Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Jan;15(1):81–89. doi: 10.1128/jvi.15.1.81-89.1975

Lifetimes of mRNA molecules directing the synthesis of viral proteins in herpes simplex virus-infected cells.

R L Ward, J G Stevens
PMCID: PMC354420  PMID: 46283

Abstract

Possible variations in the functional lifetimes of herpes simplex virus type 1 mRNA molecules in infected HeLa cells were studied. As shown by the rate of decrease of radioactive amino acid incorporation into viral proteins after the addition of actinomycin, the average lifetime of early viral mRNA's are shorter than those for the late messenger species. In addition, when the viral proteins made after the addition of actinomycin were further analyzed by gel electrophoresis, it was found that messengers for individual viral proteins translated within the early or late time period also had some differences in their functional lifetimes. These results indicate that the synthesis of herpes simplex virus type 1 proteins during the replicative cycle is regulated in part by mechanisms controlling the functional lifetimes of viral mRNA's.

Full text

PDF
81

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Craig N., Kelley D. E., Perry R. P. Lifetime of the messenger RNA's which code for ribosomal proteins in L-cells. Biochim Biophys Acta. 1971 Sep 24;246(3):493–498. doi: 10.1016/0005-2787(71)90786-6. [DOI] [PubMed] [Google Scholar]
  2. Fan H., Penman S. Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis. J Mol Biol. 1970 Jun 28;50(3):655–670. doi: 10.1016/0022-2836(70)90091-4. [DOI] [PubMed] [Google Scholar]
  3. Godchaux W., 3rd, Adamson S. D., Herbert E. Effects of cycloheximide on polyribosome function in reticulocytes. J Mol Biol. 1967 Jul 14;27(1):57–72. doi: 10.1016/0022-2836(67)90351-8. [DOI] [PubMed] [Google Scholar]
  4. Goldstein E. S., Penman S. Regulation of protein synthesis in mammalian cells. V. Further studies on the effect of actinomycin D on translation control in HeLa cells. J Mol Biol. 1973 Oct 25;80(2):243–254. doi: 10.1016/0022-2836(73)90170-8. [DOI] [PubMed] [Google Scholar]
  5. Honess R. W., Roizman B. Proteins specified by herpes simplex virus. XI. Identification and relative molar rates of synthesis of structural and nonstructural herpes virus polypeptides in the infected cell. J Virol. 1973 Dec;12(6):1347–1365. doi: 10.1128/jvi.12.6.1347-1365.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jungwirth C., Joklik W. K. Studies on "early" enzymes in HeLa cells infected with vaccinia virus. Virology. 1965 Sep;27(1):80–93. doi: 10.1016/0042-6822(65)90145-5. [DOI] [PubMed] [Google Scholar]
  7. Kates J. R., McAuslan B. R. Relationship between protein synthesis and viral deoxyribonucleic acid synthesis. J Virol. 1967 Feb;1(1):110–114. doi: 10.1128/jvi.1.1.110-114.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. MCAUSLAN B. R. THE INDUCTION AND REPRESSION OF THYMIDINE KINASE IN THE POXVIRUS-INFECTED HELA CELL. Virology. 1963 Nov;21:383–389. doi: 10.1016/0042-6822(63)90199-5. [DOI] [PubMed] [Google Scholar]
  9. McAuslan B. R., Herde P., Pett D., Ross J. Nucleases of virus-infected animal cells. Biochem Biophys Res Commun. 1965 Sep 8;20(5):586–591. doi: 10.1016/0006-291x(65)90439-0. [DOI] [PubMed] [Google Scholar]
  10. Penman S., Scherrer K., Becker Y., Darnell J. E. POLYRIBOSOMES IN NORMAL AND POLIOVIRUS-INFECTED HELA CELLS AND THEIR RELATIONSHIP TO MESSENGER-RNA. Proc Natl Acad Sci U S A. 1963 May;49(5):654–662. doi: 10.1073/pnas.49.5.654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REICH E., FRANKLIN R. M., SHATKIN A. J., TATUM E. L. Effect of actinomycin D on cellular nucleic acid synthesis and virus production. Science. 1961 Aug 25;134(3478):556–557. doi: 10.1126/science.134.3478.556. [DOI] [PubMed] [Google Scholar]
  12. SHATKIN A. J. ACTINOMYCIN AND THE DIFFERENTIAL SYNTHESIS OF REOVIRUS AND L CELL RNA. Biochem Biophys Res Commun. 1965 May 3;19:506–510. doi: 10.1016/0006-291x(65)90154-3. [DOI] [PubMed] [Google Scholar]
  13. Sebring E. D., Salzman N. P. Metabolic properties of early and late vaccinia virus messenger ribonucleic acid. J Virol. 1967 Jun;1(3):550–558. doi: 10.1128/jvi.1.3.550-558.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Singer R. H., Penman S. Messenger RNA in HeLa cells: kinetics of formation and decay. J Mol Biol. 1973 Aug 5;78(2):321–334. doi: 10.1016/0022-2836(73)90119-8. [DOI] [PubMed] [Google Scholar]
  15. Singer R. H., Penman S. Stability of HeLa cell mRNA in actinomycin. Nature. 1972 Nov 10;240(5376):100–102. doi: 10.1038/240100a0. [DOI] [PubMed] [Google Scholar]
  16. Ward R. L., Stevens J. G. Effect of cytosine arabinoside on viral-specific protein synthesis in cells infected with herpes simplex virus. J Virol. 1975 Jan;15(1):71–80. doi: 10.1128/jvi.15.1.71-80.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. ZIMMERMAN E. F., HEETER M., DARNELL J. E. RNA synthesis in poliovirus-infected cells. Virology. 1963 Mar;19:400–408. doi: 10.1016/0042-6822(63)90080-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES