothelium, cerebral arteries, NADPH oxidase, nitric oxide, neurovascular coupling

Introduction

Hypertension is a highly prevalent disease throughout the world. For example, it is currently estimated that 1 in 3 adults in the United States has high blood pressure, which is defined as having systolic blood pressure ≥140 mmHg and/or diastolic blood pressure ≥90 mmHg, or being on medication to control their blood pressure (Roger et al., 2011). Hypertension has profound effects on the cerebral circulation and the brain and is a major risk factor for both ischemic and hemorrhagic stroke as well as cognitive abnormalities (Kivipelto et al., 2001; Lewington et al., 2002; O'Donnell et al., 2010). Angiotensin II (Ang II) is a pleiotropic peptide that has multiple effects on the cerebral vasculature. Importantly, Ang II plays a critical role in the pathogenesis of hypertension. Two of the most effective anti-hypertensive therapies, angiotensin converting enzyme (ACE) inhibitors and angiotensin type 1 (AT1) receptor antagonists, target key elements of the renin-angiotensin system (RAS). It has also been established that hypertension causes oxidative stress within the vasculature and Ang II is a key contributor to this effect. Within the cerebral circulation, Ang II stimulates the generation of reactive oxygen species (ROS) and has effects on regulation of vascular tone as well as promoting inflammation and changes to vascular structure. All of these effects can influence cerebral blood flow. Either alone or in combination with other mechanisms, Ang II may underline many of the deleterious effects of hypertension on the cerebral vasculature and brain.

In the present review, we will consider recent evidence implicating Ang II as a cause of oxidative stress specifically in the cerebral circulation. As changes in cerebrovascular function may ultimately impact cerebral blood flow, studies examining the effects of hypertension on cerebral blood flow and the role of Ang II and oxidative stress will also be discussed.

What is oxidative stress?

Oxidative stress is characterized by a shift from a cellular environment where the production and metabolism of oxidant molecules are tightly controlled to one of elevated levels of the same molecules. The excess oxidant molecules may then overwhelm anti-oxidant defense systems, resulting in oxidative stress. Such a shift in balance can occur due to an overproduction of ROS, such as superoxide (O2·−), or a reduction in the removal of ROS by oxidant defense mechanisms. Key anti-oxidants include O2·− dismutases (SOD), glutathione peroxidases, and catalase. ROS can be generated by multiple enzymes within the vasculature as well as non-enzymatic sources. O2·− anion is the parent ROS molecule produced by the one electron reduction of molecular oxygen by various oxidases (e.g., NADPH oxidase, cyclooxygenase, enzymes in the mitochondrial electron transport chain, lipoxygenases, cytochrome P450 enzymes). O2·− can then be dismutated by SOD, resulting in the generation of hydrogen peroxide (H2O2; Figure 1). These ROS can undergo further enzymatic or non-enzymatic reactions to generate other powerful oxidant molecules, such as hydroxyl radical (OH·), peroxynitrite (ONOO−), and hyperchlorous acid (HOCl) (Chrissobolis and Faraci, 2008; Miller et al., 2010) (Figure 1).

Figure 1.

Figure 1

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Interactions between nitric oxide (NO·) and reactive oxygen species (ROS). NO· is a critical component of mechanisms that regulate cerebrovascular homeostasis. Diverse stimuli (including shear stress, neurotransmitters like acetylcholine and activation of neurons) can result in the generation of NO· by either endothelial or neuronal nitric oxide synthase (eNOS and nNOS, respectively). The generation of NO· is dependent on the presence of the substrate L-arginine (L-Arg) and enzyme co-factors including tetrahydrobiopterin (BH4). NO· activates its receptor in vascular muscle soluble guanylate cyclase (sGC), which results in the formation of cyclic guanosine monophosphate (cGMP). This results in numerous signaling events and functional effects which include cerebral vasodilation. NADPH oxidases (Nox1 and Nox2) generate superoxide (O2·−) and Nox4 can generate hydrogen peroxide (H2O2), ROS can then participate in signaling events, but may also cause cellular injury. O2·− is a potent scavenger of NO·, which reduces the bioavailability of NO· and results in the formation of peroxynitrite (ONOO−), which also causes cellular injury. H2O2 can react with heavy metals to form the highly reactive and toxic hydroxyl radical (OH·).

Oxidative stress has been implicated in the pathogenesis of many diseases that affect the cerebral circulation and the brain. It is a fundamental component of aging as well as many diseases including hypertension, hypercholesterolemia, atherosclerosis, diabetes, stroke, and Alzheimer's disease. One of the key consequences of oxidative stress is impaired signaling of the vasoprotective molecule nitric oxide (NO·), resulting in alterations in vascular function and structure in addition to other effects (Figure 1). Furthermore, oxidative stress contributes to inflammation as well as damage to proteins, DNA and other macromolecules. Thus, oxidative stress has wide implications for overall cerebrovascular function and regulation. As such, it is no surprise that there has been substantial effort devoted to understanding mechanisms that contribute to oxidative stress as well as therapies to reduce oxidative damage.

Many lines of evidence strongly indicate that oxidative stress has damaging effects on vascular function. However, production of ROS per se is not always detrimental as ROS sometimes have beneficial effects within the vasculature. Specifically, some ROS can function as vasodilators in cerebral arteries during physiological conditions (Didion and Faraci, 2002; Park et al., 2004; Miller et al., 2005, 2007a; Modrick et al., 2009) as well as during disease (Paravicini et al., 2004; Kitayama et al., 2007). So, while elevated levels of ROS contribute to oxidative stress including vascular damage and dysfunction, lower levels of ROS may be important signaling molecules for some vasoactive stimuli. Cerebral arteries have the capacity to generate significantly higher levels of ROS compared with systemic arteries (Miller et al., 2005, 2009). So, while ROS may serve as an important signaling molecules and vasodilators under physiological conditions, higher levels of ROS may underlie the pathogenesis of disease in the cerebral circulation. Therefore, to presumably avoid potentially harmful levels of ROS, the production and metabolism of ROS are typically tightly controlled.

The presence of oxidative stress has major implications for cerebrovascular function. Oxidative stress has been implicated in impaired cellular signaling, vascular remodeling, and inflammation. Perhaps the best characterized mechanisms by which oxidative stress can impair vascular function is via the disruption of endothelium-dependent NO· signaling. This mechanism involves the chemical reaction between O2·− and NO·, one of the fastest known biological reactions (Thomson et al., 1995), approximately three times faster than the dismutation of O2·− by SOD. Importantly, this reaction not only reduces the bioavailability of NO·, but also simultaneously generates the highly toxic reactive nitrogen species (RNS) ONOO−, which can have a number of damaging cellular effects (Figure 1). Thus, if levels are increased locally, O2·− will rapidly react with and reduce the bioavailability of NO·, resulting in oxidative stress. As NO· is a key mediator of cerebral vasodilation driven by both endothelium and neurons, alterations that reduce the bioavailability of NO· would impair these responses (Figure 1).

What are the sources of ROS that contribute to oxidative stress?

A number of enzymes within the vasculature can be sources of ROS, including xanthine oxidase (Kinugawa et al., 2005), cyclooxygenases (Didion et al., 2001; Niwa et al., 2001), uncoupled NOS (Vasquez-Vivar et al., 1997, 1998; Landmesser et al., 2003; Dikalova et al., 2010; Santhanam et al., 2012), and the mitochondrial electron transport chain (Narayanan et al., 2010). Several of these enzymes generate ROS as either a by-product of normal enzyme activity or when the protein is in a dysfunctional state. By contrast, NADPH oxidases are the only known family of enzymes whose primary function is the generation of ROS (Drummond et al., 2011). Thus, NADPH oxidases are candidates for deliberate ROS production required for normal signaling and for the excessive ROS associated with oxidative stress. It has also been suggested that NADPH oxidase may serve as a source of ROS that initiates a cascade of events including feed-forward mechanisms and recruitment of other enzymes that promote further oxidative stress (Faraci, 2006; Selemidis et al., 2008).

At present study, seven isoforms of NADPH oxidase have been identified. These enzymes are named Nox1, Nox2, Nox3, Nox4, and Nox5 containing NADPH oxidases and DUOX1 and DUOX2. In general, all NADPH oxidases generate O2·− by transferring electrons from the substrate, NADPH, via the Nox catalytic subunit to molecular oxygen. Nox1, Nox2, Nox4, and Nox5 containing isoforms are all expressed within vascular cells, however, to date only the Nox1, Nox2, and Nox4 containing isoforms have been identified in the cerebral vasculature (Ago et al., 2005; Miller et al., 2007a). Nox5 containing NADPH oxidase is not expressed in rats and mice and as such, very little is known about this isoform and nothing in regards to any potential role in cerebral arteries. Further studies, potentially including studies of genetic models expressing Nox5 in vascular cells are needed to determine any potential contribution of this isoform to oxidative stress in blood vessels supplying brain. Similarly, our understanding of the expression and contribution, if any, of DUOX1/2 to oxidative stress and vascular disease is extremely limited.

Many studies have implicated a role for NADPH oxidase-derived ROS in models of cerebrovascular disease. As such, the contribution of NADPH oxidases to Ang II-induced oxidative stress will be the focus of this review. However, it is important to remember that other oxidase enzymes and mechanisms may also contribute to oxidative stress during disease.

NADPH oxidases

Here, we will briefly discuss some of the key features of the three isoforms of NADPH oxidase that have been described within the cerebral vasculature. The detailed structural and regulatory differences between these enzymes have been discussed previously and will not be discussed again here (Bedard and Krause, 2007; Selemidis et al., 2008).

Structure of Nox1, Nox2, and Nox4 NADPH oxidase

The NADPH oxidase family of enzymes was discovered and initially studied in neutrophils, where Nox2 NADPH oxidase plays a role in immunological host defense. Subsequently, Nox1, Nox2, and Nox4 NADPH oxidases were identified in vascular cells where they are thought to play important roles under both physiological and pathophysiological conditions (Miller et al., 2006).

