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. 1978 Apr;26(1):61–70. doi: 10.1128/jvi.26.1.61-70.1978

Synthesis of defective viral DNA in HeLa cells infected with adenovirus type 3.

E Daniell, T Mullenbach
PMCID: PMC354034  PMID: 650739

Abstract

Virus-specific DNA fragments that are shorter than the full-length viral genomes have been isolated from HeLa cells productively infected with adenovirus type 3. A number of predominant size classes could be detected by gel electrophoresis and hybridization, and the array of sizes was similar or identical to the selection in DNA purified from incomplete particles of this serotype (E. Daniell, J. Virol. 19:685-708, 1976). A large fraction of these short DNA molecules contained long inverted terminal repetitions, as did DNA molecules from incomplete particles. Restriction analysis showed that these subgenomic molecules consist of sequences from the two molecular ends of the normal genome. These results suggest that the predominance of left-hand end fragments seen in packaged incomplete DNAs results from selective packaging, whereas the predominance of certain size classes of intracellular viral DNA is a function of prepackaging events. The incomplete DNAs were generated at all times during viral DNA replication, and the yield relative to complete DNA did not seem to vary significantly with time or multiplicity of infection or when the virus was propagated on different human cell types.

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Selected References

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  1. Edvardsson B., Everitt E., Jörnvall H., Prage L., Philipson L. Intermediates in adenovirus assembly. J Virol. 1976 Aug;19(2):533–547. doi: 10.1128/jvi.19.2.533-547.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Flint S. J., Berget S. M., Sharp P. A. Characterization of single-stranded viral DNA sequences present during replication of adenovirus types 2 and 5. Cell. 1976 Dec;9(4 Pt 1):559–571. doi: 10.1016/0092-8674(76)90038-6. [DOI] [PubMed] [Google Scholar]
  3. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  5. Holland J. J., Villarreal L. P., Breindl M. Factors involved in the generation and replication of rhabdovirus defective T particles. J Virol. 1976 Mar;17(3):805–815. doi: 10.1128/jvi.17.3.805-815.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Honda B. M., Baillie D. L., Candido E. P. Properties of chromatin subunits from developing trout testis. J Biol Chem. 1975 Jun 25;250(12):4643–4647. [PubMed] [Google Scholar]
  7. Horwitz M. S. Bidirectional replication of adenovirus type 2 DNA. J Virol. 1976 Apr;18(1):307–315. doi: 10.1128/jvi.18.1.307-315.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Horwitz M. S., Brayton C., Baum S. G. Synthesis of type 2 adenovirus DNA in the presence of cycloheximide. J Virol. 1973 Apr;11(4):544–551. doi: 10.1128/jvi.11.4.544-551.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Horwitz M. S. Location of the origin of DNA replication in adenovirus Type 2. J Virol. 1974 May;13(5):1046–1054. doi: 10.1128/jvi.13.5.1046-1054.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ishibashi M., Maizel J. V., Jr The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology. 1974 Feb;57(2):409–424. doi: 10.1016/0042-6822(74)90181-0. [DOI] [PubMed] [Google Scholar]
  11. Ketner G., Kelly T. J., Jr Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases. Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102–1106. doi: 10.1073/pnas.73.4.1102. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Lavelle G., Patch C., Khoury G., Rose J. Isolation and partial characterization of single-stranded adenoviral DNA produced during synthesis of adenovirus type 2 DNA. J Virol. 1975 Oct;16(4):775–782. doi: 10.1128/jvi.16.4.775-782.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mak S. Defective virions in human adenovirus type 12. J Virol. 1971 Apr;7(4):426–433. doi: 10.1128/jvi.7.4.426-433.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Maniatis T., Jeffrey A., Kleid D. G. Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184–1188. doi: 10.1073/pnas.72.3.1184. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pettersson U. Letter: Some unusual properties of replicating adenovirus type 2 DNA. J Mol Biol. 1973 Dec 25;81(4):521–527. doi: 10.1016/0022-2836(73)90521-4. [DOI] [PubMed] [Google Scholar]
  16. Prage L., Höglund S., Philipson L. Structural proteins of adenoviruses. 8. Characterization of incomplete particles of adenovirus type 3. Virology. 1972 Sep;49(3):745–757. doi: 10.1016/0042-6822(72)90531-4. [DOI] [PubMed] [Google Scholar]
  17. Schilling R., Weingärtner B., Winnacker E. L. Adenovirus type 2 DNA replication. II. Termini of DNA replication. J Virol. 1975 Oct;16(4):767–774. doi: 10.1128/jvi.16.4.767-774.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tjia S., Fanning E., Schick J., Doerfler W. Incomplete particles of adenovirus type 2. III. Viral and cellular DNA sequences in incomplete particles. Virology. 1977 Jan;76(1):365–379. doi: 10.1016/0042-6822(77)90309-9. [DOI] [PubMed] [Google Scholar]
  19. Tolun A., Pettersson U. Termination sites for adenovirus type 2 DNA replication. J Virol. 1975 Oct;16(4):759–766. doi: 10.1128/jvi.16.4.759-766.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Weingärtner B., Winnacker E. L., Tolun A., Pettersson U. Two complementary strand-specific termination sites for adenovirus DNA replication. Cell. 1976 Oct;9(2):259–268. doi: 10.1016/0092-8674(76)90117-3. [DOI] [PubMed] [Google Scholar]

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