Abstract
This report presents the first experimental demonstration, to our knowledge, of benchtop polychromatic cone-beam x-ray fluorescence computed tomography (XFCT) for a simultaneous determination of the spatial distribution and amount of gold nanoparticles (GNPs) within small-animal-sized objects. The current benchtop experimental setup successfully produced XFCT images accurately showing the regions containing small amount of GNPs (on the order of 0.1 mg) within a 3-cm diameter plastic phantom. In particular, the performance of the current XFCT setup was improved remarkably (e.g., at least a factor of 3 reduction in XFCT scan time) using a tin-filtered polychromatic beam in comparison with a lead-filtered beam. The results of this study strongly suggest the current benchtop XFCT configuration can be made practical with a few modifications such as the deployment of array detectors, while meeting realistic constraints on x-ray dose, scan time, and image resolution for routine pre-clinical in-vivo imaging with GNPs.
1. Introduction
Some notable applications with gold nanoparticles (GNPs) have emerged for cancer imaging (Sokolov et al., 2003; Hainfeld et al., 2006; Qian et al., 2008; Li et al., 2010), radiation therapy (Hainfeld et al., 2004; Cho, 2005; Cho et al., 2009; Jones et al., 2010; Hainfeld et al., 2008; Hainfeld et al., 2010), and thermal therapy (Hirsch et al., 2003; Diagaradjane et al., 2008; von Maltzahn et al., 2009; Krishnan et al., 2010). Many of these approaches are currently undergoing pre-clinical testing using small animal models. One of the key tasks during pre-clinical studies is to determine the biodistribution of GNPs, and this task is typically handled by somewhat cumbersome and time-consuming ex-vivo analysis after sacrificing animals. Ideally, it is desirable to determine the biodistribution of GNPs in vivo using a tool capable of identifying both the spatial distribution and amount of GNPs simultaneously. This will also enable GNP-based pre-clinical molecular imaging of cancer and other diseases, especially with bioconjugated GNPs.
In principle, x-ray fluorescence computed tomography (XFCT) can be a powerful quantitative imaging tool for small samples containing high atomic number (Z) materials such as iodine, gadolinium, and gold. Especially in the case of gold, the relatively low attenuation of K-shell x-ray fluorescence photons (~69 and 78 keV) in soft tissue may enable XFCT of small animals. Therefore, XFCT appears to be particularly well suited for the determination of GNP biodistribution in vivo. Monochromatic x-ray sources are highly desirable for XFCT because their energy can easily be tuned to maximize the fluorescence-to-background ratio for the interrogated material (Boisseau, 1986; Cesareo and Mascarenhas, 1989; Hogan et al., 1991; La Rivière, 2004; La Riviere et al., 2009; Schroer, 2001; Simionovici et al., 2000; Yuasa et al., 1997). Synchrotron x-ray sources are excellent for this purpose but they are clearly impractical for most biomedical research laboratories.
For routine benchtop applications, an implementation of XFCT using a conventional polychromatic source is much more realistic but far more challenging than that using a monochromatic source primarily due to the polychromatic nature of x-ray source resulting in less efficient fluorescence x-ray production/detection (e.g., leading to much lower fluorescence-to-background ratio). These challenges often resulted in a pessimistic outlook about a benchtop XFCT system for in-vivo imaging (von Busch et al., 2005). Recently, however, we showed the possibility for simultaneously determining GNP location and concentration using a benchtop XFCT setup with a polychromatic x-ray source (i.e., 110 kVp) under the pencil-beam geometry (Cheong et al., 2010). We also demonstrated in a more recent Monte Carlo (MC) study that the inherent limitations of the pencil-beam geometry, such as long scan time and excessive x-ray dose, can be overcome using a fan/cone-beam geometry (Jones and Cho, 2011). Nevertheless, the approaches and results from this MC study need to be validated by an experimental study in order to fully establish the feasibility of a polychromatic cone-beam XFCT setup.
Therefore, the primary goals of the current experimental study were to build a benchtop polychromatic cone-beam XFCT system as proposed in our previous MC study and to assess its performance via phantom studies in view of realistic constraints on x-ray dose, scan time, and image resolution for XFCT of small animals injected with GNPs. After the initial construction and testing, we also investigated the possibility to further enhance the performance of our benchtop XFCT system by a simple modification adopting a tin-filtered polychromatic x-ray spectrum instead of a lead-filtered one. Overall, the results and findings from this experimental study provide much more realistic insight, compared to our previous pencil beam and MC studies, about the challenges and promises for building a practical and useful benchtop XFCT device for in-vivo imaging with GNPs.
2. Methods and Materials
2.1. Imaging Phantoms
Samples were prepared based on realistic tumor/blood gold concentrations achievable in vivo (Chang et al., 2008; Hainfeld et al., 2004): namely, 0.5, 1.0, and 2.0% gold by weight (wt.), mimicking a practical small-animal XFCT scenario. Roughly cylindrical tubes (6 mm in diameter) were filled with saline solution, and commercially available 1.9 nm diameter GNPs (Aurovist™, Nanoprobes Inc) were added. The GNP-loaded tubes were then inserted into a small-animal-sized polymethyl methacrylate (PMMA) cylindrical phantom 3 cm in diameter and 5 cm in height. The GNP-loaded tubes contained 1.6–6.5 mg of GNPs (corresponding to 0.5 to 2.0 wt. % of GNP concentrations within the entire tubes).
2.2. Benchtop XFCT Setup
To acquire images showing the GNP-loaded regions with associated gold concentrations, each phantom was irradiated with a microfocus x-ray source (L9631, Hamamatsu Photonics, Inc) operating at tube potential of 105 kVp and tube current of 400 µA. The source had a nominal focal spot size less than 100 µm and an emission angle of 62°. As shown in Fig. 1, the beam was collimated by a conical hole through a 5 cm-thick lead block. The angle of the conical hole was 11.4°, with front and back hole diameters of 1 and 2 cm, respectively. The center of the phantom was roughly 8 cm from the collimator surface (14.5 cm from exit window of source), yielding a beam with a diameter just exceeding the 3-cm phantom diameter.
As shown previously (Cheong et al., 2010; Jones and Cho, 2011), significant hardening or quasi-monochromatization of polychromatic x-ray spectrum was necessary to implement XFCT with an ordinary x-ray source. By adopting x-ray filters made of lead (Pb: 1.0 mm) and tin (Sn: 0.9 mm), therefore, we achieved a reasonable suppression of those x-ray photons below the K-absorption edge of gold (80.7 keV) that would not produce gold K-shell fluorescence photons (Fig. 2a). Lead has been used in our previous benchtop XFCT studies; however, we have also used tin as a filter material in the current study because tin has much lower absorption above 80 keV (where lead K-shell absorption is peaked) than lead, yielding a higher number of gold Kα fluorescence per primary photon. In fact, the performance of our benchtop XFCT setup has improved remarkably with the filter made of tin, as shown later in this report.
Gold K-shell fluorescence photons were detected by two identical thermoelectrically cooled cadmium telluride (CdTe) detectors (XR-100T-CdTe, Amptek Inc) (see Fig. 1). A digital pulse processor and multichannel analyzer (PX4, Amptek Inc) interfaced between the detectors and a personal computer, which collected, stored, and analyzed the results. Measured photon spectra were corrected for the energy-dependent efficiency of the current CdTe detector. Since the measured spectrum of photons emitted from the phantom was overwhelmingly dominated by Compton scatter as shown in Fig. 2(b), the detectors were placed behind lead pinhole collimators. Each detector was positioned behind a 4 cm-thick lead pinhole collimator with an aperture diameter of 2.5 mm, which was placed 0.75 cm away from the phantom surface. Note the detector collimation is referred to as “pinhole” collimation here for convenience despite its relatively larger aperture size. Only a fraction of the GNP-loaded tubes was ever in view of the detector, since the height of the tubes (>2 cm) is much larger than the pinhole diameter (2.5 mm). For the 0.5 wt. % GNP tube, this translated into less than 0.5 mg of GNPs visible in the best-case scenario.
