Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Jan;15(1):182–190. doi: 10.1128/jvi.15.1.182-190.1975

Comparative biophysical studies of hepatitis B antigen, subtypes adw and ayw.

R Chairez, F B Hollinger, J P Brunschwig, G R Dreesman
PMCID: PMC354433  PMID: 1113371

Abstract

Comparative biophysical and biochemical analyses were performed on purified preparations of hepatitis B antigen (HBs Ag) subtypes adw and ayw, including isoelectric pH evaluations, analysis of the different morphological forms, molecular weight determinations, and analysis of the polypeptides by polyacrylamide gel electrophoresis, Both HBs Ag-positive plasma and purified HBs Ag were analyzed by electrofocusing in a sucrose ampholyte gradient. Four distinct populations of HBs Ag with a pH range of 4.5 plus or minus 0.1 to 5.4 plus or minus 0.1 for unfractionated plasma samples and 3.9 plus or minus 0.05 to 4.9 plus or minus 0.05 for purified samples were detected in both adw and ayw preparations. Electron microscopic studies of each population of purified HBs Ag revealed 19- to 27-nm spheres in each fraction. Purified material labeled with 125I by the chloramine-T method behaved as one major population with an isoelectric pH value of 3.9 plus or minus 0.1. Purified adw preparations revealed a major population with a molecular weight of 3.7 times 10-6 and a second one of 4.6 times 10-6. Purified preparations of ayw contained one population with a molecular weight of 4.6 times 10-6. Polyacrylamide gel electrophoretic analysis of purified HBs Ag revealed nine polypeptides for ayw and seven for adw particles. These studies indicate that purified preparations of HBs Ag are heterogeneous and that distinct differences can be detected between the two subtypes.

Full text

PDF
182

Images in this article

186Image
on p.186
188Image
on p.188

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burrell C. J., Proudfoot E., Keen G. A., Marmion B. P. Carbohydrates in hepatitis B antigen. Nat New Biol. 1973 Jun 27;243(130):260–262. doi: 10.1038/newbio243260a0. [DOI] [PubMed] [Google Scholar]
  2. Chairez R., Hollinger F. B., Melnick J. L., Dreesman G. R. Biophysical properties of purified morphologic forms of hepatitis B antigen. Intervirology. 1974;3(3):129–140. doi: 10.1159/000149749. [DOI] [PubMed] [Google Scholar]
  3. Chairez R., Steiner S., Melnick J. L., Dreesman G. R. Glycoproteins associated with hepatitis B antigen. Intervirology. 1973;1(3):224–228. doi: 10.1159/000148850. [DOI] [PubMed] [Google Scholar]
  4. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  5. Dreesman G. R., Hollinger F. B., McCombs R. M., Melnick J. L. Production of potent anti-Australia antigen sera of high specificity and sensitivity in goats. Infect Immun. 1972 Feb;5(2):213–221. doi: 10.1128/iai.5.2.213-221.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dreesman G. R., Hollinger F. B., Suriano J. R., Fujioka R. S., Brunschwig J. P., Melnick J. L. Biophysical and biochemical heterogeneity of purified hepatitis B antigen. J Virol. 1972 Sep;10(3):469–476. doi: 10.1128/jvi.10.3.469-476.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GREENWOOD F. C., HUNTER W. M., GLOVER J. S. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963 Oct;89:114–123. doi: 10.1042/bj0890114. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gerin J. L., Holland P. V., Purcell R. H. Australia antigen: large-scale purification from human serum and biochemical studies of its proteins. J Virol. 1971 May;7(5):569–576. doi: 10.1128/jvi.7.5.569-576.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hollinger F. B., Vorndam V., Dreesman G. R. Assay of Australia antigen and antibody employing double-antibody and solid-phase radioimmunoassay techniques and comparison with the passive hemagglutination methods. J Immunol. 1971 Oct;107(4):1099–1111. [PubMed] [Google Scholar]
  10. Hollinger F. B., Wasi C., Dreesman G. R., Melnick J. L. Subtyping of hepatitis B antigen by use of monospecific antibody-coated cells. J Infect Dis. 1973 Dec;128(6):753–760. doi: 10.1093/infdis/128.6.753. [DOI] [PubMed] [Google Scholar]
  11. Howard C. R., Zuckerman A. J. Electrofocusing of hepatitis B antigen. J Gen Virol. 1973 Aug;20(2):253–256. doi: 10.1099/0022-1317-20-2-253. [DOI] [PubMed] [Google Scholar]
  12. Kim C. Y., Bissell D. M. Stability of the lipid and protein of hepatitis-associated (Australia) antigen. J Infect Dis. 1971 May;123(5):470–476. doi: 10.1093/infdis/123.5.470. [DOI] [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. Le Bouvier G. L., McCollum R. W. Australia (hepatitis-associated) antigen: physicochemical and immunological characteristics. Adv Virus Res. 1970;16:357–396. doi: 10.1016/S0065-3527(08)60027-5. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McCombs R. M., Melnick M. B., Brunschwig J. P. Biophysical studies of vesicular stomatitis virus. J Bacteriol. 1966 Feb;91(2):803–812. doi: 10.1128/jb.91.2.803-812.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Millman I., Loeb L. A., Bayer M. E., Blumberg B. S. Australia antigen (a hepatitis-associated antigen): purification and physical properties. J Exp Med. 1970 Jun 1;131(6):1190–1199. doi: 10.1084/jem.131.6.1190. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Steiner S., Huebner M. T., Dreesman G. R. Major polar lipids of hepatitis B antigen preparations: evidence for the presence of a glycosphingolipid. J Virol. 1974 Sep;14(3):572–577. doi: 10.1128/jvi.14.3.572-577.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. YPHANTIS D. A. EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964 Mar;3:297–317. doi: 10.1021/bi00891a003. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES