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. 1975 Feb;15(2):363–371. doi: 10.1128/jvi.15.2.363-371.1975

Inhibition of bacteriophage PBS2 replication in Bacillus subtilis by phleomycin.

L Post, A R Price
PMCID: PMC354461  PMID: 163361

Abstract

Phleomycin is an effective inhibitor of the replication of Bacillus subtilis bacteriophage PBS2, whose DNA contains uracil instead of thymine. Phleomycin does not affect the induction of the known phage enzymes involved in deoxyribonucleotide metabolism. But phage DNA synthesis is severely inhibited by phleomycin, and late virion protein synthesis is eliminated. These effects appear to result from a phleomycin-induced degradation of the parental phage DNA. Similar inhibitory and degradative effects on DNA are seen in phleomyinc-treated, uninfected cells. This system is unaffected by the related antibiotic, bleomycin.

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Selected References

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  1. Ames G. F. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions. J Biol Chem. 1974 Jan 25;249(2):634–644. [PubMed] [Google Scholar]
  2. Bauernfeind A. Mode of action of nalidixic acid. Antibiot Chemother (1971) 1971;17:122–136. doi: 10.1159/000392368. [DOI] [PubMed] [Google Scholar]
  3. Bazill G. W., Karamata D. Temperature-sensitive mutants of B. subtilis defective in deoxyribonucleotide synthesis. Mol Gen Genet. 1972;117(1):19–29. [PubMed] [Google Scholar]
  4. FALASCHI A., KORNBERG A. ANTIMETABOLITES AFFECTING PROTEIN OR NUCLEIC ACID SYNTHESIS. PHLEOMYCIN, AN INHIBITOR OF DNA POLYMERASE. Fed Proc. 1964 Sep-Oct;23:940–945. [PubMed] [Google Scholar]
  5. Farrell L., Reiter H. Phleomycin-stimulated degradation of deoxyribonucleic acid in Escherichia coli. Antimicrob Agents Chemother. 1973 Sep;4(3):320–326. doi: 10.1128/aac.4.3.320. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gass K. B., Low R. L., Cozzarelli N. R. Inhibition of a DNA polymerase from Bacillus subtilis by hydroxyphenylazopyrimidines. Proc Natl Acad Sci U S A. 1973 Jan;70(1):103–107. doi: 10.1073/pnas.70.1.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Grigg G. W., Edwards M. J., Brown D. J. Effects of coumarin, thiopurines, and pyronin Y on amplification of phleomycin-induced death and deoxyribonucleic acid breakdown in Escherichia coli. J Bacteriol. 1971 Sep;107(3):599–609. doi: 10.1128/jb.107.3.599-609.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Grigg G. W. Induction of DNA breakdown and death in Escherichia coli by phleomycin. Its association with dark-repair processes. Mol Gen Genet. 1969;104(1):1–11. doi: 10.1007/BF00277357. [DOI] [PubMed] [Google Scholar]
  9. IKEKAWA T., IWAMI F., HIRANAKA H., UMEZAWA H. SEPARATION OF PHLEOMYCIN COMPONENTS AND THEIR PROPERTIES. J Antibiot (Tokyo) 1964 Sep;17:194–199. [PubMed] [Google Scholar]
  10. Ishizuka M., Takayama H., Takeuchi T., Umezawa H. Studies on antitumor activity, antimicrobial activity and toxicity of phleomycin. J Antibiot (Tokyo) 1966 Nov;19(6):260–271. [PubMed] [Google Scholar]
  11. Koch G. Differential effect of phleomycin on the infectivity of poliovirus and poliovirus-induced ribonucleic acids. J Virol. 1971 Jul;8(1):28–34. doi: 10.1128/jvi.8.1.28-34.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Krueger W. C., Pschigoda L. M., Reusser F. Interactions of DNA with zorbamycin, phleomycin and bleomycin; ultraviolet absorption and circular dichroism measurements. J Antibiot (Tokyo) 1973 Aug;26(8):424–428. doi: 10.7164/antibiotics.26.424. [DOI] [PubMed] [Google Scholar]
