Abstract
Moloney murine leukemia virus was harvested automatically within 60 min of release from chronically infected NIH/3T3 cells (clone 1) cultured on bundles of synthetic capillaries. Production of virus as measured by a determination of reverse transcriptase activity and by the XC syncytia assay demonstrated that highly infectious "rapid-harvest" virus was recovered from NIH/3T3 cells (clone 1) grown for periods of up to 10 days.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cheung K. S., Smith R. E., Stone M. P., Joklik W. K. Comparison of immature (rapid harvest) and mature Rous sarcoma virus particles. Virology. 1972 Dec;50(3):851–864. doi: 10.1016/0042-6822(72)90439-4. [DOI] [PubMed] [Google Scholar]
- Chick W. L., Like A. A., Lauris V. Beta cell culture on synthetic capillaries: an artificial endocrine pancreas. Science. 1975 Mar 7;187(4179):847–849. doi: 10.1126/science.187.4179.847. [DOI] [PubMed] [Google Scholar]
- Duesberg P., Canaani E., Von der Helm K. Evidence for 30-40S RNA as precursor of the 60-70S RNA of Rous sarcoma virus. Am J Clin Pathol. 1973 Jul;60(1):57–64. doi: 10.1093/ajcp/60.1.57. [DOI] [PubMed] [Google Scholar]
- Flügel R. M., Rapp U., Wells R. D. RNA-DNA covalent bonds between the RNA primers and the DNA products formed by RNA tumor virus DNA polymerase. J Virol. 1973 Dec;12(6):1491–1502. doi: 10.1128/jvi.12.6.1491-1502.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Manly K. F. A continuous-flow system for the growth of RNA tumor viruses. Anal Biochem. 1975 Feb;63(2):491–500. doi: 10.1016/0003-2697(75)90373-5. [DOI] [PubMed] [Google Scholar]
- McClain K., Kirsten W. H. Electrophoretic analysis of the RNA from a mouse leukemia virus. Cancer Res. 1972 Jul;32(7):1470–1475. [PubMed] [Google Scholar]
- Paskind M. P., Weinberg R. A., Baltimore D. Dependence of Moloney murine leukemia virus production on cell growth. Virology. 1975 Sep;67(1):242–248. doi: 10.1016/0042-6822(75)90421-3. [DOI] [PubMed] [Google Scholar]
- Rapp U. R., Nowinski R. C. Endogenous ecotropic mouse type C viruses deficient in replication and production of XC plaques. J Virol. 1976 May;18(2):411–417. doi: 10.1128/jvi.18.2.411-417.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rothenberg E., Baltimore D. Synthesis of long, representative DNA copies of the murine RNA tumor virus genome. J Virol. 1975 Jan;17(1):168–174. doi: 10.1128/jvi.17.1.168-174.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rowe W. P., Pugh W. E., Hartley J. W. Plaque assay techniques for murine leukemia viruses. Virology. 1970 Dec;42(4):1136–1139. doi: 10.1016/0042-6822(70)90362-4. [DOI] [PubMed] [Google Scholar]
- Smith R. E., Kozoman F. Device for automatic rapid harvest of roller culture supernatant fluid. Appl Microbiol. 1973 Jun;25(6):1008–1010. doi: 10.1128/am.25.6.1008-1010.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stull H. B., Gazdar A. F. Stability of Rauscher leukemia virus under certain laboratory conditions. Proc Soc Exp Biol Med. 1976 Sep;152(4):554–556. doi: 10.3181/00379727-152-39438. [DOI] [PubMed] [Google Scholar]
- Temin H. M., Baltimore D. RNA-directed DNA synthesis and RNA tumor viruses. Adv Virus Res. 1972;17:129–186. doi: 10.1016/s0065-3527(08)60749-6. [DOI] [PubMed] [Google Scholar]