Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Mar;15(3):534–539. doi: 10.1128/jvi.15.3.534-539.1975

Induction of human cell DNA synthesis by herpes simplex virus type 2.

P Melvin, L S Kucera
PMCID: PMC354490  PMID: 163370

Abstract

The effect of herpes simplex virus type 2 (HSV-2) infection on the synthesis of DNA in human embryonic fibroblast cells was determined at temperatures permissive (37 C) and nonpermissive (42 C) for virus multiplication. During incubation of HSV-2 infected cultures at 42 C for 2 to 4 days or after shift-down from 42 to 37 C, incorporation of (3H)TdR into total DNA was increased 2-to 30-fold as compared with mock-infected cultures. Analysis of the (3H)DNA suggested that host cell DNA synthesis was induced by HSV-2 infection. Induction of host cell DNA synthesis by HSV-2 also occurred in cells arrested in DNA replication by low serum concentration. The three strains of HSV-2 tested were capable of stimulating cellular DNA synthesis. Virus inactivated by UV irradiation, heat, or neutral red dye and light did not induce cellular DNA synthesis, suggesting that an active viral genome is necessary for induction.

Full text

PDF
534

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrecht T., Rapp F. Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet-irradiated human cytomegalovirus. Virology. 1973 Sep;55(1):53–61. doi: 10.1016/s0042-6822(73)81007-4. [DOI] [PubMed] [Google Scholar]
  2. Cohen G. H., Vaughan R. K., Lawrence W. C. Deoxyribonucleic acid synthesis in synchronized mammalian KB cells infected with herpes simplex virus. J Virol. 1971 Jun;7(6):783–791. doi: 10.1128/jvi.7.6.783-791.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Crouch N. A., Rapp F. Cell-dependent differences in the production of infectious herpes simplex virus at a supraoptimal temperature. J Virol. 1972 Feb;9(2):223–230. doi: 10.1128/jvi.9.2.223-230.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Darai G., Munk K. Human embryonic lung cells abortively infected with Herpes virus hominis type 2 show some properties of cell transformation. Nat New Biol. 1973 Feb 28;241(113):268–269. doi: 10.1038/newbio241268a0. [DOI] [PubMed] [Google Scholar]
  6. Fox T. O., Levine A. J. Relationship between virus-induced cellular deoxyribonucleic acid synthesis and transformation by simian virus 40. J Virol. 1971 Apr;7(4):473–477. doi: 10.1128/jvi.7.4.473-477.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fried M., Pitts J. D. Replication of polyoma virus DNA. I. A resting cell system for biochemical studies on polyoma virus. Virology. 1968 Apr;34(4):761–770. doi: 10.1016/0042-6822(68)90097-4. [DOI] [PubMed] [Google Scholar]
  8. GUDNADOTTIR M., HELGADOTTIR H., BJARNASON O., JONSDOTTIR K. VIRUS ISOLATED FROM THE BRAIN OF A PATIENT WITH MULTIPLE SCLEROSIS. Exp Neurol. 1964 Feb;9:85–95. doi: 10.1016/0014-4886(64)90008-1. [DOI] [PubMed] [Google Scholar]
  9. Gerber P., Hoyer B. H. Induction of cellular DNA synthesis in human leukocytes by Epstein-Barr virus. Nature. 1971 May 7;231(5297):46–47. doi: 10.1038/231046a0. [DOI] [PubMed] [Google Scholar]
  10. Gershon D., Hausen P., Sachs L., Winocour E. On the mechanism of polyoma virus-induced synthesis of cellular DNA. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1584–1592. doi: 10.1073/pnas.54.6.1584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gershon D., Sachs L., Winocour E. The induction of cellular DNA synthesis by simian virus 40 in contact-inhibited and in x-irradiated cells. Proc Natl Acad Sci U S A. 1966 Sep;56(3):918–925. doi: 10.1073/pnas.56.3.918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hirai K., Robb J. A., Defendi V. Integration of SV40 DNA and induction of cellular DNA synthesis after a ts SV40 infection. Virology. 1974 May;59(1):266–274. doi: 10.1016/0042-6822(74)90222-0. [DOI] [PubMed] [Google Scholar]
  13. Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. Stimulation of cellular DNA synthesis by human cytomegalovirus. J Virol. 1974 Feb;13(2):353–362. doi: 10.1128/jvi.13.2.353-362.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rapp F. Herpesviruses and cancer. Adv Cancer Res. 1974;19(0):265–302. doi: 10.1016/s0065-230x(08)60056-1. [DOI] [PubMed] [Google Scholar]
  15. Rapp F., Li J. L., Jerkofsky M. Transformation of mammalian cells by DNA-containing viruses following photodynamic inactivation. Virology. 1973 Oct;55(2):339–346. doi: 10.1016/0042-6822(73)90173-6. [DOI] [PubMed] [Google Scholar]
  16. Schnipper L. E., Lewis A. M., Jr, Levine A. S. Induction of cellular DNA synthesis by the nondefective Adenovirus 2-Simian virus 40 hybrid viruses. J Virol. 1973 Oct;12(4):940–943. doi: 10.1128/jvi.12.4.940-943.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Shope T., Dechairo D., Miller G. Malignant lymphoma in cottontop marmosets after inoculation with Epstein-Barr virus. Proc Natl Acad Sci U S A. 1973 Sep;70(9):2487–2491. doi: 10.1073/pnas.70.9.2487. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Takahashi M., Ogino T., Baba K., Onaka M. Synthesis of deoxyribonucleic acid in human and hamster kidney cells infected with human adenovirus types 5 and 12. Virology. 1969 Apr;37(4):513–520. doi: 10.1016/0042-6822(69)90269-4. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES