Abstract
Bacteriophage T4-induced modification of Escherichia coli vlayl-tRNA synthetase (EC 6.1.1.9) requires: synthesis of a phage-gene specified tau factor, addition of the factor to host valyl-tRNA synthetase to produce a urea-stable enzyme, and interaction of the modified enzyme with tRNA to produce a more rapidly sedimenting valyl-tRNA synthetase activity on sucrose density gradients. This report demonstrates that the coincident, chloramphenicol-sensitive appearance of urea-stable and rapidly sedimenting valyl-tRNA synthetase activity are immediate early phage functions. It implies that once the tau factor is synthesized, further interactions are stoichiometric rather than catalytic. The potential for valyl-tRNA synthetase modification accumylates when E. coli is infected with T4 PHAGE IN THE PRESENCE OF CHLORAMPHINICOL AND IS EXPRESSED DURING THE RESUMPTION OF PROTEIN SYNTHESIS WHEREAS FURTHER RNA synthesis is inhibited by rifampicin. The modification phenomenon occurs similarly in several strains of E. coli and represents a novel virus-host interaction.
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Selected References
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- Chrispeels M. J., Boyd R. F., Williams L. S., Neidhardt F. C. Modification of valyl tRNA synthetase by bacteriophage in Escherichia coli. J Mol Biol. 1968 Feb 14;31(3):463–475. doi: 10.1016/0022-2836(68)90421-x. [DOI] [PubMed] [Google Scholar]
- EIDLIC L., NEIDHARDT F. C. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES. J Bacteriol. 1965 Mar;89:706–711. doi: 10.1128/jb.89.3.706-711.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Lembach K. J., Buchanan J. M. The relationship of protein synthesis to early transcriptive events in bacteriophage T4-infected Escherichia coli B. J Biol Chem. 1970 Apr 10;245(7):1575–1587. [PubMed] [Google Scholar]
- Lembach K. J., Kuninaka A., Buchanan J. M. The relationship of DNA replication to the control of protein synthesis in protoplasts of T4-infected Escherichia coli B. Proc Natl Acad Sci U S A. 1969 Feb;62(2):446–453. doi: 10.1073/pnas.62.2.446. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marchin G. L., Comer M. M., Neidhardt F. C. Viral modification of the valyl transfer ribonucleic acid synthetase of Escherichia coli. J Biol Chem. 1972 Aug 25;247(16):5132–5145. [PubMed] [Google Scholar]
- Marchin G. L., Müller U. R., al-Khateeb G. H. The effect of transfer ribonucleic acid on virally modified valyl transfer ribonucleic acid synthetase of Escherichia coli. J Biol Chem. 1974 Aug 10;249(15):4705–4711. [PubMed] [Google Scholar]
- Neidhardt F. C., Marchin G. L., McClain W. H., Boyd R. F., Earhart C. F. Phage-induced modification of valyl-tRNA synthetase. J Cell Physiol. 1969 Oct;74(2 Suppl):87+–87+. doi: 10.1002/jcp.1040740408. [DOI] [PubMed] [Google Scholar]
- Warner H. R., Lewis N. The synthesis of deoxycytidylate deaminase and dihydrofolate reductase and its control in Escherichia coli infected with bacteriophage T4 and T-4 amber mutants. Virology. 1966 May;29(1):172–175. doi: 10.1016/0042-6822(66)90208-x. [DOI] [PubMed] [Google Scholar]