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. 1975 Jan;15(1):41–49. doi: 10.1128/jvi.15.1.41-49.1975

Screening procedure for complementation-dependent mutants of vesicular stomatitis virus.

C W Rettenmier, R Dumont, D Baltimore
PMCID: PMC354415  PMID: 163345

Abstract

To isolate new types of vesicular stomatitis virus (VSV) mutants, a four-stage screen was developed which identifies and characterizes mutants capable of complementing the defect in the VSV temperature-sensitive mutant tsG11. Two types of mutants of VSV, Indiana serotype, have been found by using the screen; they are new temperature-sensitive mutants which are, of necessity, not in complementation group I and mutants which do not produce plaques under conditions of single infection at 31 C (the normal permissive temperature) and are, therefore, called complementation-dependent mutants. The newly isolated, temperature-sensitive mutants fall into three complementation groups, two of which are congruent with known complementation groups; the newly identified group extends to six the number of complementation groups of VSV Indiana. The nature of the complementation-dependent mutants has not been established, but one was shown to not contain a significant deletion in its nucleic acid.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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