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. 1978 Jul;27(1):182–192. doi: 10.1128/jvi.27.1.182-192.1978

Isolation and preliminary characterization of temperature-sensitive mutants of encephalomyocarditis virus.

R J Radloff
PMCID: PMC354151  PMID: 211249

Abstract

Thirty temperature-sensitive mutants of encephalomyocarditis virus have been isolated and partially characterized. Fifteen of these mutants are phenotypically RNA+ thirteen are RNA-, and two are RNA +/-. Six RNA + mutants, one RNA- mutants, and one RNA +/- mutant have virions which are more thermosensitive at 56 degree C than the wild-type virions. Hela cells infected at the nonpermissive temperature with any of the RNA+ mutants produced neither infective nor noninfective viral particles. The cleavage of the precursor polypeptides in cells infected with 11 of the RNA+ mutants was defective at the nonpermissive temperature. This defect in cleavage occurred only in those precursor polypeptides leading to capsid proteins.

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Selected References

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