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. 1975 Jun;15(6):1323–1331. doi: 10.1128/jvi.15.6.1323-1331.1975

Biological and physical modifications of a murine oncornavirus by 2-deoxy-D-glucose.

E V Prochownik, S Panem, W H Kirsten
PMCID: PMC354599  PMID: 49438

Abstract

2-Deoxy-D-glucose (2-DG) inhibited the release of transforming Kirsten murine sarcoma-leukemia virus [KiMSV(KiMuLV)] from transformed rat kidney (NRK-K) cells. At a concentration of 30 mM 2-DG, RNA synthesis in NRK-K cells was inhibited by approximately 30 percent and protein synthesis was inhibited by as much as 80 percent of control levels. RNA synthesis was not inhibited in nontransformed normal rat kidney (NRK) cells, although protein synthesis was equally suppressed in NRK and NRK-K cells. After treatment with 2-DG, the release of physical particles of KiMSV(KiMuLV) from NRK-K cels was not reduced as determined by equilibrium density gradient centrifugation and assays for RNA-dependent DNA polymerase of culture fluids. The ability to detect virion-associated radioactivity in equilibrium density gradients was dependent on the conditions of labeling. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of KiMSV(KiMulLV) proteins revealed marked structural alterations after propagation of the virus in 30 mM 2-DG. These alterations may account for the observed loss of transforming ability of KiMSV(KiMuLV).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D. RNA-dependent DNA polymerase in virions of RNA tumour viruses. Nature. 1970 Jun 27;226(5252):1209–1211. doi: 10.1038/2261209a0. [DOI] [PubMed] [Google Scholar]
  2. Courtney R. J., Steiner S. M., Benyesh-Melnick M. Effects of 2-deoxy-D-glucose on herpes simplex virus replication. Virology. 1973 Apr;52(2):447–455. doi: 10.1016/0042-6822(73)90340-1. [DOI] [PubMed] [Google Scholar]
  3. Fairbanks G., Jr, Levinthal C., Reeder R. H. Analysis of C14-labeled proteins by disc electrophoresis. Biochem Biophys Res Commun. 1965 Aug 16;20(4):393–399. doi: 10.1016/0006-291x(65)90589-9. [DOI] [PubMed] [Google Scholar]
  4. Fairbanks G., Steck T. L., Wallach D. F. Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry. 1971 Jun 22;10(13):2606–2617. doi: 10.1021/bi00789a030. [DOI] [PubMed] [Google Scholar]
  5. Goodman N. C., Spiegelman S. Distinguishing reverse transcriptase of an RNA tumor virus from other known DNA polymerases. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2203–2206. doi: 10.1073/pnas.68.9.2203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hartley J. W., Rowe W. P. Production of altered cell foci in tissue culture by defective Moloney sarcoma virus particles. Proc Natl Acad Sci U S A. 1966 Apr;55(4):780–786. doi: 10.1073/pnas.55.4.780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hatanaka M., Augl C., Gilden R. V. Evidence for a functional change in the plasma membrane of murine sarcoma virus-infected mouse embryo cells. Transport and transport-associated phosphorylation of 14C-2-deoxy-D-glucose. J Biol Chem. 1970 Feb 25;245(4):714–717. [PubMed] [Google Scholar]
  8. Hunter E., Friis R. R., Vogt P. K. Inhibition of avian sarcoma virus replication by glucosamine. Virology. 1974 Apr;58(2):449–456. doi: 10.1016/0042-6822(74)90079-8. [DOI] [PubMed] [Google Scholar]