Nox2 NADPH oxidase consists of two membrane-bound subunits (Nox2 and p22phox), up to three cytosolic subunits [p47phox (organizer subunit), p67phox (activator subunit) and potentially p40phox] and the small G-protein Rac. The catalytic domain resides in Nox2, which contains all of the necessary components to facilitate the transfer of electrons from the substrate, NADPH to molecular oxygen. Specifically, the catalytic domain of Nox2 possesses two haem containing groups and a FAD group, as well as a binding site for NADPH (Taylor et al., 1993; Cheng et al., 2001). The p22phox subunit forms a heterodimer with Nox2 and contains a proline rich region that facilitates the binding of p47phox with the membrane bound subunits (Groemping et al., 2003). As such, p22phox plays a crucial role in regulating Nox2 activity (Ambasta et al., 2004). In unstimulated cells, Nox2 is dormant, and its activation requires the interaction of Nox2/p22phox with the regulatory cytosolic complex containing the p47phox and p67phox subunits. During unstimulated conditions, p47phox is not associated with the Nox2/p22phox complex due to the presence of an autoinhibitory domain which prevents the interaction of p47phox with the proline rich region of p22phox (Groemping et al., 2003). Translocation of p47phox to the membrane bound subunits is dependent on protein kinase C (PKC)-dependent phosphorylation of serine residues on p47phox (Fontayne et al., 2002), which results in a conformational change and displacement of the autoinhibitory region on p47phox (Groemping et al., 2003). p67phox binds to p47phox and this complex translocates to the membrane where two SH3 domains on p47phox bind to the proline rich region of p22phox (Groemping et al., 2003; Nobuhisa et al., 2006). In addition, p47phox has also been shown to bind multiple sites on Nox2, which is believed to be crucial for the final assembly of the active oxidase (Deleo et al., 1995). It has been suggested that the binding of Rac induces a conformation change in p67phox, which in turn promotes its binding to Nox2 (Sarfstein et al., 2004), which leads to a conformation change and electron flow through the Nox2 subunit (Nisimoto et al., 1999). Much less is known regarding the role of p40phox in the activation of Nox2 NADPH. However, in coronary endothelial cells isolated from p47phox-deficient mice, p40phox may act as an alternative organizer subunit in the absence of p47phox (Fan et al., 2009). Currently, it remains to be determined if p40phox is necessary for Nox2 NADPH oxidase activation in the cerebral circulation. The latter point notwithstanding, once the Nox2 NADPH oxidase enzyme complex is assembled, electrons can then be transferred from NADPH to FAD and then to the haem groups and finally to molecular oxygen, thus generating O2·−.

Nox1 NADPH oxidase is structurally similar to Nox2 NADPH oxidase, but appears to utilize different regulatory cytosolic subunits for its activation. Like the Nox2 subunit, the Nox1 subunit contains two haem groups, FAD and NADPH binding sites, and forms a heterodimer with p22phox in the cell membrane (Ambasta et al., 2004; Kawahara et al., 2005). Nox1 NADPH oxidase is activated by NoxO1 (Nox organizer 1) and NoxA1 (Nox activator 1), which are homologs of p47phox and p67phox, respectively (Banfi et al., 2003). While there is evidence that Nox1 NADPH may be activated by p47phox and p67phox (Banfi et al., 2003), the interaction of Nox1 with NoxO1/NoxA1 is more effective than its interaction with p46phox/p67phox (Banfi et al., 2003). It was recently shown that preventing Rac activation by inhibiting PI3-kinase only attenuates Nox1 NADPH oxidase activity by approximately 20% (Choi et al., 2008), suggesting that it may not be critical for Nox1 NADPH oxidase activity. Unlike p47phox, NoxO1 lacks an autoinhibitory domain and it has been hypothesized that the Nox1 NADPH oxidase might be basally active (Banfi et al., 2003). However, studies using Nox1 deficient mice suggest that Nox1 NADPH oxidase does not contribute to O2·− production under basal conditions in cerebral arteries (Jackman et al., 2009).

Nox4 NADPH oxidase was discovered in the kidney (originally named Renox) where it was proposed to serve as an oxygen sensing enzyme (Geiszt et al., 2000). Similar to Nox1 and Nox2 NADPH oxidase, the Nox4 catalytic domain contains all of the required components for oxidase activity and forms a heterodimer with p22phox (Ambasta et al., 2004; Martyn et al., 2006). However, one unique feature of Nox4 NADPH oxidase is that it may not require cytosolic subunits or Rac for oxidase activity and may be basally active (Martyn et al., 2006). Also, Nox4 NADPH oxidase may preferentially generate H2O2, rather than O2·− (Martyn et al., 2006; Schröder et al., 2012) (Figure 1).

Expression of cerebrovascular NADPH oxidase

Our understanding of the expression profile of NADPH oxidase isoforms in cerebral arteries lags behind that of systemic arteries. However, it has been revealed that mRNA for the Nox1, Nox2, and Nox4 catalytic subunits, p22phox as well as the cytosolic subunits p47phox, p67phox, NoxO1, and NoxA1, are expressed in the rat basilar artery (Paravicini et al., 2004; Ago et al., 2005). Furthermore, Nox1, Nox2, and Nox4 proteins are expressed in cerebral arteries (Miller et al., 2007a). The specific cellular localization of each Nox isoform is distinct. In the vasculature in general, it is believed that Nox1, Nox2, and Nox4 are expressed in endothelial cells, Nox1 and Nox4 are expressed in smooth muscle cells and Nox2 and Nox4 expressed in adventitia (Drummond et al., 2011). Indeed, using immunostaining, Nox1 was shown to be present in all layers of the basilar artery (Ago et al., 2005). Furthermore, using immunostaining and electron microscopy, it has also been demonstrated that Nox2 protein is primarily expressed in endothelial and adventitial cells of cerebral arteries (Kazama et al., 2004; De Silva et al., 2009; Miller et al., 2009). Nox4 immunostaining has been shown to be present throughout the vascular wall (Kleinschnitz et al., 2010).

Subcellular localization of NADPH oxidases in cerebral arteries

Very few studies have investigated the specific subcellular localization of the different Nox isoforms in cerebral arteries. In rats and mice, electron microscopy has been used to localize Nox2 to the plasma membrane of endothelial cells (Kazama et al., 2004). Furthermore, Nox2 immunoreactivity was also detected in the cytoplasm of endothelial cells (Kazama et al., 2004), although the exact subcellular structure where Nox2 was located was not determined. While we currently lack further detail regarding the intracellular localization of Nox isoforms in cerebral vessels, work in cultured vascular cells has revealed differences in the localization of the Nox isoforms. In cultured endothelial cells, diffuse perinuclear staining for Nox1 was observed (Sipkens et al., 2011), whereas in cultured vascular smooth muscle cells, Nox1 has been shown to be expressed at the plasma membrane, co-localized with caveolae (Hilenski et al., 2004). Furthermore, Nox1 is expressed in early endosomes in vascular smooth muscle cells (Miller et al., 2007b). Nox2 protein was found in lipid raft rich fractions from cultured endothelial cells (Zhang et al., 2006). Interestingly, in endothelial cells, there is both evidence for (Li and Shah, 2002) and against (Sipkens et al., 2011) perinuclear expression of Nox2. Finally, Nox4 has been identified in the nucleus (Hilenski et al., 2004; Kuroda et al., 2005) and the endoplasmic reticulum (Van Buul et al., 2005; Chen et al., 2008; Lyle et al., 2009) in vascular smooth muscle and endothelial cells. In addition, Nox4 has also been shown to be co-localized with focal adhesions in vascular smooth muscle (Hilenski et al., 2004; Lyle et al., 2009). While studies in cultured vascular cells are vital in our understanding of subcellular localization of the different isoforms of NADPH oxidase, it is important that these findings are confirmed in intact cerebral arteries.

Effects of Ang II on cerebrovascular function

Generation of reactive oxygen species

A well-known effect of exposure to Ang II in the vasculature is increased generation of ROS. Acute Ang II treatment increases ROS production by cerebral arteries from mice (Kazama et al., 2004; De Silva et al., 2009; Jackman et al., 2009) and rats (Miller et al., 2005). Furthermore, both Nox1 (Jackman et al., 2009) and Nox2 (Kazama et al., 2004; De Silva et al., 2009) contribute to Ang II stimulated ROS production as genetic deletion of the catalytic subunit of either of these isoforms reduces Ang II stimulated ROS production by cerebral arteries. In this regard, it is interesting to note that in non-cerebral arteries from Nox1-deficient mice, AT1 receptors fail to properly localize at the cell membrane (Basset et al., 2009), suggesting that Nox1-derived ROS may regulate expression of AT1 receptors at the cell surface. While it remains to be established if this same pattern occurs in cerebral vessels, it is conceivable that the attenuation in Ang II-stimulated O2·− production found in cerebral arteries from Nox1-deficient mice may actually be due to impaired trafficking of the AT1 receptor to the cell membrane. The importance of Nox4 in generating ROS in response to Ang II in cerebral arteries is currently unknown. In addition to rapid effects in vitro, acute systemic administration of Ang II increases cerebrovascular ROS levels. Using confocal microscopy, acute Ang II infusion intravenously increased ROS production by cerebral blood vessels in the cerebral cortex that was dependent on activation of AT1 receptors and Nox2 NADPH oxidase (Girouard et al., 2006, 2007). Thus, acute in vitro or in vivo Ang II treatment increases cerebrovascular NADPH oxidase-derived ROS. The majority of evidence to date suggests Nox2 NADPH oxidase is the primary source of Ang II-stimulated ROS in cerebral arteries. However, there is some evidence to suggest that Ang II may also activate Nox1 NADPH oxidase in cerebral blood vessels.

In addition to acute stimulation of ROS production, Ang II elevates cerebrovascular ROS levels when administered more chronically. For example, cerebrovascular O2·− production is elevated following 7-days Ang II treatment. Specifically, both basal and Nox2 stimulated O2·− production by cerebral arteries was elevated following Ang II treatment (Chrissobolis et al., 2012). Consistent with previous studies that have reported a key role for Nox2 NADPH oxidase in Ang II-stimulated ROS production, ROS production by cerebral arteries from Nox2 deficient mice treated with Ang II was not elevated (Chrissobolis et al., 2012). In support of these findings, studies using genetically altered mice with life-long Ang II-dependent hypertension also suggest that increased O2·− underlies impaired endothelial function in cerebral arteries (Faraci et al., 2006).

In another model of chronic Ang II-dependent hypertension, using a lower dose of Ang II to produce a delayed increase in blood pressure (also known as a “slow-pressor” dose of Ang II), ROS levels in the vasculature are elevated (Capone et al., 2010, 2011, 2012). Specifically, using dihydroethidium fluorescence (to detect ROS) and immunofluorescence (to stain for cell-specific markers), increases in ROS levels were localized to the endothelium as well as neurons. This elevation in ROS was associated with impaired cerebrovascular responses to endothelium-dependent agonists (Capone et al., 2011, 2012). Scavenging O2·− reduced ROS levels and ameliorated the impaired vasodilation, indicating that the increases in O2·− were functionally important (Capone et al., 2011). Using this same model, Ang II infusion produced an increase in oxidative stress within the subfornical organ (SFO) (Capone et al., 2012), a specialized circumventricular organ which can sense changes in plasma Ang II levels and participates in central mechanisms that promote hypertension. Increased oxidative stress in the SFO paralleled increases in circulating arginine vasopressin (AVP) and increased perivascular concentrations of endothelin-1 (ET-1) (Capone et al., 2012). Ultimately, both ET-1 and Ang II mediated the elevation in ROS in the vasculature (Capone et al., 2012), presumably via activation of Nox2 NADPH oxidase. Overall, this recent study suggested that the SFO, though effects on AVP and ET-1, contributes to Ang II-induced oxidative stress.

Alterations to cerebrovascular function

In addition to being a strong stimulus of NADPH oxidase activity, Ang II is a potent vasoconstrictor. Studies of cerebral arteries, including those in humans, have found that Ang II produces vasoconstriction (Whalley et al., 1985, 1987; Toda et al., 1990; Bevan et al., 1998; Stenman and Edvinsson, 2004; Miller et al., 2005; Vincent et al., 2005; Faraci et al., 2006; De Silva et al., 2009; Amberg et al., 2010; Ahnstedt et al., 2011). Both genetic and pharmacological approaches have established that Ang II induces constriction of cerebral arteries via activation of AT1 receptors (Naveri et al., 1994; Stenman and Edvinsson, 2004; Faraci et al., 2006). Classical Ang II-induced contraction of smooth muscle occurs via AT1 receptor mediated activation of phospholipase C, resulting in inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) production. IP3 and DAG regulate two distinct pathways, however, both ultimately result in smooth muscle contraction via activation of a number of protein kinases, such as myosin light chain kinase and Rho-kinase (Hilgers and Webb, 2005). Indeed, Ang II-induced constriction of basilar arteries is abolished in the presence of the Rho-kinase inhibitor, Y-27632 (Faraci et al., 2006).

While some effects of Ang II can clearly be mediated by AT1 receptors on vascular muscle, double-label immunoelectron microscopy and other approaches have demonstrated that AT1 receptors are also present in cerebral endothelium where Ang II can produce endothelium-dependent constriction via cyclooxygenase- and/or O2·−-dependent mechanisms (Manabe et al., 1989; Kazama et al., 2004; De Silva et al., 2009). As noted above, ROS are important modulators of vascular tone and Ang II-induced constriction of cerebral arteries in mice are mediated by H2O2 (De Silva et al., 2009). Considering this and the aforementioned findings together, it is possible that Ang II-induced constriction of cerebral arteries occurs via stimulation of ROS production, which in turn leads to the activation of Rho-kinase. While Rho-kinase is not generally thought to be a redox sensitive enzyme, the activity of the small G-protein RhoA, the principal activator of Rho-kinase, is redox-sensitive and thus may be modulated by ROS. Indeed, both O2·− and t-butyl hydroperoxide (a stable analog of H2O2) can activate RhoA (Jin et al., 2004; Jernigan et al., 2008; Aghajanian et al., 2009; Broughton et al., 2009). Specifically, oxidation of two cysteine residues in the phosphoryl binding loop of RhoA by t-butyl hydroperoxide results in the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP), and activation of RhoA (Aghajanian et al., 2009). Once active, RhoA then translocates to the cell membrane where it binds to and activates Rho-kinase (Leung et al., 1995). This may result in signaling via the well-established pathway of Rho-kinase-dependent inhibition of myosin light chain phosphatase (MLCP), thus preventing the de-phosphorylation of myosin (Kimura et al., 1996; Jin et al., 2004; Jernigan et al., 2008). While this may be a potential signaling pathway by which Ang II induced constriction in cerebral arteries, further studies are needed to clarify the role of ROS in vascular responses to Ang II.

