Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 May;15(5):1214–1221. doi: 10.1128/jvi.15.5.1214-1221.1975

Simian Virus 40 DNA Segment of the Adenovirus 7-Simian Virus 40 Hybrid, E46+, and Its Transcription During Permissive Infection of Monkey Kidney Cells

Paul Lebowitz *, George Khoury 1
PMCID: PMC354576  PMID: 16789154

Abstract

Nucleic acid hybridization methodology has been used to investigate the span of the simian virus 40 (SV40) DNA segment in the adenovirus 7-SV40 hybrid, E46+, and the extent of its transcription in lytically infected monkey kidney cells. The SV40 segment of E46+ comprises approximately 62% of the SV40 genome; it originates in the proximal region of Hin-G (the G fragment derived by cleavage of intact SV40 DNA with Haemophilus influenzae restriction endonuclease), extends sequentially through approximately 80% of this fragment, all of fragments Hin-B, -I, -H, and -A, and terminates approximately 70% of the distance through Hin-C. During E46+ lytic infection of permissive cells, the vast majority of stable cytoplasmic SV40-specific RNA is transcribed from the minus (E) strand of the fragments Hin-A, -H, -I, and -B, comprising the early template region. Transcripts of the minus strand of the Hin-G and -C fragments are detected in much lower concentrations, especially in the total lytic cellular RNA, whereas RNA complementary to the plus (L) strand is not detected. The transcriptional pattern of the SV40 segment within E46+ is thus very similar to that in a number of transformed cell lines and in some respects to the transcriptional pattern in a series of nondefective adenovirus 2-SV40 hybrid viruses. These results suggest a common transcriptional mechanism for integrated SV40 DNA.

Full text

PDF
1214

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baum S. G., Fox R. I. Components of adenovirus-simian virus 40 hybrid viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1525–1529. doi: 10.1073/pnas.68.7.1525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baum S. G., Reich P. R., Hybner C. J., Rowe W. P., Weissman S. M. Biophysical evidence for linkage of adenovirus and SV40 DNA's in adenovirus 7-SV40 hybrid particles. Proc Natl Acad Sci U S A. 1966 Nov;56(5):1509–1515. doi: 10.1073/pnas.56.5.1509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Danna K. J., Sack G. H., Jr, Nathans D. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome. J Mol Biol. 1973 Aug 5;78(2):363–376. doi: 10.1016/0022-2836(73)90122-8. [DOI] [PubMed] [Google Scholar]
  4. Danna K., Nathans D. Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae. Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913–2917. doi: 10.1073/pnas.68.12.2913. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dhar R., Zain S., Weissman S. M., Pan J., Subramanian K. Nucleotide sequences of RNA transcribed in infected cells and by Escherichia coli RNA polymerase from a segment of simian virus 40 DNA. Proc Natl Acad Sci U S A. 1974 Feb;71(2):371–375. doi: 10.1073/pnas.71.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  8. Henry P. H., Schnipper L. E., Samaha R. J., Crumpacker C. S., Lewis A. M., Jr, Levine A. S. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. VI. Characterization of the DNA from five nondefective hybrid viruses. J Virol. 1973 May;11(5):665–671. doi: 10.1128/jvi.11.5.665-671.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
  10. Kelly T. J., Jr, Lewis A. M., Jr Use of nondefective adenovirus-simian virus 40 hybrids for mapping the simian virus 40 genome. J Virol. 1973 Sep;12(3):643–652. doi: 10.1128/jvi.12.3.643-652.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kelly T. J., Jr, Rose J. A. Simian virus 40 integration site in an adenovirus 7-simian virus 40 hybrid DNA molecule. Proc Natl Acad Sci U S A. 1971 May;68(5):1037–1041. doi: 10.1073/pnas.68.5.1037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kelly T. J. Structure of the DNA of the adenovirus 7-simian virus 40 hybrid, e46, by electron microscopy. J Virol. 1975 May;15(5):1267–1272. doi: 10.1128/jvi.15.5.1267-1272.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Khoury G., Byrne J. C., Martin M. A. Patterns of Simian Virus 40 DNA transcription after acute infection of permissive and nonpermissive cells. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1925–1928. doi: 10.1073/pnas.69.7.1925. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Khoury G., Byrne J. C., Takemoto K. K., Martin M. A. Patterns of simian virus 40 deoxyribonucleic acid transcription. II. In transformed cells. J Virol. 1973 Jan;11(1):54–60. doi: 10.1128/jvi.11.1.54-60.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Khoury G., Lewis A. M., Jr, Oxman M. N., Levine A. S. Strand orientation of SV40 transcription in cells infected by non-defective adenovirus 2-SV40 hybrid viruses. Nat New Biol. 1973 Dec 19;246(155):202–205. doi: 10.1038/newbio246202a0. [DOI] [PubMed] [Google Scholar]
  16. Khoury G., Martin M. A., Lee T. N., Danna K. J., Nathans D. A map of simian virus 40 transcription sites expressed in productively infected cells. J Mol Biol. 1973 Aug 5;78(2):377–389. doi: 10.1016/0022-2836(73)90123-x. [DOI] [PubMed] [Google Scholar]
  17. Lebowitz P., Kelly T. J., Jr, Nathans D., Lee T. N., Lewis A. M., Jr A colinear map relating the simian virus 40 (SV40) DNA segments of six adenovirus-SV40 hybrids to the DNA fragments produced by restriction endonuclease cleavage of SV40 DNA. Proc Natl Acad Sci U S A. 1974 Feb;71(2):441–445. doi: 10.1073/pnas.71.2.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Levin M. J., Crumpacker C. S., Lewis A. M., Jr, Oxman M. N., Henry P. H., Rowe W. P. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. II. Relationship of adenovirus 2 deoxyribonucleic acid and simian virus 40 deoxyribonucleic acid in the Ad2+ND genome. J Virol. 1971 Mar;7(3):343–351. doi: 10.1128/jvi.7.3.343-351.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Lewis A. M., Jr, Levin M. J., Wiese W. H., Crumpacker C. S., Henry P. H. A nondefective (competent) adenovirus-SV40 hybrid isolated from the AD.2-SV40 hybrid population. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1128–1135. doi: 10.1073/pnas.63.4.1128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Lewis A. M., Jr, Levine A. S., Crumpacker C. S., Levin M. J., Samaha R. J., Henry P. H. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. V. Isolation of additional hybrids which differ in their simian virus 40-specific biological properties. J Virol. 1973 May;11(5):655–664. doi: 10.1128/jvi.11.5.655-664.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Lindstrom D. M., Dulbecco R. Strand orientation of simian virus 40 transcription in productively infected cells. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1517–1520. doi: 10.1073/pnas.69.6.1517. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Morrow J. F., Berg P., Kelly T. J., Jr, Lewis A. M., Jr Mapping of simian virus 40 early functions on the viral chromosome. J Virol. 1973 Sep;12(3):653–658. doi: 10.1128/jvi.12.3.653-658.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Oda K., Dulbecco R. Regulation of transcription of the SV40 DNA in productively infected and in transformed cells. Proc Natl Acad Sci U S A. 1968 Jun;60(2):525–532. doi: 10.1073/pnas.60.2.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Oxman M. N., Levin M. J., Lewis A. M., Jr Control of simian virus 40 gene expression in adenovirus-simian virus 40 hybrid viruses. Synthesis of hybrid adenovirus 2-simian virus 40 RNA molecules in cells infected with a nondefective adenovirus 2-simian virus 40 hybrid virus. J Virol. 1974 Feb;13(2):322–330. doi: 10.1128/jvi.13.2.322-330.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Ozanne B., Sharp P. A., Sambrook J. Transcription of simian virus 40. II. Hybridization of RNA extracted from different lines of transformed cells to the separated strands of simian virus 40 DNA. J Virol. 1973 Jul;12(1):90–98. doi: 10.1128/jvi.12.1.90-98.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Penman S. RNA metabolism in the HeLa cell nucleus. J Mol Biol. 1966 May;17(1):117–130. doi: 10.1016/s0022-2836(66)80098-0. [DOI] [PubMed] [Google Scholar]
  27. ROWE W. P., BAUM S. G. EVIDENCE FOR A POSSIBLE GENETIC HYBRID BETWEEN ADENOVIRUS TYPE 7 AND SV40 VIRUSES. Proc Natl Acad Sci U S A. 1964 Dec;52:1340–1347. doi: 10.1073/pnas.52.6.1340. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Rose J. A., Reich P. R., Weissman S. M. RNA production in adenovirus-infected KB cells. Virology. 1965 Dec;27(4):571–579. doi: 10.1016/0042-6822(65)90183-2. [DOI] [PubMed] [Google Scholar]
  30. Rowe W. P., Bau S. G. Studies of adenovirus SV40 hybrid viruses. II. Defectiveness of the hybrid particles. J Exp Med. 1965 Nov 1;122(5):955–966. doi: 10.1084/jem.122.5.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Sambrook J., Sharp P. A., Keller W. Transcription of Simian virus 40. I. Separation of the strands of SV40 DNA and hybridization of the separated strands to RNA extracted from lytically infected and transformed cells. J Mol Biol. 1972 Sep 14;70(1):57–71. doi: 10.1016/0022-2836(72)90163-5. [DOI] [PubMed] [Google Scholar]
  32. Sambrook J., Sugden B., Keller W., Sharp P. A. Transcription of simian virus 40. 3. Mapping of "early" and "late" species of RNA. Proc Natl Acad Sci U S A. 1973 Dec;70(12):3711–3715. doi: 10.1073/pnas.70.12.3711. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sambrook J., Westphal H., Srinivasan P. R., Dulbecco R. The integrated state of viral DNA in SV40-transformed cells. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1288–1295. doi: 10.1073/pnas.60.4.1288. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Smith H. O., Wilcox K. W. A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J Mol Biol. 1970 Jul 28;51(2):379–391. doi: 10.1016/0022-2836(70)90149-x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES