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. 1975 Feb;15(2):232–237. doi: 10.1128/jvi.15.2.232-237.1975

Canavanine-mediated depletion of polyamine pools in Escherichia coli: effect of head morphogenesis and DNA synthesis.

R W Bolin, D J Cummings
PMCID: PMC354444  PMID: 1089803

Abstract

We have found that L-canavanine inhibited the synthesis of polyamines in T4-infected Escherichia coli. These polyamines are known to be required for T4 DNA synthesis and may be involved in phage morphogenesis. The new data indicate that the inhibition of polyamine synthesis is not primarily responsible for the L-conavanine-mediated inhibition of DNA synthesis nor does it seem to be involved in the induction of lollipops. L-Canavanine does influence the relative amounts of putrescine and spermidine found in the phage particle, but it does not influence the amount of DNA phosphate neutralized by polyamines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
  2. BRENNER S., BARNETT L. Genetic and chemical studies on the head protein of bacteriophages T2 and T4. Brookhaven Symp Biol. 1959 Nov;12:86–94. [PubMed] [Google Scholar]
  3. Bolin R. W., Cummings D. J. Structural aberrations in T-even bacteriophage. IV. Parameters of induction and formation of lollipops. J Virol. 1974 Jun;13(6):1368–1377. doi: 10.1128/jvi.13.6.1368-1377.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bolin R. W., Cummings D. J. Structural aberrations in T-even bacteriophage. V. Effects of canavanine on the maturation and utilization of specific gene products. J Virol. 1974 Jun;13(6):1378–1391. doi: 10.1128/jvi.13.6.1378-1391.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Couse N. L., Cummings D. J., Chapman V. A., DeLong S. S. Structural aberrations in T-even bacteriophage. I. Specificity of induction of aberrations. Virology. 1970 Nov;42(3):590–602. doi: 10.1016/0042-6822(70)90305-3. [DOI] [PubMed] [Google Scholar]
  6. Couse N. L., Haworth P., Moody W., Cummings D. J. Intracellular events in canavanine-treated, T4-infected Escherichia coli. Virology. 1972 Dec;50(3):765–771. doi: 10.1016/0042-6822(72)90430-8. [DOI] [PubMed] [Google Scholar]
  7. Cummings D. J., Chapman V. A., DeLong S. S., Couse N. L. Structural aberrations in T-even bacteriophage. 3. Induction of "lollipops" and their partial characterization. Virology. 1973 Jul;54(1):245–261. doi: 10.1016/0042-6822(73)90134-7. [DOI] [PubMed] [Google Scholar]
  8. Dion A. S., Cohen S. S. Polyamines in the synthesis of bacteriophage deoxyribonucleic acid. I. Lack of dependence of polyamine synthesis on bacteriophage deoxyribonucleic acid synthesis. J Virol. 1972 Mar;9(3):419–422. doi: 10.1128/jvi.9.3.419-422.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dion A. S., Cohen S. S. Polyamines in the synthesis of bacteriophage deoxyribonucleic acid. II. Requirement for polyamines in T4 infection of a polyamine auxotroph. J Virol. 1972 Mar;9(3):423–430. doi: 10.1128/jvi.9.3.423-430.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Laemmli U. K., Favre M. Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol. 1973 Nov 15;80(4):575–599. doi: 10.1016/0022-2836(73)90198-8. [DOI] [PubMed] [Google Scholar]
  11. Luftig R. B., Wood W. B., Okinaka R. Bacteriophage T4 head morphogenesis. On the nature of gene 49-defective heads and their role as intermediates. J Mol Biol. 1971 May 14;57(3):555–573. doi: 10.1016/0022-2836(71)90109-4. [DOI] [PubMed] [Google Scholar]
  12. Morris D. R., Koffron K. L. Putrescine biosynthesis in Escherichia coli. Regulation through pathway selection. J Biol Chem. 1969 Nov 25;244(22):6094–6099. [PubMed] [Google Scholar]
  13. Morris D. R., Pardee A. B. A biosynthetic ornithine decarboxylase in Escherichia coli. Biochem Biophys Res Commun. 1965 Sep 22;20(6):697–702. doi: 10.1016/0006-291x(65)90072-0. [DOI] [PubMed] [Google Scholar]
  14. Morris D. R., Pardee A. B. Multiple pathways of putrescine biosynthesis in Escherichia coli. J Biol Chem. 1966 Jul 10;241(13):3129–3135. [PubMed] [Google Scholar]
  15. Simon L. D. Infection of Escherichia coli by T2 and T4 bacteriophages as seen in the electron microscope: T4 head morphogenesis. Proc Natl Acad Sci U S A. 1972 Apr;69(4):907–911. doi: 10.1073/pnas.69.4.907. [DOI] [PMC free article] [PubMed] [Google Scholar]

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