Abstract
The fate of polyoma form IDNA generated during replication was investigated in resting BALB-3T3 cells. The experiments showed that there was extensive re-entry of such molecules into replication. This process took place over a period of several hours and appeared to be random. Progeny form I molecules must, therefore, spend some time in a nonreplicating pool before reinitiating replication. We propose that two factors affect the fate of progeny form IDNA. (i) The rate of reinitiation of progeny molecules is determined by the capacity of the initiation machinery. (ii) The extent of re-entry is determined by the availability of maturation proteins which divert form I from replication.
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Selected References
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- Aaronson S. A., Todaro G. J. Development of 3T3-like lines from Balb-c mouse embryo cultures: transformation susceptibility to SV40. J Cell Physiol. 1968 Oct;72(2):141–148. doi: 10.1002/jcp.1040720208. [DOI] [PubMed] [Google Scholar]
- Ben-Porat T., Coto C., Kaplan A. S. Unstable DNA synthesized by polyoma virus-infected cells. Virology. 1966 Sep;30(1):74–81. doi: 10.1016/s0042-6822(66)81011-5. [DOI] [PubMed] [Google Scholar]
- Bourgaux P., Bourgaux-Ramoisy D., Dulbecco R. The replication of the ring-shaped DNA of polyoma virus. I. Identification of the replicative intermediate. Proc Natl Acad Sci U S A. 1969 Oct;64(2):701–708. doi: 10.1073/pnas.64.2.701. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Calothy G., Hirai K., Defendi V. 5-Bromodeoxyuridine incorporation into simian virus 40 deoxyribonucleic acid. Effects on simian virus 40 replication in monkey cells. Virology. 1973 Oct;55(2):329–338. doi: 10.1016/0042-6822(73)90172-4. [DOI] [PubMed] [Google Scholar]
- Hirt B. Evidence for semiconservative replication of circular polyoma DNA. Proc Natl Acad Sci U S A. 1966 Apr;55(4):997–1004. doi: 10.1073/pnas.55.4.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Manteuil S., Pages J., Stehelin D., Girard M. Replication of simian virus 40 deoxyribonucleic acid: analysis of the one-step growth cycle. J Virol. 1973 Jan;11(1):98–106. doi: 10.1128/jvi.11.1.98-106.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REICHARD P., CANELLAKIS Z. N., CANELLAKIS E. S. Studies on a possible regulatory mechanism for the biosynthesis of deoxyribonucleic acid. J Biol Chem. 1961 Sep;236:2514–2519. [PubMed] [Google Scholar]
- Roman A., Champoux J. J., Dulbecco R. Characterization of the replicative intermediates of polyoma virus. Virology. 1974 Jan;57(1):147–160. doi: 10.1016/0042-6822(74)90116-0. [DOI] [PubMed] [Google Scholar]
- VOGT M., DULBECCO R. Studies on cells rendered neoplastic by polyoma virus: the problem of the presence of virus-related materials. Virology. 1962 Jan;16:41–51. doi: 10.1016/0042-6822(62)90200-3. [DOI] [PubMed] [Google Scholar]
- WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
- Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]