Abstract
A previous paper (1973) indicated that infection with bacteriophage R17 permits the synthesis of RNA and spermidine in Escherichia coli (CP78 in the absence of the exogenous essential amino acid, arginine. We have now isolated RNA formed under such conditions and analyzed the newly synthesized species by agarose-acrylamide electrophoresis. It has been shown that infection of the stringent cells in the absence of exogenous arginine resulted in a marked incorporation of uracil into rRNA, as well as into R17 RNA. It was shown that, although the organism was nonauxotrophic for uracil, addition of [-14C]uracil resulted in the rapid formation of TUP, the specific radioactivity of which approached that of the exogenous uracil. This indicated that the incorporation of exogenous uracil into rRNA in R17 infection of the stringent strain reflected a true stimulated synthesis of this nucleic acid. Infection of the essentially isogenic relaxed strain, CP79, under the same conditions inhibited the RNA synthesis to a much less extent than the inhibition caused during the normal infection. These observations provide another example of the close correlation between synthesis of spermidine and of host RNA, even in cells infected by an RNA bacteriophage.
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Selected References
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- BOREK E., RYAN A., ROCKENBACH J. Nucleic acid metabolism in relation to the lysogenic phenomenon. J Bacteriol. 1955 Apr;69(4):460–467. doi: 10.1128/jb.69.4.460-467.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cashel M., Kalbacher B. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response. J Biol Chem. 1970 May 10;245(9):2309–2318. [PubMed] [Google Scholar]
- Fukuma I., Cohen S. S. Polyamine synthesis and accumulation in Escherichia coli infected with bacteriophage R17. J Virol. 1973 Dec;12(6):1259–1264. doi: 10.1128/jvi.12.6.1259-1264.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Munch-Petersen B., Tyrsted G., Dupont B. The deoxyribonucleoside 5'-triphosphate (dATP and dTTP) pool in phytohemagglutinin-stimulated and non-stimulated human lymphocytes. Exp Cell Res. 1973 Jun;79(2):249–256. doi: 10.1016/0014-4827(73)90442-4. [DOI] [PubMed] [Google Scholar]
- Peacock A. C., Dingman C. W. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry. 1968 Feb;7(2):668–674. doi: 10.1021/bi00842a023. [DOI] [PubMed] [Google Scholar]
- Plunkett W., Lapi L., Ortiz P. J., Cohen S. S. Penetration of mouse fibroblasts by the 5'-phosphate of 9-beta-D-arabinofuranosyladenine and incorporation of the nucleotide into DNA. Proc Natl Acad Sci U S A. 1974 Jan;71(1):73–77. doi: 10.1073/pnas.71.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raina A., Cohen S. S. Polyamines and RNA synthesis in a polyauxotrophic strain of E. coli. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1587–1593. doi: 10.1073/pnas.55.6.1587. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Raina A., Jansen M., Cohen S. S. Polyamines and the accumulation of ribonucleic acid in some polyauxotrophic strains of Escherichia coli. J Bacteriol. 1967 Nov;94(5):1684–1696. doi: 10.1128/jb.94.5.1684-1696.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryan A. M., Borek E. The relaxed control phenomenon. Prog Nucleic Acid Res Mol Biol. 1971;11:193–228. doi: 10.1016/s0079-6603(08)60328-1. [DOI] [PubMed] [Google Scholar]
- STENT G. S., BRENNER S. A genetic locus for the regulation of ribonucleic acid synthesis. Proc Natl Acad Sci U S A. 1961 Dec 15;47:2005–2014. doi: 10.1073/pnas.47.12.2005. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Seiler N., Werner G., Fischer H. A., von Bernd Knötgen M., Hinz H. Der Einfluss von Nahrungsentzug, Phenobarbital und Perfluorbuttersäure auf den Nucleinsäuren-, Spermin- und Spermidingehalt der Mäuseleber. Hoppe Seylers Z Physiol Chem. 1969 Jun;350(6):676–682. [PubMed] [Google Scholar]