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. 1975 May;15(5):1168–1175. doi: 10.1128/jvi.15.5.1168-1175.1975

Heat-stable variant of human adenovirus type 5: characterization and use in three-factor crosses.

C S Young
PMCID: PMC354571  PMID: 1142474

Abstract

A variant of human adenovirus type 5 which is heat stable (hs) in vitro has been isolated following three rounds of heat inactivation at 52 C. The variant is genetically stable, both through vegetative viral passage and through recombination into other genetic backgrounds, which suggests that it arises from a single mutation. Three-factor crosses, using this mutant in conjunction with previously described temperature-sensitive mutants, suggest the hs mutation lies near the left-hand end of the genetic map. The mutant has been used to demonstrate the production of reciprocal recombinants in two-factor crosses. The mutational lesion is unknown, but phenotypic mixing occurs in hs times hs-+ infections, which suggests that it lies in a gene specifying a viri on structural protein. Other biological parameters examined have shown no differences from the wild-type hs-+.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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