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. 1975 Jul;16(1):94–100. doi: 10.1128/jvi.16.1.94-100.1975

Role of gene 2 in bacteriophage T7 DNA synthesis.

M S Center
PMCID: PMC354636  PMID: 1133879

Abstract

Studies have been carried out to elucidate the in vivo function of gene 2 in T7 DNA synthesis. In gene 2-infected cells the rate of incorporation of (3-H)thymidine into acid-insoluble material is about 60% that of cells infected with T7 wild type. Gene 2 mutants do not however produce viable phage after infection of the nonpermissive host. In T7 wild type-infected cells, a major portion of the newly alkaline sucrose gradients. The concatemers serve as precursors for the formation of mature T7 DNA as demonstrated in pulse-chase experiments. In similar studies carried out with gene 2-infected cells, concatemers are not detected when the intracellular DNA is analyzed at several different times during the infection process. The DNA made during a gene 2 infection is present as duplex structures with a sedimentation rate close to mature T7 DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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