  9. Kaluza G., Schmidt M. F., Scholtissek C. Effect of 2-deoxy-D-glucose on the multiplication of Semliki Forest virus and the reversal of the block by mannose. Virology. 1973 Jul;54(1):179–189. doi: 10.1016/0042-6822(73)90127-x. [DOI] [PubMed] [Google Scholar]
  10. Keppler D. O., Rudigier J. F., Bischoff E., Decker K. F. The trapping of uridine phosphates by D-galactosamine. D-glucosamine, and 2-deoxy-D-galactose. A study on the mechanism of galactosamine hepatitis. Eur J Biochem. 1970 Dec;17(2):246–253. doi: 10.1111/j.1432-1033.1970.tb01160.x. [DOI] [PubMed] [Google Scholar]
  11. Klenk H. D., Scholtissek C., Rott R. Inhibition of glycoprotein biosynthesis of influenza virus by D-glucosamine and 2-deoxy-D-glucose. Virology. 1972 Sep;49(3):723–734. doi: 10.1016/0042-6822(72)90529-6. [DOI] [PubMed] [Google Scholar]
  12. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  13. McClain K., Kirsten W. H. Mouse leukemia virus growth in mouse cells contaminated with Mycoplasma. Cancer Res. 1974 Feb;34(2):281–285. [PubMed] [Google Scholar]
  14. McDugald L. V., Panem S., Kirsten W. H. Structural proteins of murine erythroblastosis virus. Int J Cancer. 1970 Jan 15;5(1):64–71. doi: 10.1002/ijc.2910050109. [DOI] [PubMed] [Google Scholar]
  15. Panem S., Kirsten W. H. Release of mouse leukemia-sarcoma virus from synchronized cells. J Natl Cancer Inst. 1973 Feb;50(2):563–565. doi: 10.1093/jnci/50.2.563. [DOI] [PubMed] [Google Scholar]
  16. Panem S. Proteins of Kirsten murine leukaemia-sarcoma virus: localization within the virus particle by iodination and fractionation techniques. J Gen Virol. 1975 Jan;26(1):1–14. doi: 10.1099/0022-1317-26-1-1. [DOI] [PubMed] [Google Scholar]
  17. Samson A. C., Fox C. F. Selective inhibition of Newcastle disease virus-induced glycoprotein synthesis by D-glucosamine hydrochloride. J Virol. 1974 Apr;13(4):775–779. doi: 10.1128/jvi.13.4.775-779.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Scholtissek C. Detection of an unstable RNA in chick fibroblasts after reduction of the UTP pool by glucosamine. Eur J Biochem. 1971 Dec;24(2):358–365. doi: 10.1111/j.1432-1033.1971.tb19694.x. [DOI] [PubMed] [Google Scholar]
  19. Scholtissek C., Rott R., Hau G., Kaluza G. Inhibition of the multiplication of vesicular stomatitis and Newcastle disease virus by 2-deoxy-d-glucose. J Virol. 1974 Jun;13(6):1186–1193. doi: 10.1128/jvi.13.6.1186-1193.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Schwarz R. T., Klenk H. D. Inhibition of glycosylation of the influenza virus hemagglutinin. J Virol. 1974 Nov;14(5):1023–1034. doi: 10.1128/jvi.14.5.1023-1034.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Somers K. D., Kirsten W. H. Long-term propagation of a murine erythroblastosis virus in vitro. J Natl Cancer Inst. 1968 May;40(5):1053–1065. [PubMed] [Google Scholar]
  22. Somers K. D., Kirsten W. H. Murine sarcoma virus: titration patterns and interference by a murine leukemia virus. Int J Cancer. 1969 Sep 15;4(5):697–704. doi: 10.1002/ijc.2910040516. [DOI] [PubMed] [Google Scholar]
  23. Somers K., Kit S. Clonal isolation of murine sarcoma virus (MSV): characterization of virus produced from transformed cells. Virology. 1971 Dec;46(3):774–785. doi: 10.1016/0042-6822(71)90079-1. [DOI] [PubMed] [Google Scholar]
  24. Temin H. M., Mizutani S. RNA-dependent DNA polymerase in virions of Rous sarcoma virus. Nature. 1970 Jun 27;226(5252):1211–1213. doi: 10.1038/2261211a0. [DOI] [PubMed] [Google Scholar]

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