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. 1978 Oct;28(1):6–13. doi: 10.1128/jvi.28.1.6-13.1978

Persistent Infection of L Cells with Vesicular Stomatitis Virus: Evolution of Virus Populations

Julius S Youngner 1, Olivia T Preble 1, Elaine V Jones 1
PMCID: PMC354241  PMID: 212614

Abstract

A previous report (Youngner et al., J. Virol. 19:90-101, 1976) documented that noncytocidal persistent infection can be established with wild-type vesicular stomatitis virus (VSV) in mouse L cells at 37°C and that a rapid selection of RNA−, group I temperature-sensitive (ts) mutants consistently occurs in this system. To assess the selective advantage of the RNA−ts phenotype, evolution of the virus population was studied in persistent infections initiated in L cells by use of VSV ts 0 23 and ts 0 45, RNA+ mutants belonging to complementation groups III and V. In L cells persistently infected with ts 0 23, the ts RNA+ virus population was replaced gradually by viruses which had a ts RNA− phenotype. VSV ts 0 45 (V) has another marker in addition to reduced virus yield at 39.5°C: a defective protein (G) which renders virion infectivity heat labile at 50°C. Persistent infections initiated with this virus (ts, heat labile, RNA+) evolved into a virus population which was ts, heat resistant, and RNA−. These findings suggest that the ts phenotype itself is not sufficient to stabilize the VSV population in persistently infected L cells and also indicate that the ts RNA− phenotype may have a unique selective advantage in this system. In addition to the selection of ts RNA− mutants, other mechanisms which also might operate in the maintenance of persistent VSV infections of L cells were explored. Whereas defective-interfering particles did not seem to mediate the carrier state, evidence was obtained that interferon may play a role in the regulation of persistent infections of L cells with VSV.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BELLETT A. J., COOPER P. D. Some properties of the transmissible interfering component of vesicular stomatitis virus preparations. J Gen Microbiol. 1959 Dec;21:498–509. doi: 10.1099/00221287-21-3-498. [DOI] [PubMed] [Google Scholar]
  2. Flamand A. Etude génétique du virus de la stomatite vésiculaire: classement de mutants thermosensibles spontanés en groupes de complémentation. J Gen Virol. 1970 Sep;8(3):187–195. doi: 10.1099/0022-1317-8-3-187. [DOI] [PubMed] [Google Scholar]
  3. Holland J. J., Villarreal L. P., Breindl M. Factors involved in the generation and replication of rhabdovirus defective T particles. J Virol. 1976 Mar;17(3):805–815. doi: 10.1128/jvi.17.3.805-815.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Holland J. J., Villarreal L. P. Purification of defective interfering T particles of vesicular stomatitis and rabies viruses generated in vivo in brains of newborn mice. Virology. 1975 Oct;67(2):438–449. doi: 10.1016/0042-6822(75)90445-6. [DOI] [PubMed] [Google Scholar]
  5. Inglot A. D., Albin M., Chudzio T. Persistent infection of mouse cells with Sindbis virus: role of virulence of strains, auto-interfering particles and interferon. J Gen Virol. 1973 Jul;20(1):105–110. doi: 10.1099/0022-1317-20-1-105. [DOI] [PubMed] [Google Scholar]
  6. Nishiyama Y. Studies of L cells persistently infected with VSV: factors involved in the regulation of persistent infection. J Gen Virol. 1977 May;35(2):265–279. doi: 10.1099/0022-1317-35-2-265. [DOI] [PubMed] [Google Scholar]
  7. Potter K. N., Stewart R. B. Comparison of vesicular stomatitis virus defective interfering particle synthesis in chick embryo and L cells. Can J Microbiol. 1976 Oct;22(10):1458–1463. doi: 10.1139/m76-216. [DOI] [PubMed] [Google Scholar]
  8. Ramseur J. M., Friedman R. M. Prolonged infection of L cells with vesicular stomatitis virus. Defective interfering forms and temperature-sensitive mutants as factors in the infection. Virology. 1978 Mar;85(1):253–261. doi: 10.1016/0042-6822(78)90429-4. [DOI] [PubMed] [Google Scholar]
  9. Schnitzer T. J., Richardson L. S., Chanock R. M. Growth and genetic stability of the ts-1 mutant of respiratory syncytial virus at restrictive temperatures. J Virol. 1976 Feb;17(2):431–438. doi: 10.1128/jvi.17.2.431-438.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sekellick M. J., Marcus P. I. Persistent infection. I Interferon-inducing defective-interfering particles as mediators of cell sparing: possible role in persistent infection by vesicular stomatitis virus. Virology. 1978 Mar;85(1):175–186. doi: 10.1016/0042-6822(78)90422-1. [DOI] [PubMed] [Google Scholar]
  11. Stanwick T. L., Hallum J. V. Role of interferon in six cell lines persistently infected with rubella virus. Infect Immun. 1974 Oct;10(4):810–815. doi: 10.1128/iai.10.4.810-815.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Youngner J. S., Dubovi E. J., Quagliana D. O., Kelly M., Preble O. T. Role of temperature-sensitive mutants in persistent infections initiated with vesicular stomatitis virus. J Virol. 1976 Jul;19(1):90–101. doi: 10.1128/jvi.19.1.90-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Youngner J. S., Quagliana D. O. Temperature-sensitive mutants isolated from hamster and canine cell lines persistently infected with Newcastle disease virus. J Virol. 1975 Nov;16(5):1332–1336. doi: 10.1128/jvi.16.5.1332-1336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J Bacteriol. 1966 Oct;92(4):862–868. doi: 10.1128/jb.92.4.862-868.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ter Meulen V., Martin S. J. Genesis and maintenance of a persistent infection by canine distemper virus. J Gen Virol. 1976 Sep;32(3):431–440. doi: 10.1099/0022-1317-32-3-431. [DOI] [PubMed] [Google Scholar]

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