Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1975 Apr;15(4):720–725. doi: 10.1128/jvi.15.4.720-725.1975

Growth studies of three phi chi 174 mutants in tsDNA mutants Escherichia coli.

S R Haworth, C F Gilgun, C E Dowell
PMCID: PMC354514  PMID: 1090749

Abstract

Three mutants of phi chi 174 were examined for their abilities to grow in temperature-sensitive dna,A, dnaC, dnaE, or dnaG mutants of Escherichia coli. The results indicate that the phage mutants have acquired the ability to grow in some tsDNA mutants that normally block the replication of wild-type phi chi 174. Evidence is presented indicating that the phage mutants contain one or more altered structural proteins. Several models are presented to explain how altered phage structural proteins could affect phi chi 174 replication.

Full text

PDF
720

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benbow R. M., Zuccarelli A. J., Davis G. C., Sinsheimer R. L. Genetic recombination in bacteriophage phi chi 174. J Virol. 1974 Apr;13(4):898–907. doi: 10.1128/jvi.13.4.898-907.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bone D. R., Dowel C. E. A mutant of bacteriophage øX174 which infects E. coli K12 strains. I. Isolation and partial characterization of øXtB. Virology. 1973 Apr;52(2):319–329. [PubMed] [Google Scholar]
  3. Bone D. R., Dowell C. E. A mutant of bacteriophage øX174 which infects E. coli K12 strains. II. Replication of øXtB in tsDNA strains. Virology. 1973 Apr;52(2):330–336. doi: 10.1016/0042-6822(73)90327-9. [DOI] [PubMed] [Google Scholar]
  4. Brutlag D., Schekman R., Kornberg A. A possible role for RNA polymerase in the initiation of M13 DNA synthesis. Proc Natl Acad Sci U S A. 1971 Nov;68(11):2826–2829. doi: 10.1073/pnas.68.11.2826. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Denhardt D. T., Iwaya M., McFadden G., Schochetman G. The mechanism of replication of phi X174 single-stranded DNA. VI. Requirements for ribonucleoside triphosphates and DNA polymerase 3. Can J Biochem. 1973 Dec;51(12):1588–1597. doi: 10.1139/o73-214. [DOI] [PubMed] [Google Scholar]
  6. Denhardt D. T., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. 3. Phage maturation and lysis after synchronized infection. J Mol Biol. 1965 Jul;12(3):641–646. doi: 10.1016/s0022-2836(65)80318-7. [DOI] [PubMed] [Google Scholar]
  7. Doniger J., Tessman I. An S13 capsid mutant that makes no replicative-form DNA. Virology. 1969 Nov;39(3):389–394. doi: 10.1016/0042-6822(69)90086-5. [DOI] [PubMed] [Google Scholar]
  8. Dowell C. E. Cold-sensitive mutants of bacteriophage phi-X-174. I. A mutant blocked in the eclipse function at low temperature. Proc Natl Acad Sci U S A. 1967 Sep;58(3):958–961. doi: 10.1073/pnas.58.3.958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Dowell C. E., Sinsheimer R. L. The process of infection with bacteriophage phi-X174. IX. Studies on the physiology of three phi-X174 temperature-sensitive mutants. J Mol Biol. 1966 Apr;16(2):374–386. doi: 10.1016/s0022-2836(66)80180-8. [DOI] [PubMed] [Google Scholar]
  10. Dumas L. B., Miller C. A. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaE mutant of Escherichia coli C. J Virol. 1973 Jun;11(6):848–855. doi: 10.1128/jvi.11.6.848-855.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Greenlee L. L. Replication of bacteriophage phiX-174 in a mutant of Escherichia coli defective in the dnaE gene. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1757–1760. doi: 10.1073/pnas.70.6.1757. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Henry T. J., Knippers R. Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549–1553. doi: 10.1073/pnas.71.4.1549. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Iwaya M., Denhardt D. T. The mechanism of replication of phi X174 single-stranded DNA. II. The role of viral proteins. J Mol Biol. 1971 Apr 28;57(2):159–175. doi: 10.1016/0022-2836(71)90339-1. [DOI] [PubMed] [Google Scholar]
  14. Kranias E. G., Dumas L. B. Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C. J Virol. 1974 Jan;13(1):146–154. doi: 10.1128/jvi.13.1.146-154.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Newbold J. E., Sinsheimer R. L. The process of infection with bacteriophage phiX174. XXXII. Early steps in the infection process: attachment, eclipse and DNA penetration. J Mol Biol. 1970 Apr 14;49(1):49–66. doi: 10.1016/0022-2836(70)90375-x. [DOI] [PubMed] [Google Scholar]
  16. Schaller H., Otto B., Nüsslein V., Huf J., Herrmann R., Bonhoeffer F. Deoxyribonucleic acid replication in vitro. J Mol Biol. 1972 Jan 28;63(2):183–200. doi: 10.1016/0022-2836(72)90369-5. [DOI] [PubMed] [Google Scholar]
  17. Schekman R., Wickner W., Westergaard O., Brutlag D., Geider K., Bertsch L. L., Kornberg A. Initiation of DNA synthesis: synthesis of phiX174 replicative form requires RNA synthesis resistant to rifampicin. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2691–2695. doi: 10.1073/pnas.69.9.2691. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Sinsheimer R. L. Bacteriophage phi-X174 and related viruses. Prog Nucleic Acid Res Mol Biol. 1968;8:115–169. [PubMed] [Google Scholar]
  19. Tessman I., Tessman E. S. Functional units of phage S13: identification of two genes that determine the structure of the phage coat. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1459–1462. doi: 10.1073/pnas.55.6.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wechsler J. A., Gross J. D. Escherichia coli mutants temperature-sensitive for DNA synthesis. Mol Gen Genet. 1971;113(3):273–284. doi: 10.1007/BF00339547. [DOI] [PubMed] [Google Scholar]
  21. Wickner R. B., Wright M., Wickner S., Hurwitz J. Conversion of phiX174 and fd single-stranded DNA to replicative forms in extracts of Escherichia coli. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3233–3237. doi: 10.1073/pnas.69.11.3233. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES