Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Mar;15(3):550–555. doi: 10.1128/jvi.15.3.550-555.1975

Interspersion of sequences in avian myeloblastosis virus rna that rapidly hybridize with leukemic chicken cell DNA.

W N Drohan, M Shoyab, R Wall, M A Baluda
PMCID: PMC354492  PMID: 163372

Abstract

Liquid hybridization of progressively smaller fragments (35S, 27S, 15.5S, 12.5S, and 8S) of poly(A)-selected avian myeloblastosis virus RNA with excess DNA from leukemic chicken myeloblasts revealed that all sizes of RNA contained sequences complementary to both slowly and rapidly hybridizing cellular DNA sequences. Apparently, the RNA sequences which hybridize rapidly with excesses of cellular DNA are not restricted to any one region of the avian myeloblastosis virus 35S RNA. Instead, they appear to be randomly distributed over the entire 35S avian myeloblastosis virus RNA molecule with some positioned within 200 nucleotides of the poly(A) tract at the 3' end of the RNA.

Full text

PDF
550

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baluda M. A., Nayak D. P. Incorporation of precursors into ribonucleic acid, protein, glycoprotein, and lipoprotein of avian myeloblastosis virions. J Virol. 1969 Nov;4(5):554–566. doi: 10.1128/jvi.4.5.554-566.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baluda M. A. Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses. Proc Natl Acad Sci U S A. 1972 Mar;69(3):576–580. doi: 10.1073/pnas.69.3.576. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop J. O. Molecular hybridization of ribonucleic acid with a large excess of deoxyribonucleic acid. Biochem J. 1972 Jan;126(1):171–185. doi: 10.1042/bj1260171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bishop J. O., Pemberton R., Baglioni C. Reiteration frequency of haemoglobin genes in the duck. Nat New Biol. 1972 Feb 23;235(60):231–234. doi: 10.1038/newbio235231a0. [DOI] [PubMed] [Google Scholar]
  5. Bishop J. O. The effect of genetic complexity on the time-course of ribonucleic acid-deoxyribonucleic acid hybridization. Biochem J. 1969 Aug;113(5):805–811. doi: 10.1042/bj1130805. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Britten R. J., Davidson E. H. Gene regulation for higher cells: a theory. Science. 1969 Jul 25;165(3891):349–357. doi: 10.1126/science.165.3891.349. [DOI] [PubMed] [Google Scholar]
  7. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  8. Davidson E. H., Britten R. J. Organization, transcription, and regulation in the animal genome. Q Rev Biol. 1973 Dec;48(4):565–613. doi: 10.1086/407817. [DOI] [PubMed] [Google Scholar]
  9. Dina D., Crippa M., Beccari E. Hybridization properties and sequence arrangement in a population of mRNAs. Nat New Biol. 1973 Mar 28;242(117):101–105. doi: 10.1038/newbio242101a0. [DOI] [PubMed] [Google Scholar]
  10. Dina D., Meza I., Crippa M. Relative positions of the "repetitive", "unique" and poly(A) fragments of mRNA. Nature. 1974 Apr 5;248(448):486–490. doi: 10.1038/248486a0. [DOI] [PubMed] [Google Scholar]
  11. Firtel R. A., Jacobson A., Lodish H. F. Isolation and hybridization kinetics of messenger RNA from Dictyostelium discoideum. Nat New Biol. 1972 Oct 25;239(95):225–228. doi: 10.1038/newbio239225a0. [DOI] [PubMed] [Google Scholar]
  12. Firtel R. A., Lodish H. F. A small nuclear precursor of messenger RNA in the cellular slime mold Dictyostelium discoideum. J Mol Biol. 1973 Sep 15;79(2):295–314. doi: 10.1016/0022-2836(73)90007-7. [DOI] [PubMed] [Google Scholar]
  13. Green M., Cartas M. The genome of RNA tumor viruses contains polyadenylic acid sequences. Proc Natl Acad Sci U S A. 1972 Apr;69(4):791–794. doi: 10.1073/pnas.69.4.791. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Greenberg J. R., Perry R. P. Hybridization properties of DNA sequences directing the synthesis of messenger RNA and heterogeneous nuclear RNA. J Cell Biol. 1971 Sep;50(3):774–786. doi: 10.1083/jcb.50.3.774. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hutton J. R., Wetmur J. G. Renaturation of bacteriophage phiX174 DNA-RNA hybrid: RNA length effect and nucleation rate constant. J Mol Biol. 1973 Jul 15;77(4):495–500. doi: 10.1016/0022-2836(73)90218-0. [DOI] [PubMed] [Google Scholar]
  16. Jelinek W., Adesnik M., Salditt M., Sheiness D., Wall R., Molloy G., Philipson L., Darnell J. E. Further evidence on the nuclear origin and transfer to the cytoplasm of polyadenylic acid sequences in mammalian cell RNA. J Mol Biol. 1973 Apr 15;75(3):515–532. doi: 10.1016/0022-2836(73)90458-0. [DOI] [PubMed] [Google Scholar]
  17. Kram R., Botchan M., Hearst J. E. Arrangement of the highly reiterated DNA sequences in the centric heterochromatin of Drosophila melanogaster. Evidence for interspersed spacer DNA. J Mol Biol. 1972 Feb 28;64(1):103–117. doi: 10.1016/0022-2836(72)90323-3. [DOI] [PubMed] [Google Scholar]
  18. Lai M. M., Duesberg P. H. Adenylic acid-rich sequence in RNAs of Rous sarcoma virus and Rauscher mouse leukaemia virus. Nature. 1972 Feb 18;235(5338):383–386. doi: 10.1038/235383c0. [DOI] [PubMed] [Google Scholar]
  19. Melli M., Whitfield C., Rao K. V., Richardson M., Bishop J. O. DNA-RNA hybridization in vast DNA excess. Nat New Biol. 1971 May 5;231(18):8–12. [PubMed] [Google Scholar]
  20. Neiman P. E. Rous sarcoma virus nucleotide sequences in cellular DNA: measurement by RNA-DNA hybridization. Science. 1972 Nov 17;178(4062):750–753. doi: 10.1126/science.178.4062.750. [DOI] [PubMed] [Google Scholar]
  21. Shoyab M., Baluda M. A., Evans R. Acquisition of new DNA sequences after infection of chicken cells with avian myeloblastosis virus. J Virol. 1974 Feb;13(2):331–339. doi: 10.1128/jvi.13.2.331-339.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Shoyab M., Markham P. D., Baluda M. A. Reliability of the RNA-DNA filter hybridization for the detection of oncornavirus-specific DNA sequences. J Virol. 1974 Aug;14(2):225–230. doi: 10.1128/jvi.14.2.225-230.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Verma I. M., Meuth N. L., Baltimore D. Covalent linkage between ribonucleic Acid primer and deoxyribonucleic Acid product of the avian myeloblastosis virus deoxyribonucleic Acid polymerase. J Virol. 1972 Oct;10(4):622–627. doi: 10.1128/jvi.10.4.622-627.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wu J. R., Hurn J., Bonner J. Size and distribution of the repetitive segments of the Drosophila genome. J Mol Biol. 1972 Feb 28;64(1):211–219. doi: 10.1016/0022-2836(72)90330-0. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES