Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Mar;15(3):449–457. doi: 10.1128/jvi.15.3.449-457.1975

RNA of simian sarcoma-associated virus type 1 produced in human tumor cells.

H M Fidanián, W N Drohán, M A Baluda
PMCID: PMC354479  PMID: 46285

Abstract

Simian sarcoma-associated virus type 1 propagated in human rhabdomyosarcoma cells exhibited characteristics typical of oncornaviruses but seemed to have several aberrant properties. It had a buoyant density of 1.14 g/cm3, had RNA-dependent DNA polymerase activity, seemed to be labile to high salt concentrations, and contained little 50 to 60S RNA but relatively large amounts of human ribosomal RNA. In addition to 50 to 60S RNA, purified virions contained smaller RNA molecules with sedimentation coefficients of 28 to 30S, 18 TO 20S, and 4 to 10S. Unlike the 50 to 60S RNA species, the smaller virion-associated RNAs lacked polyadenylic acid, and the 28 to 30S RNA had an average base composition similar to that of human ribosomal RNA. Upon heat denaturation, the native 50 to 60S RNA genome yielded polyadenylic acid-containing 28 to 30S subunits that degraded in to 18 to 20S molecules upon further heat treatment. The 50 to 60S viral RNA had a guanine plus cytosine content of 56%.

