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. 1975 Sep;16(3):621–633. doi: 10.1128/jvi.16.3.621-633.1975

Biochemical studies on bovine adenovirus type 3. I. Purification and properties.

Y Niiyama, K Igarashi, K Tsukamoto, T Kurokawa, Y Sugino
PMCID: PMC354710  PMID: 1171993

Abstract

Bovine adenovirus type 3 (BAV3) was purified and its properties were studied. On productive infection of CKT1 cells (a cell line derived from calf kidney) with BAV3, it was observed that viral DNA synthesis was initiated after about 24 h and its rate was maximal after about 40 h. Maturation of the virus occurred several hours after this. Purified BAV3 was separated into four discrete bands by CsCl density gradient centrifugation (complete, incomplete, empty, and degraded viruses). The complete BAV3 was similar in size and structure to human and avian adenoviruses. Polyacrylamide gel electrophoresis showed that the complete BAV3 virion contained at least 10 polypeptides. The total structural proteins of the virion had a similar amino acid composition to those of human adenoviruses. DNA of the complete virus was a linear duplex and its contour length was 12.3 +/- 0.9 mum. The So20,w value of the DNA was 32.9S and its buoyant density in CsCl was 1.717 g/ml. There was about 25% homology between the DNAs of BAV3 and human adenovirus type 5 by filter hybridization. It was also noted that BAV3 produced incomplete virus. The incomplete virus was similar in morphology to the complete virus and contained almost all the structural polypeptides of the latter, but lacked infectivity. However, its DNA had a deletion(s) (13%) which seemed to locate near a terminal.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  2. Burlingham B. T., Brown D. T., Doerfler W. Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirions of types 2 and 12. Virology. 1974 Aug;60(2):419–430. doi: 10.1016/0042-6822(74)90336-5. [DOI] [PubMed] [Google Scholar]
  3. Darbyshire J. H., Berman L. D., Chesterman F. C., Pereira H. G. Studies on the oncogenicity of bovine adenovirus type 3. Int J Cancer. 1968 Sep 15;3(5):546–557. doi: 10.1002/ijc.2910030503. [DOI] [PubMed] [Google Scholar]
  4. Darbyshire J. H., Dawson P. S., Lamont P. H., Ostler D. C., Pereira H. G. A new adenovirus serotype of bovine origin. J Comp Pathol. 1965 Jul;75(3):327–330. doi: 10.1016/0021-9975(65)90038-1. [DOI] [PubMed] [Google Scholar]
  5. Darbyshire J. H. Oncogenicity of bovine adenovirus type 3 in hamsters. Nature. 1966 Jul 2;211(5044):102–102. doi: 10.1038/211102a0. [DOI] [PubMed] [Google Scholar]
  6. Doerfler W. Nonproductive infection of baby hamster kidney cells (BHK21) with adenovirus type 12. Virology. 1969 Aug;38(4):587–606. doi: 10.1016/0042-6822(69)90179-2. [DOI] [PubMed] [Google Scholar]
  7. Everitt E., Sundquist B., Pettersson U., Philipson L. Structural proteins of adenoviruses. X. Isolation and topography of low molecular weight antigens from the virion of adenovirus type 2. Virology. 1973 Mar;52(1):130–147. doi: 10.1016/0042-6822(73)90404-2. [DOI] [PubMed] [Google Scholar]
  8. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  10. Garon C. F., Berry K. W., Rose J. A. A unique form of terminal redundancy in adenovirus DNA molecules. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2391–2395. doi: 10.1073/pnas.69.9.2391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Green M. Effect of oncogenic DNA viruses on regulatory mechanisms of cells. Fed Proc. 1970 May-Jun;29(3):1265–1275. [PubMed] [Google Scholar]
  12. Green M., Piña M., Kimes R., Wensink P. C., MacHattie L. A., Thomas C. A., Jr Adenovirus DNA. I. Molecular weight and conformation. Proc Natl Acad Sci U S A. 1967 May;57(5):1302–1309. doi: 10.1073/pnas.57.5.1302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Ishibashi M., Maizel J. V., Jr The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology. 1974 Feb;57(2):409–424. doi: 10.1016/0042-6822(74)90181-0. [DOI] [PubMed] [Google Scholar]
  14. KLEIN M., EARLEY E., ZELLAT J. Isolation from cattle of a virus related to human adenovirus. Proc Soc Exp Biol Med. 1959 Oct;102:1–4. doi: 10.3181/00379727-102-25123. [DOI] [PubMed] [Google Scholar]
  15. LACY S., GREEN M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION. VII. HOMOLOGY BETWEEN DNA'S OF TUMORIGENIC AND NONTUMORIGENIC HUMAN ADENOVIRUSES. Proc Natl Acad Sci U S A. 1964 Oct;52:1053–1059. doi: 10.1073/pnas.52.4.1053. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ledinko N., Fong C. K. Kinetics of nucleic acid synthesis in human embryonic kidney cultures infected with adenovirus 2 or 12: inhibition of cellular deoxyribonucleic acid synthesis. J Virol. 1969 Aug;4(2):123–132. doi: 10.1128/jvi.4.2.123-132.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology. 1968 Sep;36(1):115–125. doi: 10.1016/0042-6822(68)90121-9. [DOI] [PubMed] [Google Scholar]
  18. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  19. Mak S. Defective virions in human adenovirus type 12. J Virol. 1971 Apr;7(4):426–433. doi: 10.1128/jvi.7.4.426-433.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Motoi M., Fukui H., Ogawa K. Neoplastic transformation of hamster cells in vitro by bovine adenovirus type-3. Gan. 1972 Aug;63(4):415–418. [PubMed] [Google Scholar]
  21. Nishibe Y., Kimura T., Inoue Y. K. Clonal analysis of tumorigenesis of bovine adenovirus type 3 and isolation of a nontumorigenic variant. Arch Gesamte Virusforsch. 1970;29(2):195–204. doi: 10.1007/BF01249305. [DOI] [PubMed] [Google Scholar]
  22. Norrby E. The structural and functional diversity of Adenovirus capsid components. J Gen Virol. 1969 Sep;5(2):221–236. doi: 10.1099/0022-1317-5-2-221. [DOI] [PubMed] [Google Scholar]
  23. OUCHTERLONY O. Diffusion-in-gel methods for immunological analysis. II. Prog Allergy. 1962;6:30–154. doi: 10.1159/000313795. [DOI] [PubMed] [Google Scholar]
  24. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Pettersson U., Philipson L., Höglund S. Structural proteins of adenoviruses. I. Purification and characterization of the adenovirus type 2 hexon antigen. Virology. 1967 Dec;33(4):575–590. doi: 10.1016/0042-6822(67)90057-8. [DOI] [PubMed] [Google Scholar]
  26. Piña M., Green M. Base composition of the DNA of oncogenic simian adenovirus SA7 and homology with human adenovirus DNA's. Virology. 1968 Oct;36(2):321–323. doi: 10.1016/0042-6822(68)90154-2. [DOI] [PubMed] [Google Scholar]
  27. Piña M., Green M. Biochemical studies on adenovirus multiplication. IX. Chemical and base composition analysis of 28 human adenoviruses. Proc Natl Acad Sci U S A. 1965 Aug;54(2):547–551. doi: 10.1073/pnas.54.2.547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Polasa H., Green M. Adenovirus proteins. I. Amino acid composition of oncogenic and nononcogenic human adenoviruses. Virology. 1967 Mar;31(3):565–567. doi: 10.1016/0042-6822(67)90242-5. [DOI] [PubMed] [Google Scholar]
  29. Prage L., Höglund S., Philipson L. Structural proteins of adenoviruses. 8. Characterization of incomplete particles of adenovirus type 3. Virology. 1972 Sep;49(3):745–757. doi: 10.1016/0042-6822(72)90531-4. [DOI] [PubMed] [Google Scholar]
  30. Rosenwirth B., Tjia S., Westphal M., Doerfler W. Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology. 1974 Aug;60(2):431–437. doi: 10.1016/0042-6822(74)90337-7. [DOI] [PubMed] [Google Scholar]
  31. Russell W. C., Skehel J. J. The polypeptides of adenovirus type 12. J Gen Virol. 1973 Aug;20(2):195–209. doi: 10.1099/0022-1317-20-2-195. [DOI] [PubMed] [Google Scholar]
  32. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  33. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  34. Schlesinger R. W. Adenoviruses: the nature of the virion and of controlling factors in productive or abortive infection and tumorigenesis. Adv Virus Res. 1969;14:1–61. doi: 10.1016/s0065-3527(08)60556-4. [DOI] [PubMed] [Google Scholar]
  35. Sundquist B., Everitt E., Philipson L., Hoglund S. Assembly of adenoviruses. J Virol. 1973 Mar;11(3):449–459. doi: 10.1128/jvi.11.3.449-459.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. THOMAS C. A., Jr, BERNS K. I. The physical characterization of DNA molecules released from T2 and T4 bacteriophage. J Mol Biol. 1961 Jun;3:277–288. doi: 10.1016/s0022-2836(61)80069-7. [DOI] [PubMed] [Google Scholar]
  37. Thomas C. A., Jr The arrangement of information in DNA molecules. J Gen Physiol. 1966 Jul;49(6):143–169. doi: 10.1085/jgp.49.6.143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Tsukamoto K., Sugino Y. Nonproductive infection and induction of cellular deoxyribonucleic acid synthesis by bovine adenovirus type 3 in a contact-inhibited mouse cell line. J Virol. 1972 Mar;9(3):465–473. doi: 10.1128/jvi.9.3.465-473.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. VINOGRAD J., BRUNER R., KENT R., WEIGLE J. Band-centrifugation of macromolecules and viruses in self-generating density gradients. Proc Natl Acad Sci U S A. 1963 Jun;49:902–910. doi: 10.1073/pnas.49.6.902. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Wadell G., Hammarskjöld M. L., Varsanyi T. Incomplete virus particles of adenovirus type 16. J Gen Virol. 1973 Sep;20(3):287–302. doi: 10.1099/0022-1317-20-3-287. [DOI] [PubMed] [Google Scholar]
  41. Warmaar S. O., Cohen J. A. A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem Biophys Res Commun. 1966 Aug 23;24(4):554–558. doi: 10.1016/0006-291x(66)90356-1. [DOI] [PubMed] [Google Scholar]
  42. Williams J. F. Enhancement of adenovirus plaque formation on HeLa cells by magnesium chloride. J Gen Virol. 1970 Dec;9(3):251–255. doi: 10.1099/0022-1317-9-3-251. [DOI] [PubMed] [Google Scholar]
  43. Yoshikura H., Hirokawa Y., Yamada M. Synchronized cell division induced by medium change. Exp Cell Res. 1967 Oct;48(1):226–228. doi: 10.1016/0014-4827(67)90309-6. [DOI] [PubMed] [Google Scholar]

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