In addition to direct effects on vasomotor tone, Ang II impairs endothelium-dependent vasodilation by reducing the bioavailability of NO· and potentially by interfering with other endothelium-dependent mechanisms. This effect is intimately linked with the increased production of ROS (mainly O2·−) in response to Ang II and resulting impairment of NO·-mediated signaling (Figure 2). Consistent with this concept, numerous studies indicate that Ang II has deleterious effects on cerebrovascular function. Indeed, in multiple models (both acute and chronic administration) (Gerzanich et al., 2003; Faraci et al., 2006; Kitayama et al., 2006; Chrissobolis and Faraci, 2010; Chrissobolis et al., 2012), Ang II impairs endothelium-dependent vasodilation cerebral blood vessels. Treatment with scavengers of O2·− improved endothelial function, thus indicating that oxidative stress played a key role in producing this dysfunction (Faraci et al., 2006; Kitayama et al., 2006; Chrissobolis and Faraci, 2010). A recent study has provided, further evidence supporting a role for NADPH oxidase-derived ROS in causing oxidative stress in response to Ang II treatment. Specifically, 7-days Ang II treatment did not result in impaired endothelium-dependent vasodilatation in arteries from Nox2 deficient mice (Chrissobolis et al., 2012). This same study reported a small role for the Nox1-containing NADPH oxidase in this model of Ang II-induced dysfunction (Chrissobolis et al., 2012). Further studies will be needed to better define the role of this isoform of NADPH oxidase as well as Nox4- and Nox5-containing NADPH oxidase in hypertension as well as other disease states.

Figure 2.

Figure 2

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Angiotensin II (Ang II) promotes oxidative stress in the vasculature via stimulation of AT1 receptors and subsequent activation of NADPH oxidases. Oxidative stress contributes to blood-brain barrier (BBB) dysfunction, impairment of vasodilation and neurovascular coupling, and promotes inward vascular remodeling and inflammation. Overall, these effects contribute to reductions in cerebral blood flow, which can lead to dementia and increased susceptibility for stroke and lessen recovery following stroke or other forms of brain injury.

Effects on neurovascular coupling

Many cell types including vascular muscle and endothelial cells, neurons and glia work together to supply oxygen and glucose to support the dynamic energy demands of the brain. This interaction between the cells within the parenchyma has given rise to the concept of a “neurovascular unit,” a multi-cellular structure with unique features and functions. For example, cells within the neurovascular unit are part of one segment of the circulation that contributes to regulation of cerebral blood flow during changes in metabolic demands that result from increased brain activity. This relationship between cellular activity and local cerebral blood flow is often termed neurovascular coupling or functional hyperemia. Cellular activity increases the need of brain cells for oxygen and nutrients, as well as the removal of metabolic by-products by the circulating blood. Specifically, when neurons and/or glia are active and require more energy substrates, the release of paracrine factors and other mechanisms produce dilation of local cerebral arterioles and upstream vessels supplying the region resulting in an increase in local cerebral blood flow. Several factors have been suggested to mediate the neurovascular coupling response. NO· (Iadecola et al., 1995; Liu et al., 2008), K+ ions (Filosa et al., 2006), arachidonic acid metabolites (Niwa et al., 2000; Liu et al., 2008) as well as products of cellular metabolism such as adenosine (Dirnagl et al., 1994; Meno et al., 2001) have all been proposed to contribute to neurovascular coupling. These factors are release by neurons/glial cells and activate receptors/ion channels on cerebral blood vessels leading to vasodilation and a subsequent increase in local cerebral blood flow. It has also been suggested that the identity of the factor(s) mediating the neurovascular coupling response is dependent on the brain region and multiple agents may act in concert to produce the full vasodilatory response (Iadecola and Nedergaard, 2007).

Neurovascular coupling is affected by both Ang II and oxidative stress (Figure 2). Both acute and chronic systemic administration of Ang II increases arterial blood pressure. Irrespective of the length of treatment, Ang II-induced hypertension was associated with impairment of the neurovascular coupling response to whisker stimulation (Kazama et al., 2003, 2004; Girouard et al., 2007, 2008; Capone et al., 2009, 2010, 2011). Interestingly, in mice that were administered phenylephrine acutely to produce a similar degree of hypertension, there was no impairment of neurovascular coupling (Kazama et al., 2003; Capone et al., 2011), suggesting that Ang II and not the elevation in systemic pressure per se caused the neurovascular dysfunction. This impaired neurovascular coupling could be restored to normal by treatment with O2·− scavengers (Kazama et al., 2004; Capone et al., 2011), suggesting a key role for oxidative stress. Further studies indicated that Nox2 NADPH oxidase was the likely source of increased ROS as Nox2-deficient mice were protected from Ang II-dependent neurovascular dysfunction (Kazama et al., 2004; Girouard et al., 2007). Ang II has also been shown to increase 3-nitrotyrosine immunoreactivity (a marker for ONOO− formation), in a Nox2 NADPH oxidase-dependent manner in cerebral vessels (Girouard et al., 2007), suggesting that Ang II-dependent hypertension causes oxidative stress and vascular dysfunction within components of the neurovascular unit. Furthermore, it was also shown that a ONOO− scavenger or a ONOO− decomposition catalyst protect against Ang II dysfunction (Girouard et al., 2007). These findings suggest that ONOO− may be a key intermediate of neurovascular dysfunction in response to Ang II.

The presence of the blood-brain barrier (BBB) is a unique characteristic of cerebral blood vessels with many functions including regulation of influx and efflux of cells and molecules between the blood and the brain. There is emerging evidence that Ang II and oxidative stress have detrimental effects on BBB permeability (Figure 2). For example, Ang II increases the permeability of cerebral microvascular endothelial cells in culture (Guillot and Audus, 1991; Fleegal-Demotta et al., 2009), which may contribute to increases in BBB permeability in vivo. Indeed, studies of Ang II-dependent hypertensive mice have found that BBB permeability is increased compared with vehicle treated mice (Vital et al., 2010; Zhang et al., 2010). Feeding of a high salt diet to Ang II treated mice did not augment BBB permeability, suggesting that Ang II and not increases in arterial pressure per se was the mediator of BBB dysfunction (Vital et al., 2010). Along these lines, DOCA (deoxycorticosterone acetate)/salt-induced hypertension is not associated with increased blood brain barrier permeability (Rodrigues and Granger, 2011). Oxidative stress is likely to be a key mediator of BBB dysfunction as treatment of Ang II-dependent hypertensive mice with a O2·− scavenger prevents BBB dysfunction (Zhang et al., 2010). The source of ROS and the mechanisms by which BBB permeability is increased during Ang II-dependent hypertension remains unclear. However, considering that Nox1 and Nox2 NADPH oxidase are important sources of Ang II-stimulated ROS in the cerebral circulation, these enzymes may play a key role.

Alterations to vascular structure

One of the mechanisms that can contribute to vascular dysfunction and impact cerebral blood flow is alterations to the structure of cerebral arteries and arterioles. Hypertension is associated with hypertrophy of large conduit vessels, such as the aorta, whereas it causes inward remodeling of smaller resistance vessels, including cerebral arterioles (Figure 2; Baumbach and Heistad, 1989; Baumbach et al., 2003). This latter structural change results in a smaller diameter lumen even when the vessel is maximally dilated (Baumbach and Heistad, 1989; Baumbach et al., 2003) and has emerged as a potential risk factor for cardiovascular and cerebrovascular events (Faraci, 2011). Ang II appears to be a critical mediator of inward remodeling as cerebral arterioles undergo remodeling and hypertrophy in Ang II-dependent models of hypertension, whereas Ang II-independent hypertension only causes hypertrophy (Baumbach et al., 2003). Furthermore, preliminary studies suggest Nox2 NADPH oxidase may be the critical link between Ang II and inward remodeling as cerebral arterioles from Nox2 deficient mice do not undergo inward remodeling following Ang II infusion (Chan and Baumbach, 2009). Inward remodeling impairs vasodilator capacity and thus can compromise brain perfusion and may contribute to impaired cognitive function in people with hypertension.

Cerebrovascular inflammation

Hypertension induces an inflammatory phenotype within the cerebral vasculature and Ang II is believed to be a key mediator of this effect (Figure 2). For example, spontaneously hypertensive rats (SHR) have elevated expression of the pro-inflammatory marker intercellular adhesion molecule 1 (ICAM-1) (Ando et al., 2004). Interestingly, treatment of SHR with an AT1 receptor antagonist reduced the expression of ICAM-1, indicating a key role for Ang II (Ando et al., 2004). The increased expression of this adhesion molecule was associated with increased adhesion and infiltration of macrophages into the brain of SHR (Ando et al., 2004). As with ICAM-1 expression, treatment with an AT1 receptor antagonist prevented the increased macrophage infiltration (Ando et al., 2004). More recently, it has been demonstrated that there is an increase in leukocyte and platelet adhesion to the microvasculature in Ang II-induced hypertension (Vital et al., 2010; Zhang et al., 2010). The increased leukocyte/platelet adhesion observed during Ang II-induced hypertension is dependent on AT1 receptors on leukocytes/platelets (Vital et al., 2010) and ROS (Zhang et al., 2010), indicating that oxidative stress may play a key role. Similarly, in a DOCA/salt model of hypertension, leukocyte and platelet adhesion is dependent on activation of AT1 receptors and mitochondrial-derived ROS (Rodrigues and Granger, 2011). The source of the ROS contributing to augmented leukocyte and platelet adhesion to the cerebral microvasculature warrants further investigation. However, due to the link between AT1 receptors and Nox2 NADPH oxidase, it is possible that this isoform of NADPH oxidase plays a key role.

Sex-dependent differences in cerebrovascular responses to Ang II

It is well-known that the incidence of cerebrovascular disease is lower in pre-menopausal females compared with males and post-menopausal females (Prencipe et al., 1997; Appelros et al., 2009). At present study, the precise reasons for the sex-dependent differences in disease incidence remain to be fully defined. However, the fact that Ang II has sexually dimorphic effects on the cerebral vasculature may contribute to the observed difference in disease incidence. Acute Ang II treatment increases O2·− and H2O2 levels to a greater extent in arteries from males compared with females (De Silva et al., 2009). Indeed, sex differences in Ang II stimulated ROS was completely dependent on Nox2 NADPH oxidase (De Silva et al., 2009). This effect is consistent with other previous reports that have shown that Ang II exerts sexually dimorphic effects with regards to vasoconstriction (Faraci et al., 2006) and susceptibility to vascular dysfunction (Girouard et al., 2008; Chrissobolis and Faraci, 2010). This effect of sex on cerebrovascular ROS levels, vasoconstriction and neurovascular dysfunction in response to Ang II is likely due to the protective effects of estrogen on the cerebral circulation. Specifically, cerebrovascular ROS levels in response to Ang II are only elevated during diestrus (low estrogen levels) and Ang II does not significantly elevate ROS levels during proestrus and estrus (Capone et al., 2009). Due to the increase in oxidative stress, female mice in diestrus were susceptible to Ang II-induced neurovascular dysfunction (Capone et al., 2009). Currently, the mechanisms by which estrogen suppresses Ang II-induced oxidative stress in the cerebral circulation remains to be determined. If therapies are developed that can exploit this phenomenon, it may prove to be an effective therapy to reduce cerebrovascular oxidative stress.