Full text

PDF
449

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BALUDA M. A., GOETZ I. E. Morphological conversion of cell cultures by avian myeloblastosis virus. Virology. 1961 Oct;15:185–199. doi: 10.1016/0042-6822(61)90234-3. [DOI] [PubMed] [Google Scholar]
  2. Baluda M. A., Markham P. D. Nucleotide composition of RNA hybridized to homologous DNA from cells transformed by avian tumour viruses. Nat New Biol. 1971 May 19;231(20):90–91. doi: 10.1038/newbio231090a0. [DOI] [PubMed] [Google Scholar]
  3. Baluda M. A., Nayak D. P. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969 Nov;4(5):554–566. doi: 10.1128/jvi.4.5.554-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benveniste R. E., Todaro G. J. Homology between type-C viruses of various species as determined by molecular hybridization. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3316–3320. doi: 10.1073/pnas.70.12.3316. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bolognesi D. P., Obara T. Minor RNA and other components of host origin intrinsic to avian leukosis virus particles. Bibl Haematol. 1970;(36):126–139. doi: 10.1159/000391700. [DOI] [PubMed] [Google Scholar]
  7. Bonar R. A., Sverak L., Bolognesi D. P., Langlois A. J., Beard D., Beard J. W. Ribonucleic acid components of BAI strain A (myeloblastosis) avian tumor virus. Cancer Res. 1967 Jun;27(6):1138–1157. [PubMed] [Google Scholar]
  8. East J. L., Allen P. T., Knesek J. E., Chan J. C., Bowen J. M., Dmochowski L. Structural rearrangement and subunit composition of RNA from released Soehner-Dmochowski murine sarcoma virions. J Virol. 1973 May;11(5):709–720. doi: 10.1128/jvi.11.5.709-720.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Faras A. J., Garapin A. C., Levinson W. E., Bishop J. M., Goodman H. M. Characterization of the low-molecular-weight RNAs associated with the 70S RNA of Rous sarcoma virus. J Virol. 1973 Aug;12(2):334–342. doi: 10.1128/jvi.12.2.334-342.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gallagher R. E., Todaro G. J., Smith R. G., Livingston D. M., Gallo R. C. Relationship between RNA-directed DNA polymerase (reverse transcriptase) from human acute leukemic blood cells and primate type-C viruses. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1309–1313. doi: 10.1073/pnas.71.4.1309. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gay F. W., Clarke J. K., Dermott E. Morphogenesis of Bittner virus. J Virol. 1970 Jun;5(6):801–806. doi: 10.1128/jvi.5.6.801-816.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Green M., Cartas M. The genome of RNA tumor viruses contains polyadenylic acid sequences. Proc Natl Acad Sci U S A. 1972 Apr;69(4):791–794. doi: 10.1073/pnas.69.4.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Harewood K. R., Vidrine J. G., Larson D. L., Wolff J. S., 3rd, Schidlovsky G., Mayyasi S. A. Biochemical and morphological studies of Simian Sarcoma Virus, Type 1. Biochim Biophys Acta. 1973 May 10;308(2):252–259. doi: 10.1016/0005-2787(73)90155-x. [DOI] [PubMed] [Google Scholar]
  14. Holder W. D., Jr, Robey W. G., Vande Woude G. F. Activation of a C-type virus from the human carcinoma cell line HBT-3 by iododeoxyuridine and testosterone. Nature. 1974 Jun 21;249(459):759–762. doi: 10.1038/249759a0. [DOI] [PubMed] [Google Scholar]
  15. Ikawa Y., Ross J., Leder P. An association between globin messenger RNA and 60S RNA derived from Friend leukemia virus. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1154–1158. doi: 10.1073/pnas.71.4.1154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Jensik S., Hoekstra J., Silver S., Northrop R. L., Deinhardt F. The 60 to 70S RNA and reverse transcriptase of simian sarcoma and simian sarcoma-associated viruses. Intervirology. 1973;1(4):229–241. doi: 10.1159/000148851. [DOI] [PubMed] [Google Scholar]
  17. Lai M. M., Duesberg P. H. Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature. 1972 Feb 18;235(5338):383–386. doi: 10.1038/235383c0. [DOI] [PubMed] [Google Scholar]
  18. Levin J. G., Grimley P. M., Ramseur J. M., Berezesky I. K. Deficiency of 60 to 70S RNA in murine leukemia virus particles assembled in cells treated with actinomycin D. J Virol. 1974 Jul;14(1):152–161. doi: 10.1128/jvi.14.1.152-161.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Levinson W., Bishop J. M., Quintrell N., Jackson J. Presence of DNA in Rous sarcoma virus. Nature. 1970 Sep 5;227(5262):1023–1025. doi: 10.1038/2271023a0. [DOI] [PubMed] [Google Scholar]
  20. Manning J. S., Schaffer F. L., Soergel M. E. Correlation between murine sarcoma virus buoyant density, infectivity, and viral RNA electrophoretic mobility. Virology. 1972 Sep;49(3):804–807. doi: 10.1016/0042-6822(72)90537-5. [DOI] [PubMed] [Google Scholar]
  21. Obara T., Bolognesi D. P., Bauer H. Ribosomal RNA in avian leukosis virus particles. Int J Cancer. 1971 May 15;7(3):535–546. doi: 10.1002/ijc.2910070320. [DOI] [PubMed] [Google Scholar]
  22. Phillips L. A., Hollis V. W., Jr, Bassin R. H., Fischinger P. J. Characterization of RNA from noninfectious virions produced by sarcoma positive-leukemia negative transformed 3T3 cells. Proc Natl Acad Sci U S A. 1973 Oct;70(10):3002–3006. doi: 10.1073/pnas.70.10.3002. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ross J., Tronick S. R., Scolnick E. M. Polyadenylate rich RNA in the 70 S RNA of murine leukemia-sarcoma virus. Virology. 1972 Jul;49(1):230–235. doi: 10.1016/s0042-6822(72)80025-4. [DOI] [PubMed] [Google Scholar]
  24. Sheldon R., Jurale C., Kates J. Detection of polyadenylic acid sequences in viral and eukaryotic RNA(polu(U)-cellulose columns-poly(U) filters-fiberglass-HeLa cells-bacteriophage T4). Proc Natl Acad Sci U S A. 1972 Feb;69(2):417–421. doi: 10.1073/pnas.69.2.417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sherr C. J., Lieber M. M., Benveniste R. E., Todaro G. J. Endogenous baboon type C virus (M7): biochemical and immunologic characterization. Virology. 1974 Apr;58(2):492–503. doi: 10.1016/0042-6822(74)90083-x. [DOI] [PubMed] [Google Scholar]
  26. Smetana K., Korb J., Ríman J. On the presence of ribosome-like particles in avian myeloblastosis virions (AMV) and unusual ultrastructure of their nucleoid. Neoplasma. 1972;19(5):437–441. [PubMed] [Google Scholar]
  27. Soeiro R., Birnboim H. C., Darnell J. E. Rapidly labeled HeLa cell nuclear RNA. II. Base composition and cellular localization of a heterogeneous RNA fraction. J Mol Biol. 1966 Aug;19(2):362–372. doi: 10.1016/s0022-2836(66)80010-4. [DOI] [PubMed] [Google Scholar]
  28. Stephenson M. L., Wirthlin L. S., Scott J. F., Zamecnik P. C. The 3'-terminal nucleosides of the high molecular weight RNA of avian myeloblastosis virus. Proc Natl Acad Sci U S A. 1972 May;69(5):1176–1180. doi: 10.1073/pnas.69.5.1176. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Syrewicz J. J., Naso R. B., Wang C. S., Arlinghaus R. B. Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures. Appl Microbiol. 1972 Sep;24(3):488–494. doi: 10.1128/am.24.3.488-494.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Theilen G. H., Gould D., Fowler M., Dungworth D. L. C-type virus in tumor tissue of a woolly monkey (Lagothrix spp.) with fibrosarcoma. J Natl Cancer Inst. 1971 Oct;47(4):881–889. [PubMed] [Google Scholar]
  31. Varmus H. E., Levinson W. E., Bishop J. M. Extent of transcription by the RNA-dependent DNA polymerase of Rous sarcoma virus. Nat New Biol. 1971 Sep 1;233(35):19–21. doi: 10.1038/newbio233019a0. [DOI] [PubMed] [Google Scholar]
  32. Wagner A. F., Bugianesi R. L., Shen T. Y. Preparation of sepharose-bound poly (rI:rC). Biochem Biophys Res Commun. 1971 Oct 1;45(1):184–189. doi: 10.1016/0006-291x(71)90067-2. [DOI] [PubMed] [Google Scholar]
  33. Wolfe L. G., Deinhardt F., Theilen G. H., Rabin H., Kawakami T., Bustad L. K. Induction of tumors in marmoset monkeys by simian sarcoma virus, type 1 (Lagothrix): a preliminary report. J Natl Cancer Inst. 1971 Nov;47(5):1115–1120. [PubMed] [Google Scholar]
  34. Wolfe L. G., Smith R. K., Deinhardt F. Simian sarcoma virus, type 1 (Lagothrix): focus assay and demonstration of nontransforming associated virus. J Natl Cancer Inst. 1972 Jun;48(6):1905–1908. [PubMed] [Google Scholar]
  35. Wollmann R. L., Kirsten W. H. Cellular origin of a mouse leukemia viral ribonucleic acid. J Virol. 1968 Nov;2(11):1241–1248. doi: 10.1128/jvi.2.11.1241-1248.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES