Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1975 Sep;16(3):634–641. doi: 10.1128/jvi.16.3.634-641.1975

Biochemical studies on bovine adenovirus type 3. II. Incomplete virus.

K Igarashi, Y Niiyama, K Tsukamoto, T Kurokawa, Y Sugino
PMCID: PMC354711  PMID: 1171994

Abstract

Incomplete virus of oncogenic bovine adenovirus type 3 (BAV3) was highly purified and its biological activity was studied. The production of incomplete virus was found to increase with a high multiplicity of infection and with a large amount of arginine in the growth medium. On infection of contact-inhibited mouse cells, incomplete virus induced cellular DNA synthesis and focus formation. Moreover, this virus was oncogenic to newborn hamsters. On infection of calf kidney cells, a permissive cell line, viral early and late RNA, viral DNA, and almost all the viral late proteins were produced, but no mature progeny virus was detected. It is, therefore, suggested that incomplete virus of BAV3 may be unable to synthesize a protein(s) (perhaps a kind of maturation protein[s]) essential for assembly of viral macromolecules for maturation.

Full text

PDF
634

Images in this article

639Image
on p.639

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blackstein M. E., Stanners C. P., Farmilo A. J. Heterogeneity of polyoma virus DNA: isolation and characterization of non-infectious small supercoiled molecules. J Mol Biol. 1969 Jun 14;42(2):301–313. doi: 10.1016/0022-2836(69)90045-x. [DOI] [PubMed] [Google Scholar]
  2. Brockman W. W., Lee T. N., Nathans D. The evolution of new species of viral DNA during serial passage of simian virus 40 at high multiplicity. Virology. 1973 Aug;54(2):384–397. doi: 10.1016/0042-6822(73)90151-7. [DOI] [PubMed] [Google Scholar]
  3. Brockman W. W., Nathans D. The isolation of simian virus 40 variants with specifically altered genomes. Proc Natl Acad Sci U S A. 1974 Mar;71(3):942–946. doi: 10.1073/pnas.71.3.942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burlingham B. T., Brown D. T., Doerfler W. Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirions of types 2 and 12. Virology. 1974 Aug;60(2):419–430. doi: 10.1016/0042-6822(74)90336-5. [DOI] [PubMed] [Google Scholar]
  5. Burlingham B. T., Doerfler W., Pettersson U., Philipson L. Adenovirus endonuclease: association with the penton of adenovirus type 2. J Mol Biol. 1971 Aug 28;60(1):45–64. doi: 10.1016/0022-2836(71)90446-3. [DOI] [PubMed] [Google Scholar]
  6. Butel J. S., Melnick J. L., Rapp F. Detection of biologically active adenovirions unable to plaque in human cells. J Bacteriol. 1966 Aug;92(2):433–438. doi: 10.1128/jb.92.2.433-438.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CRAWFORD L. V., CRAWFORD E. M., WATSONDH The physical characteristics of polyoma virus. I. Two types of particle. Virology. 1962 Oct;18:170–176. doi: 10.1016/0042-6822(62)90002-8. [DOI] [PubMed] [Google Scholar]
  8. Darbyshire J. H. Oncogenicity of bovine adenovirus type 3 in hamsters. Nature. 1966 Jul 2;211(5044):102–102. doi: 10.1038/211102a0. [DOI] [PubMed] [Google Scholar]
  9. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  10. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  11. Hedgpeth J., Goodman H. M., Boyer H. W. DNA nucleotide sequence restricted by the RI endonuclease. Proc Natl Acad Sci U S A. 1972 Nov;69(11):3448–3452. doi: 10.1073/pnas.69.11.3448. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hewish D. R., Burgoyne L. A. Chromatin sub-structure. The digestion of chromatin DNA at regularly spaced sites by a nuclear deoxyribonuclease. Biochem Biophys Res Commun. 1973 May 15;52(2):504–510. doi: 10.1016/0006-291x(73)90740-7. [DOI] [PubMed] [Google Scholar]
  13. Huang A. S., Baltimore D. Defective viral particles and viral disease processes. Nature. 1970 Apr 25;226(5243):325–327. doi: 10.1038/226325a0. [DOI] [PubMed] [Google Scholar]
  14. Huang A. S., Greenawalt J. W., Wagner R. R. Defective T particles of vesicular stomatitis virus. I. Preparation, morphology, and some biologic properties. Virology. 1966 Oct;30(2):161–172. doi: 10.1016/0042-6822(66)90092-4. [DOI] [PubMed] [Google Scholar]
  15. Ishibashi M., Maizel J. V., Jr The polypeptides of adenovirus. V. Young virions, structural intermediate between top components and aged virions. Virology. 1974 Feb;57(2):409–424. doi: 10.1016/0042-6822(74)90181-0. [DOI] [PubMed] [Google Scholar]
  16. Kimura S., Kamogashira T., Ota F., Fukui K., Yoshida N. Growth of bovine adenovirus type 3 in cells cloned from a cell line of calf kidney. Tokushima J Exp Med. 1971 Oct;18:33–37. [PubMed] [Google Scholar]
  17. Kingsbury D. W., Portner A., Darlington R. W. Properties of incomplete Sendai virions and subgenomic viral RNAs. Virology. 1970 Dec;42(4):857–871. doi: 10.1016/0042-6822(70)90335-1. [DOI] [PubMed] [Google Scholar]
  18. Lavi S., Rozenblatt S., Singer M. F., Winocour E. Acquisition of sequences homologous to host DNA by closed circular simian virus 40 DNA. II. Further studies on the serial passage of virus clones. J Virol. 1973 Sep;12(3):492–500. doi: 10.1128/jvi.12.3.492-500.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Maizel J. V., Jr, White D. O., Scharff M. D. The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology. 1968 Sep;36(1):126–136. doi: 10.1016/0042-6822(68)90122-0. [DOI] [PubMed] [Google Scholar]
  20. Mak S. Defective virions in human adenovirus type 12. J Virol. 1971 Apr;7(4):426–433. doi: 10.1128/jvi.7.4.426-433.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Neville D. M., Jr Molecular weight determination of protein-dodecyl sulfate complexes by gel electrophoresis in a discontinuous buffer system. J Biol Chem. 1971 Oct 25;246(20):6328–6334. [PubMed] [Google Scholar]
  22. Nonoyama M., Watanabe Y., Graham A. F. Defective virions of reovirus. J Virol. 1970 Aug;6(2):226–236. doi: 10.1128/jvi.6.2.226-236.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Prage L., Höglund S., Philipson L. Structural proteins of adenoviruses. 8. Characterization of incomplete particles of adenovirus type 3. Virology. 1972 Sep;49(3):745–757. doi: 10.1016/0042-6822(72)90531-4. [DOI] [PubMed] [Google Scholar]
  24. Rainbow A. J., Mak S. Functional heterogeneity of virions in human adenovirus types 2 and 12. J Virol. 1970 Feb;5(2):188–193. doi: 10.1128/jvi.5.2.188-193.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Rosenwirth B., Tjia S., Westphal M., Doerfler W. Incomplete particles of adenovirus. II. Kinetics of formation and polypeptide composition of adenovirus type 2. Virology. 1974 Aug;60(2):431–437. doi: 10.1016/0042-6822(74)90337-7. [DOI] [PubMed] [Google Scholar]
  26. Rouse H. C., Schlesinger R. W. An arginine-dependent step in the maturation of type 2 adenovirus. Virology. 1967 Nov;33(3):513–522. doi: 10.1016/0042-6822(67)90128-6. [DOI] [PubMed] [Google Scholar]
  27. SCHERRER K., DARNELL J. E. Sedimentation characteristics of rapidly labelled RNA from HeLa cells. Biochem Biophys Res Commun. 1962 Jun 4;7:486–490. doi: 10.1016/0006-291x(62)90341-8. [DOI] [PubMed] [Google Scholar]
  28. SMITH K. O. STUDIES ON ADENOVIRUS-12. I. QUANTITATIVE CORRELATIONS BETWEEN SOME PHYSICAL, ANTIGENIC AND INFECTIOUS PROPERTIES. J Immunol. 1965 Jun;94:976–989. [PubMed] [Google Scholar]
  29. Shimojo H., Yamashita T., Yamamoto H., Suzuki E., Abe C. Empty particles of Adenovirus 12. Jpn J Med Sci Biol. 1967 Apr;20(2):199–203. doi: 10.7883/yoken1952.20.199. [DOI] [PubMed] [Google Scholar]
  30. Tai H. T., Smith C. A., Sharp P. A., Vinograd J. Sequence heterogeneity in closed simian virus 40 deoxyribonucleic acid. J Virol. 1972 Feb;9(2):317–325. doi: 10.1128/jvi.9.2.317-325.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tsukamoto K., Sugino Y. Nonproductive infection and induction of cellular deoxyribonucleic acid synthesis by bovine adenovirus type 3 in a contact-inhibited mouse cell line. J Virol. 1972 Mar;9(3):465–473. doi: 10.1128/jvi.9.3.465-473.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Uchida S., Watanabe S., Kato M. Incomplete growth of simian virus 40 in African green monkey kidney culture induced by serial undiluted passages. Virology. 1966 Jan;28(1):135–141. doi: 10.1016/0042-6822(66)90314-x. [DOI] [PubMed] [Google Scholar]
  33. Wadell G., Hammarskjöld M. L., Varsanyi T. Incomplete virus particles of adenovirus type 16. J Gen Virol. 1973 Sep;20(3):287–302. doi: 10.1099/0022-1317-20-3-287. [DOI] [PubMed] [Google Scholar]
  34. Warmaar S. O., Cohen J. A. A quantitative assay for DNA-DNA hybrids using membrane filters. Biochem Biophys Res Commun. 1966 Aug 23;24(4):554–558. doi: 10.1016/0006-291x(66)90356-1. [DOI] [PubMed] [Google Scholar]
  35. Yoshiike K. Studies on DNA from low-density particles of SV40. I. Heterogeneous defective virions produced by successive undiluted passages. Virology. 1968 Mar;34(3):391–401. doi: 10.1016/0042-6822(68)90059-7. [DOI] [PubMed] [Google Scholar]
  36. Yoshikura H., Hirokawa Y., Yamada M. Synchronized cell division induced by medium change. Exp Cell Res. 1967 Oct;48(1):226–228. doi: 10.1016/0014-4827(67)90309-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES