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. 1978 Aug;27(2):427–431. doi: 10.1128/jvi.27.2.427-431.1978

Polyoma virus has three late mRNA's: one for each virion protein.

S G Siddell, A E Smith
PMCID: PMC354180  PMID: 211268

Abstract

Polyoma virus mRNA, isolated from the cytoplasm of 3T6 cells late after infection and purified by hybridization to HpaII fragment 3 of polyoma virus DNA, was separated on 50% formamide-containing sucrose density gradients, and the fractionated RNA was recovered and translated in vitro. Analysis of the cell-free products showed that the minor virion protein VP3 was synthesized from an mRNA sedimenting at approximately 18S betweeen the 19S VP2 mRN and the 16S VP1 mRNA. Other experiments showed that the VP2 and VP3 can be labeled with formyl methionine from initiator tRNA. We conclude that there are three late polyoma virus mRNA's, each directing the synthesis of only one viral capsid protein.

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Selected References

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  1. Aloni Y., Dhar R., Laub O., Horowitz M., Khoury G. Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3686–3690. doi: 10.1073/pnas.74.9.3686. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Anderson C. W., Lewis J. B., Atkins J. F., Gesteland R. F. Cell-free synthesis of adenovirus 2 proteins programmed by fractionated messenger RNA: a comparison of polypeptide products and messenger RNA lengths. Proc Natl Acad Sci U S A. 1974 Jul;71(7):2756–2760. doi: 10.1073/pnas.71.7.2756. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  4. Birg F., Favaloro J., Kamen R. Analysis of polyoma virus nuclear RNA by mini-blot hybridization. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3138–3142. doi: 10.1073/pnas.74.8.3138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Buetti E. Characterization of late polyoma mRNA. J Virol. 1974 Aug;14(2):249–260. doi: 10.1128/jvi.14.2.249-260.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cole C. N., Landers T., Goff S. P., Manteuil-Brutlag S., Berg P. Physical and genetic characterization of deletion mutants of simian virus 40 constructed in vitro. J Virol. 1977 Oct;24(1):277–294. doi: 10.1128/jvi.24.1.277-294.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Contreras R., Rogiers R., Van de Voorde A., Fiers W. Overlapping of the VP2-VP3 gene and the VP1 gene in the SV40 genome. Cell. 1977 Oct;12(2):529–538. doi: 10.1016/0092-8674(77)90129-5. [DOI] [PubMed] [Google Scholar]
  8. De Robertis E. M., Mertz J. E. Coupled transcription-translation of DNA injected into Xenopus oocytes. Cell. 1977 Sep;12(1):175–182. doi: 10.1016/0092-8674(77)90195-7. [DOI] [PubMed] [Google Scholar]
  9. Fey G., Hirt B. Fingerprints of polyoma virus proteins and mouse histones. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):235–241. doi: 10.1101/sqb.1974.039.01.030. [DOI] [PubMed] [Google Scholar]
  10. Fiers W., Contreras R., Haegemann G., Rogiers R., Van de Voorde A., Van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M. Complete nucleotide sequence of SV40 DNA. Nature. 1978 May 11;273(5658):113–120. doi: 10.1038/273113a0. [DOI] [PubMed] [Google Scholar]
  11. Gibson W., Hunter T., Cogen B., Eckhart W. Altered virion proteins of a temperature-sensitive mutant of polyoma virus, ts59. Virology. 1977 Jul 1;80(1):21–41. doi: 10.1016/0042-6822(77)90378-6. [DOI] [PubMed] [Google Scholar]
  12. Gibson W. Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology. 1974 Dec;62(2):319–336. doi: 10.1016/0042-6822(74)90395-x. [DOI] [PubMed] [Google Scholar]
  13. Glanville N., Ranki M., Morser J., Käriäinen L., Smith A. E. Initiation of translation directed by 42S and 26S RNAs from Semliki Forest virus in vitro. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3059–3063. doi: 10.1073/pnas.73.9.3059. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Griffin B. E. Fine structure of polyoma virus DNA. J Mol Biol. 1977 Dec 5;117(2):447–471. doi: 10.1016/0022-2836(77)90137-1. [DOI] [PubMed] [Google Scholar]
  15. Hewick R. M., Fried M., Waterfield M. D. Nonhistone virion proteins of polyoma: characterisation of the particle proteins by tryptic peptide analysis by use of ion-exchange columns. Virology. 1975 Aug;66(2):408–419. doi: 10.1016/0042-6822(75)90213-5. [DOI] [PubMed] [Google Scholar]
  16. Hsu M. T., Ford J. Sequence arrangement of the 5' ends of simian virus 40 16S and 19S mRNAs. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4982–4985. doi: 10.1073/pnas.74.11.4982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Kamen R., Shure H. Topography of polyoma virus messenger RNA molecules. Cell. 1976 Mar;7(3):361–371. doi: 10.1016/0092-8674(76)90165-3. [DOI] [PubMed] [Google Scholar]
  18. Laskey R. A., Mills A. D. Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975 Aug 15;56(2):335–341. doi: 10.1111/j.1432-1033.1975.tb02238.x. [DOI] [PubMed] [Google Scholar]
  19. Mangel W. F., Hewick R. M., Bayley S. T., Wheeler T., Harvey R., Waterfield M. D., Smith A. E. Polyoma virus complementary RNA directs the in vitro synthesis of capsid proteins VP1 and VP2. J Virol. 1978 Feb;25(2):570–578. doi: 10.1128/jvi.25.2.570-578.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Oppermann H., Koch G. Individual translational efficiencies of SV40 and cellular mRNAs. Arch Virol. 1976;52(1-2):123–134. doi: 10.1007/BF01317871. [DOI] [PubMed] [Google Scholar]
  21. Prives C. L., Aviv H., Gilboa E., Revel M., Winocour E. The cell-free translation of SV40 messenger RNA. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):309–316. doi: 10.1101/sqb.1974.039.01.041. [DOI] [PubMed] [Google Scholar]
  22. Reddy V. B., Thimmappaya B., Dhar R., Subramanian K. N., Zain B. S., Pan J., Ghosh P. K., Celma M. L., Weissman S. M. The genome of simian virus 40. Science. 1978 May 5;200(4341):494–502. doi: 10.1126/science.205947. [DOI] [PubMed] [Google Scholar]
  23. Rozenblatt S., Mulligan R. C., Gorecki M., Roberts B. E., Rich A. Direct biochemical mapping of eukaryotic viral DNA by means of a linked transcription-translation cell-free system. Proc Natl Acad Sci U S A. 1976 Aug;73(8):2747–2751. doi: 10.1073/pnas.73.8.2747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Smith A. E., Kamen R., Mangel W. F., Shure H., Wheeler T. Location of the sequences coding for capsid proteins VP1 and VP2 on polyoma virus DNA. Cell. 1976 Nov;9(3):481–487. doi: 10.1016/0092-8674(76)90093-3. [DOI] [PubMed] [Google Scholar]
  25. Smith A. E., Marcker K. A. Cytoplasmic methionine transfer RNAs from eukaryotes. Nature. 1970 May 16;226(5246):607–610. doi: 10.1038/226607a0. [DOI] [PubMed] [Google Scholar]
  26. Wheeler T., Bayley S. T., Harvey R., Crawford L. V., Smith A. E. Cell-free synthesis of polyoma virus capsid proteins VP1 and VP2. J Virol. 1977 Jan;21(1):215–224. doi: 10.1128/jvi.21.1.215-224.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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