Abstract
Oncornaviruses, which contain a virion-associated protein kinase, were found to possess phosphoproteins as virion structural components. One major phosphoprotein common to strains of laboratory and wild mouse oncornaviruses and a strain of feline leukemia virus was shown to be a polypeptide of about 12, 000 mol wt. In addition to this, the Kirsten strain of murine sarcoma virus contained a second major phosphoprotein of about 10, 000 mol wt, and mouse erythroblastosis virus contained a second major phosphoprotein that was either identical to or comigrated with the virion glycoprotein of about 74, 000 mol wt. The major phosphoprotein of RD-114 virus was found to be of about 16, 000 mol wt. The major phosphoamino acid of the 12, 000-mol wt polypeptide of the mouse erythroblastosis virus was identified as phosphoserine, and that of the 16, 000-mol wt polypeptide of the RD-114 virus was identified as phosphothreonine.
Full text
PDF









Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allerton S. E., Perlmann G. E. Chemical characterization of the phosphoprotein phosvitin. J Biol Chem. 1965 Oct;240(10):3892–3898. [PubMed] [Google Scholar]
- August J. T., Bolognesi D. P., Fleissner E., Gilden R. V., Nowinski R. C. A proposed nomenclature for the virion proteins of oncogenic RNA viruses. Virology. 1974 Aug;60(2):595–600. doi: 10.1016/0042-6822(74)90356-0. [DOI] [PubMed] [Google Scholar]
- Benveniste R. E., Heinemann R., Wilson G. L., Callahan R., Todaro G. J. Detection of baboon type C viral sequences in various primate tissues by molecular hybridization. J Virol. 1974 Jul;14(1):56–67. doi: 10.1128/jvi.14.1.56-67.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner M. B., Henderson B. E., Officer J. E., Rongey R. W., Parker J. C., Oliver C., Estes J. D., Huebner R. J. A spontaneous lower motor neuron disease apparently caused by indigenous type-C RNA virus in wild mice. J Natl Cancer Inst. 1973 Oct;51(4):1243–1254. doi: 10.1093/jnci/51.4.1243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gardner M. B., Officer J. E., Rongey R. W., Charman H. P., Hartley J. W., Estes J. D., Huebner R. J. C-type RNA tumor virus in wild house mice (Mus musculus). Bibl Haematol. 1973;39:335–344. doi: 10.1159/000427860. [DOI] [PubMed] [Google Scholar]
- Gardner M. B., Rasheed S., Rongey R. W., Charman H. P., Alena B., Gilden R. V., Huebner R. J. Natural expression of feline type-C virus genomes, prevalence of detectable felv and RD-114 GS antigen, type-C particles and infectious virus in postnatal and fetal cats. Int J Cancer. 1974 Jul 15;14(1):97–105. doi: 10.1002/ijc.2910140112. [DOI] [PubMed] [Google Scholar]
- Green R. W., Bolognesi D. P., Schäfer W., Pister L., Hunsmann G., De Noronha F. Polypeptides of mammalian oncornaviruses. I. Isolation and serological analysis polypeptides from murine and feline C-type viruses. Virology. 1973 Dec;56(2):565–579. doi: 10.1016/0042-6822(73)90058-5. [DOI] [PubMed] [Google Scholar]
- Hatanaka M., Twiddy E., Gilden R. V. Protein kinase associated with RNA tumor viruses and other budding RNA viruses. Virology. 1972 Feb;47(2):536–538. doi: 10.1016/0042-6822(72)90297-8. [DOI] [PubMed] [Google Scholar]
- Huu Duc-Nguyen, Rosenblum E. N., Zeigel R. F. Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. J Bacteriol. 1966 Oct;92(4):1133–1140. doi: 10.1128/jb.92.4.1133-1140.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maisel J., Klement V., Lai M. M., Ostertag W., Duesberg P. Ribonucleic acid components of murine sarcoma and leukemia viruses. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3536–3540. doi: 10.1073/pnas.70.12.3536. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Maizel J. V., Jr Acrylamide-gel electrophorograms by mechanical fractionation: radioactive adenovirus proteins. Science. 1966 Feb 25;151(3713):988–990. doi: 10.1126/science.151.3713.988. [DOI] [PubMed] [Google Scholar]
- McAllister R. M., Nicolson M., Gardner M. B., Rasheed S., Rongey R. W., Hardy W. D., Jr, Gilden R. V. RD-114 virus compared with feline and murine type-C viruses released from RD cells. Nat New Biol. 1973 Mar 21;242(116):75–78. doi: 10.1038/newbio242075a0. [DOI] [PubMed] [Google Scholar]
- McAllister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V. C-type virus released from cultured human rhabdomyosarcoma cells. Nat New Biol. 1972 Jan 5;235(53):3–6. doi: 10.1038/newbio235003a0. [DOI] [PubMed] [Google Scholar]
- Nowinski R. C., Fleissner E., Sarkar N. H., Aoki T. Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. II. Mammalian leukemia-sarcoma viruses. J Virol. 1972 Feb;9(2):359–366. doi: 10.1128/jvi.9.2.359-366.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Officer J. E., Tecson N., Estes J. D., Fontanilla E., Rongey R. W., Gardner M. B. Isolation of a neurotropic type C virus. Science. 1973 Sep 7;181(4103):945–947. doi: 10.1126/science.181.4103.945. [DOI] [PubMed] [Google Scholar]
- PERLMANN G. E. The nature of phosphorus linkages in phosphoproteins. Adv Protein Chem. 1955;10:1–30. doi: 10.1016/s0065-3233(08)60103-5. [DOI] [PubMed] [Google Scholar]
- Pal B. K., Wright M., Officer J. E., Gardner M. B., Roy-Burman P. Subviral components of a wild mouse embryo-derived type C oncornavirus. Virology. 1973 Nov;56(1):189–197. doi: 10.1016/0042-6822(73)90298-5. [DOI] [PubMed] [Google Scholar]
- Roy-Burman P. Deoxyribonucleic acid polymerase associated with feline leukemia and sarcoma viruses: properties of the enzyme and its product. Int J Cancer. 1971 May 15;7(3):409–415. doi: 10.1002/ijc.2910070306. [DOI] [PubMed] [Google Scholar]
- Roy-Burman P., Pal B. K., Gardner M. B. Structural polypeptides of primate derived type C RNA tumor viruses. Biochem Biophys Res Commun. 1974 Jan 23;56(2):543–551. doi: 10.1016/0006-291x(74)90877-8. [DOI] [PubMed] [Google Scholar]
- Shapiro A. L., Viñuela E., Maizel J. V., Jr Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem Biophys Res Commun. 1967 Sep 7;28(5):815–820. doi: 10.1016/0006-291x(67)90391-9. [DOI] [PubMed] [Google Scholar]
- Sokol F., Clark H. F. Phosphoproteins, structural components of rhabdoviruses. Virology. 1973 Mar;52(1):246–263. doi: 10.1016/0042-6822(73)90413-3. [DOI] [PubMed] [Google Scholar]
- Somers K. D., Kirsten W. H. Murine sarcoma virus: titration patterns and interference by a murine leukemia virus. Int J Cancer. 1969 Sep 15;4(5):697–704. doi: 10.1002/ijc.2910040516. [DOI] [PubMed] [Google Scholar]
- Strand M., August J. T. Protein kinase and phosphate acceptor proteins in Rauscher murine leukaemia virus. Nat New Biol. 1971 Sep 29;233(39):137–140. doi: 10.1038/newbio233137a0. [DOI] [PubMed] [Google Scholar]
- Summers D. F., Maizel J. V., Jr, Darnell J. E., Jr Evidence for virus-specific noncapsid proteins in poliovirus-infected HeLa cells. Proc Natl Acad Sci U S A. 1965 Aug;54(2):505–513. doi: 10.1073/pnas.54.2.505. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tan K. B., Sokol F. Virion-bound protein kinase in Semliki forest and Sindbis viruses. J Virol. 1974 Jun;13(6):1245–1253. doi: 10.1128/jvi.13.6.1245-1253.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todaro G. J., Sherr C. J., Benveniste R. E., Lieber M. M., Melnick J. L. Type C viruses of baboons: isolation from normal cell cultures. Cell. 1974 May;2(1):55–61. doi: 10.1016/0092-8674(74)90008-7. [DOI] [PubMed] [Google Scholar]