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. 1975 Aug;16(2):322–329. doi: 10.1128/jvi.16.2.322-329.1975

Abortive infection of a rabbit cornea cell line by vesicular stomatitis virus: conversion to productive infection by superinfection with vaccinia virus.

H R Thacore, J S Youngner
PMCID: PMC354671  PMID: 168405

Abstract

An abortive infection of a rabbit cornea cell line (RC-60) by vesicular stomatitis virus (VSV), yielding less than 1 PFU/cell, was converted to a productive infection, yielding 1,900 PFU/cell, when cells were superinfected with vaccinia. Studies on the synthesis of VSV-directed RNA in RC-60 cells suggest that the abortive infection by VSV alone may be due in part to (i) a limited production of 40S virion RNA and (ii) a markedly reduced activity of virion-bound transcriptase activity in RC-60 cells compared to the activity in mouse L cells, a permissive host for VSV. No recognizable VSV structures, except a small amount of viral core structures, were produced by the abortive infection. In contrast, double infection of RC-60 cells with VSV and vaccinia in the presence of hydroxyurea resulted in the production of infective B particles of VSV. Although the function supplied by vaccinia responsible for the productive replication of VSV in double infected RC-60 cells has not been identified, metabolic inhibitor studies indicate that continuous vaccinia-dependent RNA synthesis is required for maximal production of infective VSV. The possibility is considered that vaccinia may supply a product or function required for VSV replication which is ordinarily supplied by the host but which is lacking in RC-60 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Marcus P. I., Engelhardt D. L., Hunt J. M., Sekellick M. J. Interferon action: inhibition of vesicular stomatitis virus RNA synthesis induced by virion-bound polymerase. Science. 1971 Nov 5;174(4009):593–598. doi: 10.1126/science.174.4009.593. [DOI] [PubMed] [Google Scholar]
  2. Marcus P. I., Sekellick M. J. Cell killing by viruses. I. Comparison of cell-killing, plaque-forming, and defective-interfering particles of vesicular stomatitis virus. Virology. 1974 Feb;57(2):321–338. doi: 10.1016/0042-6822(74)90172-x. [DOI] [PubMed] [Google Scholar]
  3. Nowakowski M., Bloom B. R., Ehrenfeld E., Summers D. F. Restricted replication of vesicular stomatitis virus in human lymphoblastoid cells. J Virol. 1973 Dec;12(6):1272–1278. doi: 10.1128/jvi.12.6.1272-1278.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Schaffer F. L., Hackett A. J., Soergel M. E. Vesicular stomatitis virus RNA: complementarity between infected cell RNA and RNA's from infectious and autointerfering viral fractions. Biochem Biophys Res Commun. 1968 Jun 10;31(5):685–692. doi: 10.1016/0006-291x(68)90616-5. [DOI] [PubMed] [Google Scholar]
  5. Schincariol A. L., Howatson A. F. Replication of vesicular stomatitis virus. I. Viral specific RNA and nucleoprotein in infected L cells. Virology. 1970 Nov;42(3):732–743. doi: 10.1016/0042-6822(70)90319-3. [DOI] [PubMed] [Google Scholar]
  6. Simpson R. W., Obijeski J. F. Conditional lethal mutants of vesicular stomatitis virus. I. Phenotypic characterization of single and double mutants exhibiting host restriction and temperature sensitivity. Virology. 1974 Feb;57(2):357–368. doi: 10.1016/0042-6822(74)90175-5. [DOI] [PubMed] [Google Scholar]
  7. Soria M., Huang A. S. Association of polyadenylic acid with messenger RNA of vesicular stomatitis virus. J Mol Biol. 1973 Jul 5;77(3):449–455. doi: 10.1016/0022-2836(73)90450-6. [DOI] [PubMed] [Google Scholar]
  8. Thacore H. R., Youngner J. S. Persistence of vesicular stomatitis virus in interferon-treated cell cultures. Virology. 1975 Feb;63(2):345–351. doi: 10.1016/0042-6822(75)90308-6. [DOI] [PubMed] [Google Scholar]
  9. Thacore H. R., Youngner J. S. Rescue of vesicular stomatitis virus from interferon-induced resistance by superinfection with vaccinia virus. I. Rescue in cell cultures from different species. Virology. 1973 Dec;56(2):505–511. doi: 10.1016/0042-6822(73)90053-6. [DOI] [PubMed] [Google Scholar]
  10. Thacore H. R., Youngner J. S. Rescue of vesicular stomatitis virus from interferon-induced resistance by superinfection with vaccinia virus. II. Effect of UV-inactivated vaccinia and metabolic inhibitors. Virology. 1973 Dec;56(2):512–522. doi: 10.1016/0042-6822(73)90054-8. [DOI] [PubMed] [Google Scholar]
  11. Thacore H. R., Youngner J. S. Viral ribonuclei acid synthesis by Newcastle disease virus mutants isolated from persistently infected L cells: effect of interferon. J Virol. 1972 Mar;9(3):503–509. doi: 10.1128/jvi.9.3.503-509.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wertz G. W., Youngner J. S. Interferon production and inhibition of host synthesis in cells infected with vesicular stomatitis virus. J Virol. 1970 Oct;6(4):476–484. doi: 10.1128/jvi.6.4.476-484.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wild T. F. Replication of vesicular stomatitis virus: characterization of the virus-induced RNA. J Gen Virol. 1971 Nov;13(2):295–310. doi: 10.1099/0022-1317-13-2-295. [DOI] [PubMed] [Google Scholar]
  14. Youngner J. S., Scott A. W., Hallum J. V., Stinebring W. R. Interferon production by inactivated Newcastle disease virus in cell cultures and in mice. J Bacteriol. 1966 Oct;92(4):862–868. doi: 10.1128/jb.92.4.862-868.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Youngner J. S., Thacore H. R., Kelly M. E. Sensitivity of ribonucleic acid and deoxyribonucleic acid viruses to different species of interferon in cell cultures. J Virol. 1972 Aug;10(2):171–178. doi: 10.1128/jvi.10.2.171-178.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]

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