Effects on cerebral blood flow in humans

Hypertension has detrimental effects on cerebral blood flow in humans (Lipsitz et al., 2005; Beason-Held et al., 2007; Dai et al., 2008; Efimova et al., 2008; Nagata et al., 2010; Waldstein et al., 2010; Muller et al., 2012). Hypertension is associated with a shift in the autoregulatory curve to the right (Cipolla, 2007; Iadecola and Davisson, 2008). This means that during hypertension, higher pressures are needed to maintain cerebral perfusion in the normal range. Therefore, an aggressive blood pressure lowering regime may result in cerebral hypoperfusion and adverse outcomes. Despite the aforementioned concerns, a number of experimental studies have reported that anti-hypertensive therapies that target the RAS improve cerebral blood flow. As discussed above, Ang II has a number of diverse effects on the cerebral circulation, including effects on vascular tone, which may lead to reductions in cerebral blood flow (Figure 2). As such, it is not surprising that treatment with either ACE inhibitors (Lipsitz et al., 2005; Efimova et al., 2008) or AT1 receptor antagonists (Oku et al., 2005; Kimura et al., 2010; Nagata et al., 2010; Muller et al., 2012) improves or maintains total cerebral blood flow in hypertensive patients. Recently however, the Second Manifestations of ARTerial disease-Magnetic Resonance (SMART-MR) study reported that despite effective treatment (reduction of BP to <140/90 mmHg) neither ACE inhibitors or other non RAS based therapies (β-blocker, diuretic or calcium channel blocker) prevented the decline in cerebral blood flow that occurs in elderly hypertensive individuals over an average 3.9 years follow up period (Muller et al., 2012). In contrast, effective treatment (BP <140/90 mmHg) of hypertensive patients with an AT1 receptor antagonists prevented the decline in cerebral blood flow (Muller et al., 2012). The inability of ACE inhibitors to improve cerebral blood flow may suggest that AT1 receptor antagonism provides additional benefits over reducing circulating Ang II levels. One possibility is that inhibition of AT1 receptors allows circulating Ang II to signal via AT2 receptors. AT2 receptor activation has a number of potential beneficial effects on the vasculature, many of which oppose with effects of AT1 receptor activation (e.g., vasodilatation). As oxidative stress is a central component of Ang II-induced cerebrovascular dysfunction it would be expected that oxidative stress contributes to changes in cerebral blood flow during hypertension. However, further studies are needed to clarify whether oxidative stress plays a role in alterations to cerebral perfusion associated with hypertension in humans and which class of therapeutics would be of most benefit in reducing hypertension, while maintaining cerebral perfusion.

Increases in blood flow produce flow-mediated (or flow-dependent) dilation of cerebral arteries in rats in vivo (Fujii et al., 1991, 1992; Paravicini et al., 2006). A similar dilator mechanism appears to be present in humans and may be impacted by hypertension. For example, hypertension impairs increases in cerebral blood flow caused by mental stress (Naqvi and Hyuhn, 2009). Dilation of the common carotid artery as well as increases in blood flow velocity in the middle cerebral artery in response to mental stress are selectively impaired in hypertensive patients compared with normotensive controls (Naqvi and Hyuhn, 2009). These findings in humans are consistent with studies in animal models which suggest that hypertension impairs neurovascular coupling and mechanisms that provide critical support for functional hyperemia responses (flow-mediated dilation of large upstream vessels with the resulting maintenance of local microvascular pressure). If Ang II and oxidative stress similarly contribute to the impaired neurovascular coupling, therapeutically targeting the RAS may be of benefit.

Conclusion

Ang II has prominent effects on the cerebral circulation. Many of these effects are deleterious and occur as a result of oxidative stress and the accompanying vascular dysfunction. Overall, the dysfunction caused by Ang II may result in compromised cerebral perfusion and may ultimately predispose the brain the hypoperfusion-dependent cognitive abnormalities as well as ischemic events. The vast majority of experimental studies to date implicate Nox2 NADPH oxidase-derived ROS as the major source of O2·− and thus a key mediator of Ang II-induced oxidative stress. Due to the sensitivity of cerebral arteries to ROS, alterations to cerebrovascular function in response to Ang II may occur prior to changes in other parts of the vasculature. As such, additional studies that further define the role of Ang II and oxidative stress in the pathogenesis of cerebrovascular diseases will prove crucial. Furthermore, identification of molecules that protect the cerebral circulation and the brain against the effects of Ang II and oxidative stress may be of substantial benefit.

Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Acknowledgments

Work by the authors is supported by National Institutes of Health grants HL-38901, NS-24621, HL-62984, and HL-113863 as well as funds from the Department of Veterans Affairs (BX001399). T. Michael De Silva is the recipient of an American Heart Association Postdoctoral Fellowship (12POST9150027).

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