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. 1975 Apr;15(4):707–713. doi: 10.1128/jvi.15.4.707-713.1975

RNA synthesis in cells infected with herpes simplex virus. X. Properties of viral symmetric transcripts and of double-stranded RNA prepared from them.

B Jacquemont, B Roizman
PMCID: PMC354512  PMID: 163916

Abstract

HEp-2 cells infected with herpes simplex 1 virus contain RNA transcripts capable of forming double-stranded (DS) RNA on annealing. The properties of purified DS RNA were as follows (i) DS RNA is resistant to depolymerization by RNase A or T-1 in 2 times 0.15 M NaCl, plus 0.015 M sodium citrate (SSC) but not 0.1 times SSC or following thermal denaturation. (ii) The Tm of the viral DS RNA was 100 C in 0.1 times SSC. (iii) Undenatured DS RNA does not hybridize with viral DNA: upon denaturation, excess unlabeled RNA drove 50 to 55% of labeled DNA into DNA-RNA hybrid. The kinetics of hybridization indicate that the DS RNA consists of at least two populations of transcripts arising from 29 and 26% of viral DNA and differing 40-fold in molar concentration.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aloni Y. Extensive symmetrical transcription of Simian Virus 40 DNA in virus-yielding cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2404–2409. doi: 10.1073/pnas.69.9.2404. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aloni Y., Locker H. Symmetrical in vivo transcription of polyoma DNA and the separation of self-complementary viral and cell RNA. Virology. 1973 Aug;54(2):495–505. doi: 10.1016/0042-6822(73)90159-1. [DOI] [PubMed] [Google Scholar]
  3. Billeter M. A., Weissmann C., Warner R. C. Replication of viral ribonucleic acid. IX. Properties of double-stranded RNA from Escherichia coli infected with bacteriophage MS2. J Mol Biol. 1966 May;17(1):145–173. doi: 10.1016/s0022-2836(66)80101-8. [DOI] [PubMed] [Google Scholar]
  4. Colby C., Duesberg P. H. Double-stranded RNA in vaccinia virus infected cells. Nature. 1969 Jun 7;222(5197):940–944. doi: 10.1038/222940a0. [DOI] [PubMed] [Google Scholar]
  5. Frenkel N., Roizman B., Cassai E., Nahmias A. A DNA fragment of Herpes simplex 2 and its transcription in human cervical cancer tissue. Proc Natl Acad Sci U S A. 1972 Dec;69(12):3784–3789. doi: 10.1073/pnas.69.12.3784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frenkel N., Roizman B. Ribonucleic acid synthesis in cells infected with herpes simplex virus: controls of transcription and of RNA abundance. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2654–2658. doi: 10.1073/pnas.69.9.2654. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kieff E., Hoyer B., Bachenheimer S., Roizman B. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J Virol. 1972 May;9(5):738–745. doi: 10.1128/jvi.9.5.738-745.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kozak M., Roizman B. RNA synthesis in cells infected with herpes simplex virus. IX. Evidence for accumulation of abundant symmetric transcripts in nuclei. J Virol. 1975 Jan;15(1):36–40. doi: 10.1128/jvi.15.1.36-40.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kozak M., Roizman B. Regulation of herpesvirus macromolecular synthesis: nuclear retention of nontranslated viral RNA sequences. Proc Natl Acad Sci U S A. 1974 Nov;71(11):4322–4326. doi: 10.1073/pnas.71.11.4322. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lucas J. J., Ginsberg H. S. Identification of double-stranded virus-specific ribonucleic acid in KB cells infected with type 2 adenovirus. Biochem Biophys Res Commun. 1972 Oct 6;49(1):39–44. doi: 10.1016/0006-291x(72)90006-x. [DOI] [PubMed] [Google Scholar]
  12. Rabin E. Z., Preiss B., Fraser M. J. A nuclease from Neurospora crassa conidia specific for single-stranded nucleic acids. Prep Biochem. 1971;1(4):283–307. doi: 10.1080/00327487108081946. [DOI] [PubMed] [Google Scholar]
  13. Roizman B., Spear P. G. Preparation of herpes simplex virus of high titer. J Virol. 1968 Jan;2(1):83–84. doi: 10.1128/jvi.2.1.83-84.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Silverstein S., Bachenheimer S. L., Frenkel N., Roizman B. Relationship between post-transcriptional adenylation of herpes virus RNA and messenger RNA abundance. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2101–2104. doi: 10.1073/pnas.70.7.2101. [DOI] [PMC free article] [PubMed] [Google Scholar]

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