  13. Pietsch P. Biopolymers in drug technology: the use of DNA in purifying phleomycin. Biotechnol Bioeng. 1973 Nov;15(6):1039–1044. doi: 10.1002/bit.260150604. [DOI] [PubMed] [Google Scholar]
  14. Pietsch P., Garrett H. Primary site of reaction in the in vitro complex of phleomycin in DNA. Nature. 1968 Aug 3;219(5153):488–489. doi: 10.1038/219488a0. [DOI] [PubMed] [Google Scholar]
  15. Pitts J. D., Sinsheimer R. L. Effect of phleomycin upon replication of bacteriophage phiX174. J Mol Biol. 1966 Feb;15(2):676–680. doi: 10.1016/s0022-2836(66)80136-5. [DOI] [PubMed] [Google Scholar]
  16. Price A. R. Bacteriophage PBS2-induced deoxycytidine triphosphate deaminase in Bacillus subtilis. J Virol. 1974 Nov;14(5):1314–1317. doi: 10.1128/jvi.14.5.1314-1317.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Price A. R., Cook S. J. New deoxyribonucleic acid polymerase induced by Bacillus subtilis bacteriophage PBS2. J Virol. 1972 Apr;9(4):602–610. doi: 10.1128/jvi.9.4.602-610.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Price A. R., Fogt S. M. Deoxythymidylate phosphohydrolase induced by bacteriophage PBS2 during infection of Bacillus subtilis. J Biol Chem. 1973 Feb 25;248(4):1372–1380. [PubMed] [Google Scholar]
  19. Price A. R., Fogt S. M. Effect of nalidixic acid on PBS2 bacteriophage infection of Bacillus subtilis. J Virol. 1973 Aug;12(2):405–407. doi: 10.1128/jvi.12.2.405-407.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Price A. R., Fogt S. M. Resistance of bacteriophage PBS2 infection to 6-(p-hydroxyphenylazo)-uracil, an inhibitor of Bacillus subtilis deoxyribonucleic acid synthesis. J Virol. 1973 Feb;11(2):338–340. doi: 10.1128/jvi.11.2.338-340.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Price A. R., Frabotta M. Resistance of bacteriophage PBS2 infection to rifampicin, an inhibitor of Bacillus subtilis RNA synthesis. Biochem Biophys Res Commun. 1972 Sep 26;48(6):1578–1585. doi: 10.1016/0006-291x(72)90894-7. [DOI] [PubMed] [Google Scholar]
  22. Reiter H., Milewskiy M., Kelley P. Mode of action of phleomycin on Bacillus subtilis. J Bacteriol. 1972 Aug;111(2):586–592. doi: 10.1128/jb.111.2.586-592.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rima B. K., Takahashi I. The synthesis of nucleic acids in Bacillus subtilis infected with phage PBS 1. Can J Biochem. 1973 Sep;51(9):1219–1224. doi: 10.1139/o73-161. [DOI] [PubMed] [Google Scholar]
  24. Saunders P. P., Schultz G. A. Mechanism of action of bleomycin. I. Bacterial growth studies. Biochem Pharmacol. 1972 Jun 15;21(12):1657–1666. doi: 10.1016/0006-2952(72)90073-1. [DOI] [PubMed] [Google Scholar]
  25. TAKITA T. Studies on purification and properties of phleomycin. J Antibiot (Tokyo) 1959 Nov;12:285–289. [PubMed] [Google Scholar]
  26. Takita T., Muraoka Y., Yoshioka T., Fujii A., Maeda K. The chemistry of bleomycin. IX. The structures of bleomycin and phleomycin. J Antibiot (Tokyo) 1972 Dec;25(12):755–757. doi: 10.7164/antibiotics.25.755. [DOI] [PubMed] [Google Scholar]
  27. Warner H. R., Barnes J. E. Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4. Virology. 1966 Jan;28(1):100–107. doi: 10.1016/0042-6822(66)90310-2. [DOI] [PubMed] [Google Scholar]
  28. Watanabe M., August J. T. Replication of RNA bacteriophage R23. II. Inhibition of phage-specific RNA synthesis by phleomycin. J Mol Biol. 1968 Apr 14;33(1):21–33. doi: 10.1016/0022-2836(68)90278-7. [DOI] [PubMed] [Google Scholar]
  29. Watson R., Yamazaki H. Alkali hydrolysis of RNA of Escherichia coli deposited on filter paper disks. Anal Biochem. 1973 Jan;51(1):312–314. doi: 10.1016/0003-2697(73)90479-x. [DOI] [PubMed] [Google Scholar]

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