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. 1975 Apr;15(4):855–860. doi: 10.1128/jvi.15.4.855-860.1975

Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. II. Location of the structural gene for thymidine kinase.

K V Chace, D H Hall
PMCID: PMC354529  PMID: 1090751

Abstract

Amber mutants of bacteriophage T4 have been isolated that induce thymidine kinase activity only after infection of a strain of Escherichia coli carrying a suppressor mutation. The activity induced when one of these mutants infected this suppressor strain is much more heat sensitive than the activity induced by wild-type T4. This indicates that this amber mutation lies within the structural gene for thymidine kinase. This gene is between fI and v on the standard T4 genetic map. A mutant of tt4 that is unable to induce thymidine kinase activity incorporates only about one-eighth as much thymidine into its DNA as phage that do induce thymidine kinase. This contrasts to the findings that the total thymidine kinase activity in extracts prepared from cells infected with phage able to induce thymidine kinase in only twice as great as the activity in cells infected with the mutant unable to induce the enzyme.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chace K. V., Hall D. H. Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity. J Virol. 1973 Aug;12(2):343–348. doi: 10.1128/jvi.12.2.343-348.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
  3. Goscin L. A., Hall D. H. Hydroxyurea-sensitive mutants of bacteriophage T4. Virology. 1972 Oct;50(1):84–94. doi: 10.1016/0042-6822(72)90348-0. [DOI] [PubMed] [Google Scholar]
  4. Hall D. H. Mutants of bacteriophage T4 unable to induce dihydrofolate reductase activity. Proc Natl Acad Sci U S A. 1967 Aug;58(2):584–591. doi: 10.1073/pnas.58.2.584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall D. H., Tessman I., Karlström O. Linkage of T4 genes controlling a series of steps in pyrimidine biosynthesis. Virology. 1967 Mar;31(3):442–448. doi: 10.1016/0042-6822(67)90224-3. [DOI] [PubMed] [Google Scholar]
  6. Hall D. H., Tessman I. T4 mutants unable to induce deoxycytidylate deaminase activity. Virology. 1966 Jun;29(2):339–345. doi: 10.1016/0042-6822(66)90041-9. [DOI] [PubMed] [Google Scholar]
  7. Igarashi K., Hiraga S., Yura T. A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80. Genetics. 1967 Nov;57(3):643–654. doi: 10.1093/genetics/57.3.643. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Koerner J. F. Enzymes of nucleic acid metabolism. Annu Rev Biochem. 1970;39:291–322. doi: 10.1146/annurev.bi.39.070170.001451. [DOI] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Price A. R., Warner H. R. A structural gene for bacteriophage T4-induced deoxycytidine triphosphate-deoxyuridine triphosphage nucleotidohydrolase. Virology. 1968 Nov;36(3):523–526. doi: 10.1016/0042-6822(68)90183-9. [DOI] [PubMed] [Google Scholar]
  11. Ritchie D. A., Jamieson A. T., White F. E. The induction of deoxythymidine kinase by bacteriophage T4. J Gen Virol. 1974 Jul;24(1):115–127. doi: 10.1099/0022-1317-24-1-115. [DOI] [PubMed] [Google Scholar]
  12. SIMON E. H., TESSMAN I. THYMIDINE-REQUIRING MUTANTS OF PHAGE T4. Proc Natl Acad Sci U S A. 1963 Sep;50:526–532. doi: 10.1073/pnas.50.3.526. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Short E. V., Jr, Warner H. R., Koerner J. F. The effect of cupric ions on the indole reaction for the determination of deoxyribonucleic acid. J Biol Chem. 1968 Jun 25;243(12):3342–3344. [PubMed] [Google Scholar]
  14. Tessman I., Greenberg D. B. Ribonucleotide reductase genes of phage T4: map location of the thioredoxin gene nrdC. Virology. 1972 Jul;49(1):337–338. doi: 10.1016/s0042-6822(72)80040-0. [DOI] [PubMed] [Google Scholar]
  15. WEIGERT M. G., GAREN A. AMINO ACID SUBSTITUTIONS RESULTING FROM SUPPRESSION OF NONSENSE MUTATIONS. I. SERINE INSERTION BY THE SU-1 SUPPRESSOR GENE. J Mol Biol. 1965 Jun;12:448–455. doi: 10.1016/s0022-2836(65)80267-4. [DOI] [PubMed] [Google Scholar]
  16. Yeh Y. C., Dubovi E. J., Tessman I. Control of pyrimidine biosynthesis by phage T4: mutants unable to catalyze the reduction of cytidine diphosphate. Virology. 1969 Apr;37(4):615–623. doi: 10.1016/0042-6822(69)90279-7. [DOI] [PubMed] [Google Scholar]
  17. Yeh Y. C., Tessman I. Control of pyrimidine biosynthesis by phage T4. II. In vitro complementation between ribonucleotide reductase mutants. Virology. 1972 Mar;47(3):767–772. doi: 10.1016/0042-6822(72)90567-3. [DOI] [PubMed] [Google Scholar]

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