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. 1975 Mar;15(3):439–448. doi: 10.1128/jvi.15.3.439-448.1975

Proteins in intracellular simian virus 40 nucleoportein complexes: comparison with simian virus 40 core proteins.

W Meinke, M R Hall, D A Goldstein
PMCID: PMC354478  PMID: 163367

Abstract

Intracellular nucleoprotein complexes containing SV40 supercoiled DNA were purified from cell lysates by chromatography on hydroxyapatite columns followed by velocity sedimentation through sucrose gradients. The major protein components from purified complexes were identified as histone-like proteins. When analyzed by electrophoresis in sodium dodecyl sulfate-polyacrylamide gels, complex proteins comigrated with viral core polypeptides VP4, VP5, VP6, and VP7. (3H) tryptophan was not detected in polypeptides from intracellular complexes or in the histone components from purified SV40 virus. However, a large amount of (3H) tryptophan was found in the viral polypeptide VP3 relative to that incorporated into the capsid polypeptides VP1 and VP2. Intracellular complexes contain 30 to 40% more protein than viral cores prepared by alkali dissociation of intact virus, but when complexes were exposed to the same alkaline conditions, protein also was removed from complexes and they subsequently co-sedimented with and had the same buoyant density as viral cores. The composition and physical similarities of nucleoprotein complex and viral cores indicate that complexes may have a role in the assembly of virions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderer F. A., Schlumberger H. D., Koch M. A., Frank H., Eggers H. J. Structure of simian virus 40. II. Symmetry and components of the virus particle. Virology. 1967 Jul;32(3):511–523. doi: 10.1016/0042-6822(67)90303-0. [DOI] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S. Isopycnic separation of subcellular components from poliovirus-infected and normal HeLa cells. Science. 1968 Nov 1;162(3853):572–574. doi: 10.1126/science.162.3853.572. [DOI] [PubMed] [Google Scholar]
  3. Barban S., Goor R. S. Structural proteins of simian virus 40. J Virol. 1971 Feb;7(2):198–203. doi: 10.1128/jvi.7.2.198-203.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Estes M. K., Huang E. S., Pagano J. S. Structural polypeptides of simian virus 40. J Virol. 1971 May;7(5):635–641. doi: 10.1128/jvi.7.5.635-641.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Friedmann T. Genetic economy of polyoma virus: capsid proteins are cleavage products of same viral gene. Proc Natl Acad Sci U S A. 1974 Feb;71(2):257–259. doi: 10.1073/pnas.71.2.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Friedmann T. In vitro reassembly of shell-like particles from disrupted polyoma virus. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2574–2578. doi: 10.1073/pnas.68.10.2574. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. GIRARDI A. J. The use of fluorocarbon for "unmasking" polyoma virus hemagglutinin. Virology. 1959 Nov;9:488–489. doi: 10.1016/0042-6822(59)90141-2. [DOI] [PubMed] [Google Scholar]
  8. Girard M., Marty L., Suarez F. Capsid proteins of Simian virus 40. Biochem Biophys Res Commun. 1970 Jul 13;40(1):97–102. doi: 10.1016/0006-291x(70)91051-x. [DOI] [PubMed] [Google Scholar]
  9. Goldstein D. A., Hall M. R., Meinke W. Properties of nucleoprotein complexes containing replicating polyoma DNA. J Virol. 1973 Oct;12(4):887–900. doi: 10.1128/jvi.12.4.887-900.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Green M. H. Biosynthetic properties of a polyoma nucleoprotein complex: evidence for replication sites. J Virol. 1972 Jul;10(1):32–41. doi: 10.1128/jvi.10.1.32-41.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green M. H., Miller H. I., Hendler S. Isolation of a polyoma-nucleoprotein complex from infected mouse-cell cultures. Proc Natl Acad Sci U S A. 1971 May;68(5):1032–1036. doi: 10.1073/pnas.68.5.1032. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hall M. R., Meinke W., Goldstein D. A. Nucleoprotein complexes containing replicating Simian virus 40 DNA: comparison with polyoma nucleoprotein complexes. J Virol. 1973 Oct;12(4):901–908. doi: 10.1128/jvi.12.4.901-908.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Huang E. S., Estes M. K., Pagano J. S. Structure and function of the polypeptides in simian virus 40. I. Existence of subviral deoxynucleoprotein complexes. J Virol. 1972 Jun;9(6):923–929. doi: 10.1128/jvi.9.6.923-929.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lake R. S., Barban S., Salzman N. P. Resolutions and identification of the core deoxynucleoproteins of the simian virus 40. Biochem Biophys Res Commun. 1973 Sep 18;54(2):640–647. doi: 10.1016/0006-291x(73)91471-x. [DOI] [PubMed] [Google Scholar]
  15. Meinke W., Goldstein D. A. Studies on the structure and formation of polyoma DNA replicative intermediates. J Mol Biol. 1971 Nov 14;61(3):543–563. doi: 10.1016/0022-2836(71)90064-7. [DOI] [PubMed] [Google Scholar]
  16. Ozer H. L. Synthesis and assembly of simian virus 40. I. Differential synthesis of intact virions and empty shells. J Virol. 1972 Jan;9(1):41–51. doi: 10.1128/jvi.9.1.41-51.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. PERLMANN G. E. The nature of phosphorus linkages in phosphoproteins. Adv Protein Chem. 1955;10:1–30. doi: 10.1016/s0065-3233(08)60103-5. [DOI] [PubMed] [Google Scholar]
  18. Pardon J. F., Wilkins M. H. A super-coil model for nucleohistone. J Mol Biol. 1972 Jul 14;68(1):115–124. doi: 10.1016/0022-2836(72)90267-7. [DOI] [PubMed] [Google Scholar]
  19. Robb J. A., Martin R. G. Genetic analysis of simian virus 40. 3. Characterization of a temperature-sensitive mutant blocked at an early stage of productive infection in monkey cells. J Virol. 1972 Jun;9(6):956–968. doi: 10.1128/jvi.9.6.956-968.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Seebeck T., Weil R. Polyoma viral DNA replicated as a nucleoprotein complex in close association with the host cell chromatin. J Virol. 1974 Mar;13(3):567–576. doi: 10.1128/jvi.13.3.567-576.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sen A., Levine A. J. SV40 nucleoprotein complex activity unwinds superhelical turns in SV40 DNA. Nature. 1974 May 24;249(455):343–344. doi: 10.1038/249343a0. [DOI] [PubMed] [Google Scholar]
  22. Shmookler R. J., Buss J., Green M. H. Properties of the polyoma virus transcription complex obtained from mouse nuclei. Virology. 1974 Jan;57(1):122–127. doi: 10.1016/0042-6822(74)90113-5. [DOI] [PubMed] [Google Scholar]
  23. Tan K. B., Sokol F. Structural proteins of simian virus 40: phosphoproteins. J Virol. 1972 Nov;10(5):985–994. doi: 10.1128/jvi.10.5.985-994.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  25. Walter G., Roblin R., Dulbecco R. Protein synthesis in Simian virus 40-infected monkey cells. Proc Natl Acad Sci U S A. 1972 Apr;69(4):921–924. doi: 10.1073/pnas.69.4.921. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. White M., Eason R. Nucleoprotein complexes in simian virus 40-infected cells. J Virol. 1971 Oct;8(4):363–371. doi: 10.1128/jvi.8.4.363-371.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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