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. 1975 Apr;15(4):1037–1041. doi: 10.1128/jvi.15.4.1037-1041.1975

Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells.

J Symington, M J Schlesinger
PMCID: PMC354551  PMID: 1167912

Abstract

A variant of Sindbis virus has been isolated by growing a stock of virus, previously passaged on chicken embryo cells, in mouse plasmacytoma (MOPC 315) cells in suspension culture. An indirect immunofluorescence test and infective center assay showed that only a small fraction of cells could be infected by the stock wild-type virus, but that the population of virus accumulating after a few passages on the mouse cells had host-range properties distinct from the stock virus. The mouse-passaged virus retained its virulence for the original host and back-passaging of this virus on chicken cells did not change its newly acquired properties. Thus, this variant appears to be a genetically distinct form of Sindbis that adsorbs to and grows much better than the stock virus on several types of mouse cells including cultures of mouse macrophages.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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