Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Virology logoLink to Journal of Virology
. 1975 Apr;15(4):726–732. doi: 10.1128/jvi.15.4.726-732.1975

Structure and function of herpesvirus genomes. I. comparison of five HSV-1 and two HSV-2 strains by cleavage their DNA with eco R I restriction endonuclease.

J Skare, W P Summers, W C Summers
PMCID: PMC354515  PMID: 163917

Abstract

The restriction endonuclease Eco R I cleaves HSV-1 and HSV-2 DNA into specific fragments that can be resolved by agarose gel electrophoresis. Comparison of HSV-1 strains KOS, 14-012, MP, F, and CI 101, and HSV-2 strains 333 and 186, suggests that the DNAs from type 1 strains are similar but not identical, and that the type 2 strains differ greatly from type 1 strains. The molecular lengths of the fragments have been determined by electron microscopy and can be used to calibrate gel electrophoretic analyses of DNA fragments.

Full text

PDF
726

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aaij C., Borst P. The gel electrophoresis of DNA. Biochim Biophys Acta. 1972 May 10;269(2):192–200. doi: 10.1016/0005-2787(72)90426-1. [DOI] [PubMed] [Google Scholar]
  2. Allet B., Jeppesen P. G., Katagiri K. J., Delius H. Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI. Nature. 1973 Jan 12;241(5385):120–123. doi: 10.1038/241120a0. [DOI] [PubMed] [Google Scholar]
  3. Bronson D. L., Dreesman G. R., Biswal N., Benyesh-Melnick M. Defective virions of herpes simplex viruses. Intervirology. 1973;1(3):141–153. doi: 10.1159/000148841. [DOI] [PubMed] [Google Scholar]
  4. DUBBS D. R., KIT S. MUTANT STRAINS OF HERPES SIMPLEX DEFICIENT IN THYMIDINE KINASE-INDUCING ACTIVITY. Virology. 1964 Apr;22:493–502. doi: 10.1016/0042-6822(64)90070-4. [DOI] [PubMed] [Google Scholar]
  5. Darai G., Munk K. Human embryonic lung cells abortively infected with Herpes virus hominis type 2 show some properties of cell transformation. Nat New Biol. 1973 Feb 28;241(113):268–269. doi: 10.1038/newbio241268a0. [DOI] [PubMed] [Google Scholar]
  6. Duff R., Rapp F. Oncogenic transformation of hamster cells after exposure to herpes simplex virus type 2. Nat New Biol. 1971 Sep 8;233(36):48–50. doi: 10.1038/newbio233048a0. [DOI] [PubMed] [Google Scholar]
  7. Duff R., Rapp F. Oncogenic transformation of hamster embryo cells after exposure to inactivated herpes simplex virus type 1. J Virol. 1973 Aug;12(2):209–217. doi: 10.1128/jvi.12.2.209-217.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ejercito P. M., Kieff E. D., Roizman B. Characterization of herpes simplex virus strains differing in their effects on social behaviour of infected cells. J Gen Virol. 1968 May;2(3):357–364. doi: 10.1099/0022-1317-2-3-357. [DOI] [PubMed] [Google Scholar]
  9. Godson G. N., Boyer H. Susceptibility of the phiX-like phages G4 and G14 to R-EcoRi endonuclease. Virology. 1974 Nov;62(1):270–275. doi: 10.1016/0042-6822(74)90321-3. [DOI] [PubMed] [Google Scholar]
  10. Godson G. N. Evolution of phi-chi 174. Isolation of four new phi-chi-like phages and comparison with phi-chi 174. Virology. 1974 Mar;58(1):272–289. doi: 10.1016/0042-6822(74)90161-5. [DOI] [PubMed] [Google Scholar]
  11. HOGGAN M. D., ROIZMAN B. The isolation and properties of a variant of Herpes simplex producing multinucleated giant cells in monolayer cultures in the presence of antibody. Am J Hyg. 1959 Sep;70:208–219. doi: 10.1093/oxfordjournals.aje.a120071. [DOI] [PubMed] [Google Scholar]
  12. Hayward G. S., Smith M. G. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J Mol Biol. 1972 Feb 14;63(3):383–395. doi: 10.1016/0022-2836(72)90435-4. [DOI] [PubMed] [Google Scholar]
  13. House J. A. Bovine herpesvirus IBR-IPV. Strain differences. Cornell Vet. 1972 Jul;62(3):431–453. [PubMed] [Google Scholar]
  14. Kieff E., Hoyer B., Bachenheimer S., Roizman B. Genetic relatedness of type 1 and type 2 herpes simplex viruses. J Virol. 1972 May;9(5):738–745. doi: 10.1128/jvi.9.5.738-745.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rawls W. E., Laurel D., Melnick J. L., Glicksman J. M., Kaufman R. H. A search for viruses in smegma, premalignant and early malignant cervical tissues. The isolation of Herpesviruses with distinct antigenic properties. Am J Epidemiol. 1968 May;87(3):647–655. doi: 10.1093/oxfordjournals.aje.a120855. [DOI] [PubMed] [Google Scholar]
  16. SMITH K. O. RELATIONSHIP BETWEEN THE ENVELOPE AND THE INFECTIVITY OF HERPES SIMPLEX VIRUS. Proc Soc Exp Biol Med. 1964 Mar;115:814–816. doi: 10.3181/00379727-115-29045. [DOI] [PubMed] [Google Scholar]
  17. Schaffer P. A., Aron G. M., Biswal N., Benyesh-Melnick M. Temperature-sensitive mutants of herpes simplex virus type 1: isolation, complementation and partial characterization. Virology. 1973 Mar;52(1):57–71. doi: 10.1016/0042-6822(73)90398-x. [DOI] [PubMed] [Google Scholar]
  18. Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
  19. Sheldrick P., Berthelot N. Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):667–678. doi: 10.1101/sqb.1974.039.01.080. [DOI] [PubMed] [Google Scholar]
  20. Smith H. O., Nathans D. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J Mol Biol. 1973 Dec 15;81(3):419–423. doi: 10.1016/0022-2836(73)90152-6. [DOI] [PubMed] [Google Scholar]
  21. Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
  22. Summers W. C. The process of infection with coliphage T7. I. Characterization of T7 RNA by polyacrylamide gel electrophoretic analysis. Virology. 1969 Oct;39(2):175–181. doi: 10.1016/0042-6822(69)90037-3. [DOI] [PubMed] [Google Scholar]
  23. Timbury M. C., Subak-Sharpe J. H. Genetic interactions between temperature-sensitive mutants of types 1 and 2 herpes simplex viruses. J Gen Virol. 1973 Mar;18(3):347–357. doi: 10.1099/0022-1317-18-3-347. [DOI] [PubMed] [Google Scholar]
  24. Wagner E. K., Tewari K. K., Kolodner R., Warner R. C. The molecular size of the herpes simplex virus type 1 genome. Virology. 1974 Feb;57(2):436–447. doi: 10.1016/0042-6822(74)90183-4. [DOI] [PubMed] [Google Scholar]
  25. Wagner M., Skare J., Summers W. C. Analysis of DNA of defective herpes simplex virus type 1 by restriction endonuclease cleavage and nucleic acid hybridization. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 2):683–686. doi: 10.1101/sqb.1974.039.01.082. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES