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. 1975 Jan;15(1):173–181. doi: 10.1128/jvi.15.1.173-181.1975

Characterization of human papovavirus BK DNA.

P M Howley, M F Mullarkey, K K Takemoto, M A Martin
PMCID: PMC354432  PMID: 163342

Abstract

The DNA of the BK virus (BKV) human papovavirus was found to be heterogeneous, consisting of at least four discrete species of DNA. Only the largest of these four species, BKV DNA (i), which has a molecular weight calculated to be 96% that of simian virus 40 (SV40) DNA, was infectious. Homogeneous preparations of BKV DNA were obtained, however, from virions purified after low multiplicity infections of human embryonic kidney cells. BKV DNA (i) was shown to contain a single R-Eco RI and four R-Hind cleavage sites. The R-Eco RI site was localized in the largest R-Hind cleavage fragment. Radiolabeled BKV DNA reassociated slightly faster than SV40 DNA; 20 to 30% polynucleotide sequence homology was demonstrated between the genomes of BKV and SV40 when the reaction was monitored by chromatography on hydroxyapatite.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brockman W. W., Lee T. N., Nathans D. The evolution of new species of viral DNA during serial passage of simian virus 40 at high multiplicity. Virology. 1973 Aug;54(2):384–397. doi: 10.1016/0042-6822(73)90151-7. [DOI] [PubMed] [Google Scholar]
  2. Coleman D. V., Gardner S. D., Field A. M. Human polyomavirus infection in renal allograft recipients. Br Med J. 1973 Aug 18;3(5876):371–375. doi: 10.1136/bmj.3.5876.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Fareed G. C., Garon G. F., Salzman N. P. Origin and direction of simian virus 40 deoxyribonucleic acid replication. J Virol. 1972 Sep;10(3):484–491. doi: 10.1128/jvi.10.3.484-491.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gardner S. D., Field A. M., Coleman D. V., Hulme B. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet. 1971 Jun 19;1(7712):1253–1257. doi: 10.1016/s0140-6736(71)91776-4. [DOI] [PubMed] [Google Scholar]
  5. Gardner S. D. Prevalence in England of antibody to human polyomavirus (B.k.). Br Med J. 1973 Jan 13;1(5845):77–78. doi: 10.1136/bmj.1.5845.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gelb L. D., Kohne D. E., Martin M. A. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. doi: 10.1016/0022-2836(71)90123-9. [DOI] [PubMed] [Google Scholar]
  7. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  8. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  9. Lavi S., Winocour E. Acquisition of sequences homologous to host deoxyribonucleic acid by closed circular simian virus 40 deoxyribonucleic acid. J Virol. 1972 Feb;9(2):309–316. doi: 10.1128/jvi.9.2.309-316.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin M. A., Gelb L. D., Fareed G. C., Milstien J. B. Reassortment of simian virus 40 DNA during serial undiluted passage. J Virol. 1973 Oct;12(4):748–757. doi: 10.1128/jvi.12.4.748-757.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martin M. A., Gelb L. D., Garon C., Takemoto K. K., Lee T. N., Sack G. H., Jr, Nathans D. Characterization of "heavy" and "light" SV40-like particles from a patient with PML. Virology. 1974 May;59(1):179–189. doi: 10.1016/0042-6822(74)90214-1. [DOI] [PubMed] [Google Scholar]
  12. McCutchan J. H., Pagano J. S. Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran. J Natl Cancer Inst. 1968 Aug;41(2):351–357. [PubMed] [Google Scholar]
  13. Morrow J. F., Berg P., Kelly T. J., Jr, Lewis A. M., Jr Mapping of simian virus 40 early functions on the viral chromosome. J Virol. 1973 Sep;12(3):653–658. doi: 10.1128/jvi.12.3.653-658.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mullarkey M. F., Hruska J. F., Takemoto K. K. Comparison of two human papovaviruses with simian virus 40 by structural protein and antigenic analysis. J Virol. 1974 May;13(5):1014–1019. doi: 10.1128/jvi.13.5.1014-1019.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Narayan O., Penney J. B., Jr, Johnson R. T., Herndon R. M., Weiner L. P. Etiology of progressive multifocal leukoencephalopathy. Identification of papovavirus. N Engl J Med. 1973 Dec 13;289(24):1278–1282. doi: 10.1056/NEJM197312132892405. [DOI] [PubMed] [Google Scholar]
  16. Osborn J. E., Robertson S. M., Padgett B. L., ZuRhein G. M., Walker D. L., Weisblum B. Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments. J Virol. 1974 Mar;13(3):614–622. doi: 10.1128/jvi.13.3.614-622.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Padgett B. L., Walker D. L., ZuRhein G. M., Eckroade R. J., Dessel B. H. Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet. 1971 Jun 19;1(7712):1257–1260. doi: 10.1016/s0140-6736(71)91777-6. [DOI] [PubMed] [Google Scholar]
  18. Penney J. B., Jr, Narayan O. Studies of the antigenic relationships of the new human papovaviruses by electron microscopy agglutination. Infect Immun. 1973 Aug;8(2):299–300. doi: 10.1128/iai.8.2.299-300.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Rozenblatt S., Lavi S., Singer M. F., Winocour E. Acquisition of sequences homologous to host DNA by closed circular simian virus 40 DNA. 3. Host sequences. J Virol. 1973 Sep;12(3):501–510. doi: 10.1128/jvi.12.3.501-510.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shah K. V., McCrumb F. R., Jr, Daniel R. W., Ozer H. L. Serologic evidence for a simian-virus-40-like infection of man. J Natl Cancer Inst. 1972 Feb;48(2):557–561. [PubMed] [Google Scholar]
  22. Shah K. V., Ozer H. L., Pond H. S., Palma L. D., Murphy G. P. SV40 neutralizing antibodies in sera of US residents without history of polio immunization. Nature. 1971 Jun 18;231(5303):448–449. doi: 10.1038/231448a0. [DOI] [PubMed] [Google Scholar]
  23. Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]
  24. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  25. Takemoto K. K., Mullarkey M. F. Human papovavirus, BK strain: biological studies including antigenic relationship to simian virus 40. J Virol. 1973 Sep;12(3):625–631. doi: 10.1128/jvi.12.3.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Trilling D. M., Axelrod D. Encapsidation of free host DNA by simian virus 40: a simian virus 40 pseudovirus. Science. 1970 Apr 10;168(3928):268–271. doi: 10.1126/science.168.3928.268. [DOI] [PubMed] [Google Scholar]
  27. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]
  28. Yoshiike K. Studies on DNA from low-density particles of SV40. I. Heterogeneous defective virions produced by successive undiluted passages. Virology. 1968 Mar;34(3):391–401. doi: 10.1016/0042-6822(68)90059-7. [DOI] [PubMed] [Google Scholar]
  29. Yoshike K. Studies on DNA from low-density particles of SV40. II. Noninfectious virions associated with a large-plaque variant. Virology. 1968 Mar;34(3):402–409. doi: 10.1016/0042-6822(68)90060-3. [DOI] [PubMed] [Google Scholar]

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