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. 1978 Jul;27(1):255–257. doi: 10.1128/jvi.27.1.255-257.1978

Dextran sulfates as a contaminant of DNA extracted from concentrated viruses and as an inhibitor of DNA polymerases.

R A Hitzeman, A M Hanel, A R Price
PMCID: PMC354157  PMID: 357755

Abstract

Dextran sulfate is commonly used with polyethylene glycol to concentrate viruses before extraction of their DNA. However, dextran slulfate then easily contaminated such DNA and acted as a potent inhibitor of DNA polymerases from Bacillus subtilis (III), phage PBS2, and phage T4. Dextran sulfate only weakly inhibited Micrococcus luteus and Escherichia coli DNA polymerase I preparations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENGTSSON S. MECHANISM OF DEXTRAN SULFATE INHIBITION OF ATTENUATED POLIOVIRUS. Proc Soc Exp Biol Med. 1965 Jan;118:47–53. doi: 10.3181/00379727-118-29752. [DOI] [PubMed] [Google Scholar]
  2. Bachrach U., Friedmann A. Practical procedures for the purification of bacterial viruses. Appl Microbiol. 1971 Oct;22(4):706–715. doi: 10.1128/am.22.4.706-715.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clements J. E., D'Ambrosio J., Brown N. C. Inhibition of Bacillus subtilis deoxyribonucleic acid polymerase III by phenylhydrazinopyrimidines. Demonstration of a drug-induced deoxyribonucleic acid-enzyme complex. J Biol Chem. 1975 Jan 25;250(2):522–526. [PubMed] [Google Scholar]
  4. Gass K. B., Cozzarelli N. R. Bacillus subtilis DNA polymerases. Methods Enzymol. 1974;29:27–38. doi: 10.1016/0076-6879(74)29006-2. [DOI] [PubMed] [Google Scholar]
  5. Gass K. B., Low R. L., Cozzarelli N. R. Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines. Proc Natl Acad Sci U S A. 1973 Jan;70(1):103–107. doi: 10.1073/pnas.70.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jovin T. M., Englund P. T., Bertsch L. L. Enzymatic synthesis of deoxyribonucleic acid. XXVI. Physical and chemical studies of a homogeneous deoxyribonucleic acid polymerase. J Biol Chem. 1969 Jun 10;244(11):2996–3008. [PubMed] [Google Scholar]
  7. Marcus M., Newlon M. C. Control of DNA synthesis in Bacillus subtilis by phage phi e. Virology. 1971 Apr;44(1):83–93. doi: 10.1016/0042-6822(71)90155-3. [DOI] [PubMed] [Google Scholar]
  8. Miyazawa F., Olijnyk O. R., Tilley C. J., Tamaoki T. Interactions between dextran sulfate and Escherichia coli ribosomes. Biochim Biophys Acta. 1967 Aug 22;145(1):96–104. doi: 10.1016/0005-2787(67)90658-2. [DOI] [PubMed] [Google Scholar]
  9. Price A. R., Cook S. J. New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2. J Virol. 1972 Apr;9(4):602–610. doi: 10.1128/jvi.9.4.602-610.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vazquez D., Monro R. E. Effects of some inhibitors of protein synthesis on the binding of aminoacyl tRNA to ribosomal subunits. Biochim Biophys Acta. 1967 Jun 20;142(1):155–173. doi: 10.1016/0005-2787(67)90524-2. [DOI] [PubMed] [Google Scholar]
  11. Wu R. Inhibition of polynucleotide kinase by agar, dextran sulfate and other polysaccharide sulfates. Biochem Biophys Res Commun. 1971 May 21;43(4):927–934. doi: 10.1016/0006-291x(71)90706-6. [DOI] [PubMed] [Google Scholar]
  12. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large-scale virus purification. Virology. 1970 Mar;40(3):734–744. doi: 10.1016/0042-6822(70)90218-7. [DOI] [PubMed] [Google Scholar]

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