2.3. Data Acquisition and Image Reconstruction
As shown in Fig. 1, the view of each detector was along the x-axis, at a 90° angle to the central axis of the beam (i.e., z-axis). The system remained stationary for the acquisition of each projection, and then the phantom was rotated about its central axis by 6° (along the angular direction θ as shown in Fig. 1). This process was repeated for 60 projections, corresponding to 360° coverage of the phantom. In order to simulate an 11-pixel serial detector, each CdTe detector was translated along the z-axis (e.g., to position d as shown in Fig. 1) in equal intervals (3 mm) after the completion of a full 360° rotation. In order to increase the data collection rate, as well as the spatial resolution, the two detectors were slightly offset (1.5 mm) along the z-axis.
Data were acquired as a series of at least three sets of 60 projections, allowing the reconstruction of images corresponding to three different levels of x-ray exposure per projection (e.g., 1, 2, and 3 minute exposure per projection). For each sinogram element S(θ, d), the gold fluorescence peak height was extracted from the Compton scatter background by fitting a 3rd degree polynomial to the measured data points on either side of the gold Kα channels, and subtracting out the interpolated values for the scatter background. These signals were assembled to form a sinogram of gold fluorescence signal with respect to position and angle. More details about this procedure can be found elsewhere (Cheong et al., 2010). Using the sinograms created during the data acquisition, an image of the GNP distribution inside the phantom was reconstructed by applying an approach developed in our previous Monte Carlo study (Jones and Cho, 2011) following the Maximum Likelihood Expectation Maximization (ML-EM) iterative reconstruction algorithm (Shepp and Vardi, 2007; Lange and Carson, 1984; Cherry et al., 2003), taking into account various factors affecting the fluorescence photon emission and detection probability. A measured attenuation map of the phantom was unavailable from the current experimental setup due to the absence of a transmission detector. As a result, attenuation coefficients for gold and PMMA were taken as priori knowledge.
2.4. X-ray Dose Measurements
In order to measure the x-ray dose delivered during XFCT scanning, ion chamber measurements were performed using a standard Farmer-type ionization chamber (PTW N30013, PTW Freiburg GmbH) and an electrometer (Max 4000), Standard Imaging, Inc). The dose measurement geometry was identical to the irradiation geometry for the phantom used for XFCT scanning via the utilization of a PMMA phantom constructed to hold the ion chamber. The x-ray beam matched the beam used in the corresponding imaging scenario in terms of power/filtration used. The AAPM TG-61 protocol (Coffey et al., 2001) was applied to determine the x-ray dose rates from ionization chamber measurements.
3. Results
The measured sinograms for the two most representative cases are shown in Fig. 3. Using these data, the images of GNP location and concentration were reconstructed (Fig. 4). As shown, using the Sn-filter, one minute of exposure at each projection was sufficient to produce an XFCT image significantly better than that reconstructed using the data acquired from three minute exposure time per projection with the Pb-filter. In terms of scan time alone, this means the Sn-filter yielded at least a factor of 3 reduction over the Pb-filter. All three GNP-loaded columns were well defined in each image (with some regions of low-intensity noise). Fig. 5 shows the relationship between signal intensity and GNP concentration in each image. Each data point represents the mean intensity in each region of the image in Fig. 4 (i.e., regions denoted as 0.5%, 1%, and 2 %). The relative concentration of GNPs was accurately determined in both images, showing a good linear relationship between gold concentration and signal intensity.
The measured dose rates at the center of the phantom were 1.7 mGy/min for the Pb-filtered beam and 3.4 mGy/min for the Sn-filtered beam. The Sn-filtered beam delivers twice the dose rate of the Pb-filtered beam, but acquires a better image in 1/3 of the time, making the tin filter at least 50% more effective for XFCT imaging per unit dose.
4. Discussion
With a proper choice of x-ray filter (i.e., tin), the current XFCT setup appears to require no more than 1 minute of data acquisition time at each projection angle in order to produce reconstructed XFCT images showing all three GNP-loaded tubes within a small-animal-sized PMMA phantom. This means, with the deployment of two 11-pixel CdTe array detectors to the current XFCT setup, the total scan time would be just about an hour, presumably acceptable for unconventional in-vivo imaging tasks such as the determination of GNP biodistribution. Thus, it is foreseeable to perform a practical benchtop XFCT scanning of small animals, at least those animals comparable in their sizes to the current imaging phantom (e.g., transgenic nude mice). A similar argument can also be made for x-ray dose. Specifically, the use of 11-pixel CdTe detectors would reduce the total x-ray dose by a factor of 11, resulting in the total x-ray dose required for XFCT imaging using the current methodology to be roughly 20 cGy. This is clearly much less than the general LD50 (50% Lethal Dose) for mice of about 7 Gy (Hall and Giaccia, 2006), and possibly less than typical ranges of x-ray doses delivered during micro-CT scanning of small animals (Boone et al., 2004; Obenaus and Smith, 2004).
A few more modifications of the current XFCT setup and approaches can be made for further improvement in image quality, detection limit, and scan time (or x-ray dose). The use of a two-dimensional (2-D) array detector (Schlomka et al., 2008) with more pixel elements than the currently simulated 11 pixels in 2-D space would significantly improve the resolution of XFCT images at the same x-ray dose. The scan time can be easily reduced further because it is not essential to perform a full 360° scanning in order to reconstruct XFCT images (Jones and Cho, 2011; La Riviere et al., 2007; Jones, 2011). Moreover, it is possible that the amount of data required to reconstruct an acceptable image could be further reduced by using a more sophisticated gold fluorescence peak extraction algorithm, or by using a detector with a higher energy resolution at the energies considered (65–70 keV). Besides, the current experimental setup, especially the source and detector collimators, may also be further optimized to improve the detection of fluorescence photons while reducing the production of unwanted scattered photons.
Additionally, the scan time and detection limit could be further reduced by optimizing the incident x-ray spectrum using higher x-ray tube potentials/currents and/or beam filtration techniques that improve the ratio of photons above/below the K-absorption edge of gold (80.7 keV). To illustrate this possibility in a more quantitative fashion, an extra set of fluorescence and dose measurements was also made in this investigation using the same beam geometry and phantoms as described earlier. As shown in Fig. 6, the ratio of gold fluorescence signal to delivered dose increases exponentially with tin filter thickness (i.e., hardness of the incident photon spectrum). However, the scan time to produce the same magnitude of gold fluorescence signal (from the 2 wt. % GNP region) also increases with tin filter thickness, at a much higher rate, under the current x-ray tube power setting (i.e., 105 kVp and 400 µA). Thus, it is imperative to use a higher power x-ray tube for XFCT scanning in order to fully take advantage of an increased fluorescence signal-to-dose ratio from the use of thicker filters. The current results will be useful for the determination of an optimum combination of filter thickness and x-ray power setting. In summary, with the implementation of all or part of the aforementioned modifications for the current XFCT setup, benchtop XFCT will ultimately be available for routine in-vivo imaging, offering a new and unique option for pre-clinical multimodal molecular imaging of various diseases in conjunction with bioconjugated GNPs and simultaneous micro-CT in the same platform.
5. Conclusions
In the current experimental investigation, we demonstrated the feasibility to image low local concentrations of GNPs (e.g., 0.5 mg within the region seen by a 2.5 mm-diameter pin-hole collimator) in small-animal-sized objects (e.g., 3 cm in diameter and 5 cm in height) by a benchtop XFCT setup utilizing a polychromatic cone-beam x-ray source in the diagnostic energy range. The use of Sn-filtered polychromatic beam, in comparison with the Pb-filtered beam, improved the performance of the current experimental XFCT setup remarkably (e.g., at least a factor of 3 reduction in XFCT scan time), strongly suggesting the feasibility to build a practical benchtop XFCT device based on the current setup with a few modifications. Considering the results shown in this study, it can be projected without loss of generality that, using array detectors, the current experimental setup would be capable of producing useful XFCT images of small animals injected with GNPs with a total scan time (< 1 hour) and a tissue dose (~ 20 cGy) acceptable for routine pre-clinical in-vivo imaging.
Acknowledgments
This investigation was supported by Georgia Research Alliance and Georgia Institute of Technology faculty startup funds and in part by NIH/NCI grant R01CA155446. Dr. Karellas was supported in part by NIH/NCI grant R21CA134128. The contents are solely the responsibility of the authors and do not represent the official views of the NCI or NIH. The authors acknowledge Dr. Chris Wang at Georgia Tech for helpful discussion and kindly sharing an electrometer during the current investigation. The authors are also grateful to Dr. John Oshinski and Dr. Eric Elder at Emory University for constructive criticisms on the current investigation.
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