Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Jan;15(1):16–21. doi: 10.1128/jvi.15.1.16-21.1975

Bacteriophage transformation of PBS2 in Bacillus subtilis.

M B Herrington, I Takahashi
PMCID: PMC354411  PMID: 803565

Abstract

Transformation of temperature-sensitive mutants of bacteriophage PBS2 for Bacillus subtilis was demonstrated. The number of transformants was linearly related to the concentration of DNA within a range of 0.01 to 1 mug/ml. No transformants were obtained when the DNA was pretreated with DNase. PBS2 DNA sheared to approximately 1% of the total chromosome length was centrifuged in Cs2SO4-Hg gradients to fractionate the DNA according to the base composition. Transformation experiments carried out with the fractionated DNA indicated the possibility of determining the base composition of different regions of the phage chromosome.

Full text

PDF
16

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. GREEN D. M. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. doi: 10.1016/s0022-2836(64)80065-6. [DOI] [PubMed] [Google Scholar]
  2. Herrington M. B., Takahashi I. Mutagenesis of bacteriophage PBS 2. Mutat Res. 1973 Nov;20(2):275–278. doi: 10.1016/0027-5107(73)90198-x. [DOI] [PubMed] [Google Scholar]
  3. Hunter B. I., Yamagishi H., Takahashi I. Molecular weight of bacteriophage PBS 1 deoxyribonucleic acid. J Virol. 1967 Aug;1(4):841–842. doi: 10.1128/jvi.1.4.841-842.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Nester E W, Schafer M, Lederberg J. Gene Linkage in DNA Transfer: A Cluster of Genes Concerned with Aromatic Biosynthesis in Bacillus Subtilis. Genetics. 1963 Apr;48(4):529–551. doi: 10.1093/genetics/48.4.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Rosenberg B. H., Sirotnak F. M., Cavalieri L. F. ON THE SIZE OF GENETIC DETERMINANTS IN PNEUMOCOCCUS AND THE NATURE OF THE VARIABLES INVOLVED IN TRANSFORMATION. Proc Natl Acad Sci U S A. 1959 Feb;45(2):144–156. doi: 10.1073/pnas.45.2.144. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Skalka A., Burgi E., Hershey A. D. Segmental distribution of nucleotides in the DNA of bacteriophage lambda. J Mol Biol. 1968 May 28;34(1):1–16. doi: 10.1016/0022-2836(68)90230-1. [DOI] [PubMed] [Google Scholar]
  7. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. TAKAHASHI I. Genetic transduction in Bacillus subtilis. Biochem Biophys Res Commun. 1961 Jun 28;5:171–175. doi: 10.1016/0006-291x(61)90104-8. [DOI] [PubMed] [Google Scholar]
  9. TAKAHASHI I. LOCALIZATION OF SPORE MARKERS ON THE CHROMOSOME OF BACILLUS SUBTILIS. J Bacteriol. 1965 Apr;89:1065–1067. doi: 10.1128/jb.89.4.1065-1067.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. TAKAHASHI I., MARMUR J. Replacement of thymidylic acid by deoxyuridylic acid in the deoxyribonucleic acid of a transducing phage for Bacillus subtilis. Nature. 1963 Feb 23;197:794–795. doi: 10.1038/197794a0. [DOI] [PubMed] [Google Scholar]
  11. TAKAHASHI I. TRANSDUCTION OF SPOROGENESIS IN BACILLUS SUBTILIS. J Bacteriol. 1965 Feb;89:294–298. doi: 10.1128/jb.89.2.294-298.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. TAKAHASHI I. Transducing phages for Bacillus subtilis. J Gen Microbiol. 1963 May;31:211–217. doi: 10.1099/00221287-31-2-211. [DOI] [PubMed] [Google Scholar]
  13. Yamagishi H. Nucleotide distribution in the DNA of Escherichia coli. J Mol Biol. 1970 May 14;49(3):603–608. doi: 10.1016/0022-2836(70)90284-6. [DOI] [PubMed] [Google Scholar]
  14. Yamagishi H., Takahashi I. Heterogeneity in nucleotide composition of Bacillus subtilis DNA. J Mol Biol. 1971 Apr 28;57(2):369–371. doi: 10.1016/0022-2836(71)90355-x. [DOI] [PubMed] [Google Scholar]
  15. Yamagishi H., Takahashi I. Transducing particles of PBS 1. Virology. 1968 Dec;36(4):639–645. doi: 10.1016/0042-6822(68)90194-3. [DOI] [PubMed] [Google Scholar]
  16. va de POL J. H., VELDHUISEN G., COHEN J. A. Phage transformation: a new criterium for the biological activity of bacteriophage DNA. Biochim Biophys Acta. 1961 Apr 1;48:417–418. doi: 10.1016/0006-3002(61)90501-7. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES